The impact of lncRNAs MIR503HG, TTN-AS1, and FEZF1-AS1 and their association with miR-107 expression in type 2 diabetes mellitus

Abstract

Type 2 diabetes mellitus (T2DM) is a complex disease characterized by insulin resistance and insulin secretion defects. Its causes are unknown, but genetic and environmental factors play a role. Obesity and insulin resistance are associated with hsa-miR-107 dysregulation. Evidence suggests that several long non-coding RNAs (lncRNAs) are involved in gene regulation of β-cells and T2DM pathogenesis. This study evaluated the association between lncRNAs and hsa-miR-107 and then identified the proteins involved in T2DM using in silico analyses. Three lncRNAs including MIR503HG, TTN-AS1, and FEZF1-AS1 were selected based on the research literature. In this study, 80 T2DM patients and 110 healthy individuals were recruited, and their peripheral blood was collected for biochemical measurements and RNA extraction. The expression levels of the selected lncRNAs and hsa-miR-107 were measured using quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that individuals with T2DM had higher levels of the lncRNA MIR503HGand TTN-AS1, while the expression of FEZF1-AS1 was lower than in the control group. Furthermore, when T2DM patients were compared to healthy individuals, there was a significant upregulation of the hsa-miR-107transcript. In conclusion, our data implicate the importance of MIR503HG, TTN-AS1, FEZF1-AS1 and hsa-miR-107 expression in T2DM which might yield insight into the complex mechanisms underlying the T2DM pathogenesis.