The Impact of lncRNAs and miRNAs on Apoptosis in Lung Cancer

Ghafouri‐Fard, Soudeh; Aghabalazade, Amin; Shoorei, Hamed; Majidpoor, Jamal; Taheri, Mohammad; Mokhtari, Majid

doi:10.3389/fonc.2021.714795

Cited by 16 publications

(12 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the cytotoxic effects of most oncological chemotherapeutic agents are mediated through activation of apoptotic pathways, and apoptosis targeting holds promise as a key strategy for cancer treatment ( Johnstone et al, 2002 ; Singh and Lim, 2022 ). Increasing evidence showed that lncRNAs can regulate apoptosis through different mechanisms, and their regulatory effects on apoptosis in lung cancer cells have been investigated ( Wang et al, 2020 ; Xiang et al, 2020 ; Ghafouri-Fard et al, 2021 ; Ouyang et al, 2021 ). Whereas, the role of ApoRLs in the prognosis, chemotherapy and immunotherapy of LUAD is not well understood.…”

Section: Discussionmentioning

confidence: 99%

“…Long non-coding RNAs (lncRNAs), which typically exceed 200 nucleotides in size and are transcribed by RNA polymerase II, have an important regulatory role in the induction of apoptosis ( Ghafouri-Fard et al, 2021 ). Many studies have demonstrated that lncRNAs are key regulators involved in the progression of human cancer including lung cancer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of a apoptosis-related LncRNA signature to improve prognosis prediction and immunotherapy response in lung adenocarcinoma patients

et al. 2022

View full text Add to dashboard Cite

Apoptosis is closely associated with the development of various cancers, including lung adenocarcinoma (LUAD). However, the prognostic value of apoptosis-related lncRNAs (ApoRLs) in LUAD has not been fully elucidated. In the present study, we screened 2, 960 ApoRLs by constructing a co-expression network of mRNAs-lncRNAs associated with apoptosis, and identified 421 ApoRLs that were differentially expressed between LUAD samples and normal lung samples. Sixteen differentially expressed apoptosis-related lncRNAs (DE-ApoRLs) with prognostic relevance to LUAD patients were screened using univariate Cox regression analysis. An apoptosis-related lncRNA signature (ApoRLSig ) containing 10 ApoRLs was constructed by applying the Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression method, and all LUAD patients in the TCGA cohort were divided into high or low risk groups. Moreover, patients in the high-risk group had a worse prognosis (p < 0.05). When analyzed in conjunction with clinical features, we found ApoRLSig to be an independent predictor of LUAD patients and established a prognostic nomogram combining ApoRLSig and clinical features. Gene set enrichment analysis (GSEA) revealed that ApoRLSig is involved in many malignancy-associated immunomodulatory pathways. In addition, there were significant differences in the immune microenvironment and immune cells between the high-risk and low-risk groups. Further analysis revealed that the expression levels of most immune checkpoint genes (ICGs) were higher in the high-risk group, which suggested that the immunotherapy effect was better in the high-risk group than in the low-risk group. And we found that the high-risk group was also better than the low-risk group in terms of chemotherapy effect. In conclusion, we successfully constructed an ApoRLSig which could predict the prognosis of LUAD patients and provide a novel strategy for the antitumor treatment of LUAD patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of a apoptosis-related LncRNA signature to improve prognosis prediction and immunotherapy response in lung adenocarcinoma patients

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Moreover, miRNAs can participate in the regulation of multiple signalling pathways, including the PI3K/AKT pathway [34–37]. The PI3K/AKT pathway, as a key regulatory pathway, is involved in regulating cell growth, metabolism, apoptosis, metastasis and chemotherapy resistance [38,39].…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics identification of miR‐514b‐5p promotes NSCLC progression and induces PI3K/AKT and p38 pathways by targeting small glutamine‐rich tetratricopeptide repeat‐containing protein beta

