The Impact of Laparoscopy with Carbon Dioxide versus Helium on Immunologic Function and Tumor Growth in a Rat Model

Jacobi, C. A.; Wenger, F. A.; Sabat, Robert; Volk, Thomas; Ordemann, J.; Müller, J. M.

doi:10.1159/000018603

Cited by 72 publications

(55 citation statements)

References 8 publications

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“…The bio-Schulz/Rodriguez/Mutters/Menendez/Bette Lymph node mesenterical TNF-mRNA = logical mechanism for this is still unknown, but it might be due to an altered immune status generated by ozonemediated priming effects. It is well known that different gases insufflated into the peritoneum can influence the intraperitoneal immunity [26,27]. Chekan et al [28] showed that a CO 2 pneumoperitoneum has been implicated as a possible factor in depressed intraperitoneal immunity with detrimental effects on impaired peritoneal clearance of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Repetitive Pneumoperitoneum with Ozonized Oxygen as a Preventive in Lethal Polymicrobial Sepsis in Rats

Schulz

Rodriguez²,

Mutters³

et al. 2003

Eur Surg Res

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The aim of this study was to test whether repetitive pretreatments of rats with ozonized oxygen at relatively low gas volumes into the abdomen (20 ml per rat per day) have any beneficial or detrimental effects on the course of a polymicrobial-induced lethal peritonitis. Peritonitis was induced in a surgical or a nonsurgical model by usage of fecal material from the cecum. As the biological read out we used the mortality analysis. To include possible mechanisms by which ozone might influence the septic outcome, we characterized the gene expression of the pro-inflammatory cytokines IL-1β, IL-2, and TNF-α mRNA in lymphoid organs. In both models, we found a significant beneficial influence of a dose-dependent O₂/O₃pneumoperitoneum on the survival rate when compared to control animals or to room air. The ozone-enhanced survival seems to be independent from altered cytokine expression because there were no differences noticed in the levels of bacterial-induced gene expression of IL-1β and TNF-α in septic animals pretreated with ozonized oxygen when compared to control animals.

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Section: Discussionmentioning

confidence: 99%

Repetitive Pneumoperitoneum with Ozonized Oxygen as a Preventive in Lethal Polymicrobial Sepsis in Rats

Schulz

Rodriguez²,

Mutters³

et al. 2003

Eur Surg Res

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“…Nevertheless, intraoperative release of growth-stimulating cytokines, like IL-1 [16, 17, 33], by peritoneal macrophages seems to be important for intra- and extraperitoneal tumor cell growth. Thus, it has been reported that preoperative treatment with anti-IL-1 caused a significant decrease in peritoneal adhesion formation in a rat model [34].…”

Section: Discussionmentioning

confidence: 99%

New Therapeutic Strategies to Avoid Intra- and Extraperitoneal Metastases during Laparoscopy: Results of a Tumor Model in the Rat

et al. 1999

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Background: Therapeutic strategies to prevent port site recurrences in laparoscopy surgery of malignancies have not been investigated until now. Methods: The effects of taurolidine, heparin, and povidone iodine on the growth of rat and human colon adenocarcinoma as well as gallbladder carcinoma were investigated in vitro. Furthermore, cytokine release of growth-stimulating IL-1β by peritoneal macrophages was measured after incubation with carbon dioxide and additional incubation with the different agents. In the third experiment, prevention of intra- and extraperitoneal metastases by intraperitoneal instillation of the different agents during laparoscopy was investigated in a colon carcinoma model in the rat. Tumor cells were administered intraperitoneally in 100 rats, and pneumoperitoneum (8 mm Hg) was established over 30 min with carbon dioxide. Rats received either tumor cells, cells + heparin, cells + povidone iodine, cells + taurolidine, or cells + taurolidine + heparin. Results: In vitro, tumor cell growth decreased after incubation with taurolidine, taurolidine/heparin, and povidone iodine. Cytokine release was stimulated by incubation with carbon dioxide and could only be suppressed by incubation with taurolidine in vitro. In vivo, intraperitoneal tumor weight was lower in rats receiving heparin (251 ± 153 mg) and povidone iodine (134 ± 117 mg) compared to the control group (541 ± 291 mg), but even less when taurolidine (79 ± 82 mg) or taurolidine/heparin (18.3 ± 30 mg) were instilled. Conclusion: Heparin slightly inhibits intraperitoneal tumor growth in vivo, while povidone iodine and taurolidine cause a significant decrease in tumor cell growth in vitro as well as intraperitoneal tumor growth in vivo. Cytokine release of peritoneal macrophages is only suppressed by taurolidine. Total tumor take and trocar metastases are only suppressed by taurolidine and taurolidine/heparin.

