The Impact of Metallic Nanoparticles on Stem Cell Proliferation and Differentiation

Dayem, Ahmed Abdal; Lee, Soo Bin; Cho, Ssang-Goo

doi:10.3390/nano8100761

Cited by 72 publications

(42 citation statements)

References 185 publications

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“…However, in the plasma group a lower quantity of NPs was seen. Dayem et al stated that exocytosis or the release of NPs occurs through vesicle-dependent release, non-vesicle-dependent release and lysosomal secretion [16]. It is obvious that there must be a correlation between toxicity of particles and their intracellular retention.…”

Section: Discussionmentioning

confidence: 99%

Cultivation of hMSCs in Human Plasma Prevents the Cytotoxic and Genotoxic Potential of ZnO-NP In Vitro

et al. 2019

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Zinc oxide nanoparticles (ZnO-NPs) are commonly used for industrial applications. Consequently, there is increasing exposure of humans to them. The in vitro analysis of cytotoxicity and genotoxicity is commonly performed under standard cell culture conditions. Thus, the question arises of how the results of genotoxicity and cytotoxicity experiments would alter if human plasma was used instead of cell culture medium containing of fetal calf serum (FCS). Human mesenchymal stem cells (hMSCs) were cultured in human plasma and exposed to ZnO-NPs. A cultivation in expansion medium made of DMEM consisting 10% FCS (DMEM-EM) served as control. Genotoxic and cytotoxic effects were evaluated with the comet and MTT assay, respectively. hMSC differentiation capacity and ZnO-NP disposition were evaluated by histology and transmission electron microscopy (TEM). The protein concentration and the amount of soluble Zn 2+ were measured. The cultivation of hMSCs in plasma leads to an attenuation of genotoxic and cytotoxic effects of ZnO-NPs compared to control. The differentiation capacity of hMSCs was not altered. The TEM showed ZnO-NP persistence in cytoplasm in both groups. The concentrations of protein and Zn 2+ were higher in plasma than in DMEM-EM. In conclusion, the cultivation of hMSCs in plasma compared to DMEM-EM leads to an attenuation of cytotoxicity and genotoxicity in vitro.

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Section: Discussionmentioning

confidence: 99%

Cultivation of hMSCs in Human Plasma Prevents the Cytotoxic and Genotoxic Potential of ZnO-NP In Vitro

et al. 2019

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“…Improvement of the efficiency of stem-cell culture system through nanomaterials can be carried out by various methods such as direct addition of NMs to the culture media, coating of culture container and also conjugation of NMs with specific scaffold for 3D culture systems. Nanomaterials will exhibit various aspects of interaction with membrane or intracellular constituents of stem cells which consequently the internalized NPs will modify the cellular signaling pathways [22,[47][48][49]. Here we will discuss about how the marriage of stem-cell therapy with nanotechnology approaches, e.g., application of nanoparticles (NPs) or nanoengineered compounds, can help in overcoming these obstacles to allow clinical application of NSCs therapy.…”

Section: Application Of Nanotechnology In Stem-cell Therapymentioning

confidence: 99%

“…Intriguingly, there are various mechanisms which are involved in the proliferation and differentiation of stem cells via metallic NP-induced procedures, such as modulation of signaling pathways, generation of reactive oxygen species and adjustment of different transcription factors. Metallic NPs and their possible potential of toxicity, in vivo and in vitro have significant effects on stem-cell differentiation and proliferation [22]. Superparamagnetic iron oxide (SPIO) NPs are a type of IONPs (iron oxide NPs) that possess superparamagnetism properties which enable them to migrate to the injured site, so they can be a promising tool for regenerative disease therapy [51].…”

Section: Nanosubstrates For Large Scale Production Of Stem Cellsmentioning

confidence: 99%

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

et al. 2020

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In addition to adverse health outcomes, neurological disorders have serious societal and economic impacts on patients, their family and society as a whole. There is no definite treatment for these disorders, and current available drugs only slow down the progression of the disease. In recent years, application of stem cells has been widely advanced due to their potential of self-renewal and differentiation to different cell types which make them suitable candidates for cell therapy. In particular, this approach offers great opportunities for the treatment of neurodegenerative disorders. However, some major issues related to stem-cell therapy, including their tumorigenicity, viability, safety, metastases, uncontrolled differentiation and possible immune response have limited their application in clinical scales. To address these challenges, a combination of stem-cell therapy with nanotechnology can be a solution. Nanotechnology has the potential of improvement of stem-cell therapy by providing ideal substrates for large scale proliferation of stem cells. Application of nanomaterial in stem-cell culture will be also beneficial to modulation of stem-cell differentiation using nanomedicines. Nanodelivery of functional compounds can enhance the efficiency of neuron therapy by stem cells and development of nanobased techniques for real-time, accurate and long-lasting imaging of stem-cell cycle processes. However, these novel techniques need to be investigated to optimize their efficiency in treatment of neurologic diseases.

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“…SC metallic nanoparticles act as nanobiosensors on functionalization with DNA, peptides, receptors, etc. in biomedical engineering [ 49 ]. In-vivo reconstitution of embryonic SC nanoparticles show therapeutic action for degenerative diseases like Parkinsonism, Alzheimer’s disease, depression, etc.…”

Section: Introductionmentioning

confidence: 99%

Nanoconjugates-Based Stem Cell Therapy for the Management of COVID-19

Desai

Shende

2020

Stem Cell Rev and Rep

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A potential ability of stem cells (SCs) is to regenerate and repair tissues in the human body by providing great prospects for therapeutic applications in the field of medicine. Currently, SC therapy is used in various conditions like diabetes, neurodegenerative disorders, etc. but faces some limitations like patient biocompatibility and chances of cross-infection. SCs are further modulated with nanoconjugates to overcome such challenges and will offer an advantage in the treatment of COVID-19. This pandemic requires design and development of proper treatment to save the life of human beings. Advancements in SC-based nanoconjugated therapy will open new avenues and create a significant impact in the development of futuristic nanomedicine. It may also emerge as a potential therapy for the management of infection in patients suffering from SARS-CoV-2 and related diseases such as pneumonia and virus-induced lung injuries. Graphical abstract Mechanisms of stem cell-based nanoconjugates for inhibition of replication of corona virus.

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The Impact of Metallic Nanoparticles on Stem Cell Proliferation and Differentiation

Cited by 72 publications

References 185 publications

Cultivation of hMSCs in Human Plasma Prevents the Cytotoxic and Genotoxic Potential of ZnO-NP In Vitro

Cultivation of hMSCs in Human Plasma Prevents the Cytotoxic and Genotoxic Potential of ZnO-NP In Vitro

Application of Nanotechnology in Stem-Cell-Based Therapy of Neurodegenerative Diseases

Nanoconjugates-Based Stem Cell Therapy for the Management of COVID-19

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