et al. 2022

View full text Add to dashboard Cite

Lung cancer is the most aggressive cancer with the highest mortality and incidence rates worldwide. MicroRNAs have been identified as potential targets for non‐small cell lung cancer (NSCLC) treatment. However, the modulatory role of miR‐514b‐5p in NSCLC progression is little known. In the present study, miRNA expression datasets for NSCLC were downloaded from the Cancer Genome Atlas and Gene Ontology Omnibus databases. Gene expression was assessed using a quantitative real‐time PCR, and western blot analysis and immunohistochemical staining was used to determine protein expression. Gain and loss of function experiments were performed to investigate the impact of miR‐514b‐5p and small glutamine‐rich tetratricopeptide repeat‐containing protein beta (SGTB) on cell proliferation and apoptosis. RNA immunoprecipitation and dual‐luciferase assays were performed to analyse the target gene of miR‐514b‐5p. The biological roles of miR‐514b‐5p were lastly evaluated using nude mouse tumorigenicity assays in vivo. We found that miR‐514b‐5p was dramatically increased in NSCLC tissues and higher miR‐514b‐5p expression was associated with poorer overall survival in NSCLC patients. Furthermore, overexpression of miR‐514b‐5p promoted NSCLC cell growth and suppressed apoptosis by inducing the activation of the phosphatidylinositol‐3‐kinase (PI3K)/AKT and p38 signalling pathways. Mechanistically, dual‐luciferase and the RNA immunoprecipitation results highlighted that SGTB was a target gene of miR‐514b‐5p. Moreover, overexpression of SGTB reduced cell division and promoted apoptosis in vitro through blocking the PI3K/AKT and p38 signalling pathways. Our findings indicated that miR‐514b‐5p contributes to carcinoma progression in NSCLC via the PI3K/AKT and p38 signalling pathways by targeting SGTB and this could be a promising diagnostic and therapeutic target for the treatment of NSCLC.

show abstract

“…For example, some miRNAs are critical in lung cancer treatment by targeting TP53-dependent cell apoptosis [ 33 ]. Previous studies also suggested that miRNAs regulating lung cancer cell apoptosis interact with many important cancer pathways, including PI3K/Akt, PTEN, GSK-3b, NF-kB, BCL2, BAX, P53, and mTOR [ 34 ]. However, the biological mechanisms underlying lung cancer-associated miRNAs are unknown.…”

Section: Roles Of Moap1 In Cancersmentioning

confidence: 99%

Revealing the Roles of MOAP1 in Diseases: A Review

Su¹,

Wang²,

Meng³

2022

Cells

View full text Add to dashboard Cite

Modulator of apoptosis protein1 (MOAP1), also known as MAP1 and PNMA4, belongs to the PNMA gene family consisting of at least 15 genes located on different chromosomes. MOAP1 interacts with the BAX protein, one of the most important apoptosis regulators. Due to its critical role in a few of disease-associated pathways, MOAP1 is associated with many diseases such as cancers and neurological diseases. In this study, we introduced MOAP1 and its biological functions and reviewed the associations between MOAP1 and a few diseases including cancers, neurological diseases, and other diseases such as inflammation and heart diseases. We also explained possible biological mechanisms underlying the associations between MOAP1 and these diseases, and discussed a few future directions regarding MOAP1, especially its potential roles in neurodegenerative disorders. In summary, MOAP1 plays a critical role in the development and progression of cancers and neurological diseases by regulating a few genes related to cellular apoptosis such as BAX and RASSF1A and interacting with disease-associated miRNAs, including miR-25 and miR1228.

show abstract

The Impact of lncRNAs and miRNAs on Apoptosis in Lung Cancer

Cited by 16 publications

References 102 publications

Identification of a apoptosis-related LncRNA signature to improve prognosis prediction and immunotherapy response in lung adenocarcinoma patients

Identification of a apoptosis-related LncRNA signature to improve prognosis prediction and immunotherapy response in lung adenocarcinoma patients

Bioinformatics identification of miR‐514b‐5p promotes NSCLC progression and induces PI3K/AKT and p38 pathways by targeting small glutamine‐rich tetratricopeptide repeat‐containing protein beta

Revealing the Roles of MOAP1 in Diseases: A Review

Contact Info

Product

Resources

About