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“…Currently, an European multicentre study is ongoing for the investigation of adjuvant intraperitoneal irrigation with taurolidine after resection of colon cancer. Its effect is related to induction of apoptosis [20] and inhibition of growth-promoting cytokines as IL-1 or other anti-inflammatory cytokines as TNF-a [21] which have a stimulating effect on tumour growth [22]. Both of them are also described as key cytokines known to play a role in mesothelioma [23].…”

Section: Discussionmentioning

confidence: 99%

Local recurrence model of malignant pleural mesothelioma for investigation of intrapleural treatment☆

Opitz

Lardinois

Arni

et al. 2007

European Journal of Cardio-Thoracic Surgery

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Objective: Local recurrence remains a major problem in the treatment of malignant pleural mesothelioma. The aim of the underlying study was to establish a standardised local recurrence model in rats which enables to study different intrapleural therapies. Materials and methods: Fifty microlitre containing 1 Â 10 6 cells of a syngeneic rat malignant mesothelioma cell line (II-45), established from mesothelioma in Fischer 344 rats exposed to asbestos, were inoculated subpleurally via a left-sided thoracotomy. Tumour size was assessed 6 days later and the tumour nodule completely resected. Evaluation of recurrence at the resection site was performed after 10 days (n = 6) and 6 days (n = 6). The recurrent nodule was histopathologically confirmed. In a second experiment, this new recurrence model was evaluated for the effect of intrapleural therapy with different agents: 4 ml of cisplatin-solution (100 mg 2 /kg BW), cisplatin combined with the fibrin-based sealant Vivostat W , 4 ml taurolidine 2%, repeated injection of 1 mg of the chemokine CCL-19 at the tumour site and 4 ml povidone-iodine in a dilution 1:10. In a control group, the chest cavity was filled with 4 ml 0.9% NaCl. The primary endpoint was the extent of tumour recurrence. Results: Six days after inoculation, all animals presented a standardised tumour nodule at the injection site of a mean diameter of 5.1 (AE0.8) mm. Evaluation of the recurrence after 10 days showed a relapse directly at the resection site, but additional tumour nodules on the ipsi-and contralateral chest wall were found and histologically confirmed. The animals that were sacrificed 6 days after resection of the tumour nodule showed a recurrence only at the resection site with no macroscopic or microscopic evidence of other tumour. Resection of the tumour nodule combined with intrapleural application of the different agents lead to clear reduction of recurrence. The strongest effect was observed after intrapleural application of cisplatin-Vivostat W with significant decrease of the longest, widest and thickest diameter of the recurrence. Conclusions: With this new recurrence model for investigation of malignant pleural mesothelioma in rats, we were able to investigate new intrapleural therapies after pneumonectomy. The intrapleural application of cisplatin-Vivostat W significantly reduced the extent of local recurrence. #

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The Impact of Laparoscopy with Carbon Dioxide versus Helium on Immunologic Function and Tumor Growth in a Rat Model

Cited by 72 publications

References 8 publications

Repetitive Pneumoperitoneum with Ozonized Oxygen as a Preventive in Lethal Polymicrobial Sepsis in Rats

Repetitive Pneumoperitoneum with Ozonized Oxygen as a Preventive in Lethal Polymicrobial Sepsis in Rats

New Therapeutic Strategies to Avoid Intra- and Extraperitoneal Metastases during Laparoscopy: Results of a Tumor Model in the Rat

Local recurrence model of malignant pleural mesothelioma for investigation of intrapleural treatment☆

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