The Impact of Oral Sodium Chloride Supplementation on Thrive and the Intestinal Microbiome in Neonates With Small Bowel Ostomies: A Prospective Cohort Study

Trautmann, T.; Bang, Corinna; Franke, André; Vincent, Deirdre; Reinshagen, Konrad; Boettcher, Michael

doi:10.3389/fimmu.2020.01421

Cited by 17 publications

(11 citation statements)

References 36 publications

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“…The microbiota analysis was carried out at the Institute of Clinical Molecular Biology in Kiel, Germany. DNA was extracted and purified using the QIAamp fast DNA stool minikit automated on the QIAcube (Qiagen, Hilden, Germany) as described by Trautmann et al ( 62 ).…”

Section: Methodsmentioning

confidence: 99%

Dysbiosis in the Gut Microbiota in Patients with Inflammatory Bowel Disease during Remission

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Dysbiosis in the Gut Microbiota in Patients with Inflammatory Bowel Disease during Remission

et al. 2022

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“…Amplicon sequencing of variable regions 1 and 2 of the 16S rRNA gene was done as been described in detail earlier 24 .…”

Section: Methodsmentioning

confidence: 99%

B-cell-depletion reverses dysbiosis of the microbiome in multiple sclerosis patients

Troci

Zimmermann

Esser

et al. 2022

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To elucidate cross-sectional patterns and longitudinal changes of oral and stool microbiota in multiple sclerosis (MS) patients and the effect of B-cell depletion. We conducted an observational, longitudinal clinical cohort study analysing four timepoints over 12 months in 36 MS patients, of whom 22 initiated B-cell depleting therapy with ocrelizumab and a healthy control group. For microbiota analysis of the oral cavity and the gut, provided stool and oral swab samples underwent 16S rDNA sequencing and subsequent bioinformatic analyses. Oral microbiota-patterns exhibited a reduced alpha-diversity and unique differential microbiota changes compared to stool such as increased levels of Proteobacteria and decreased abundance of Actinobacteria. Following B-cell depletion, we observed increased alpha-diversity in the gut and the oral cavity as well as a long-term sustained reduction of pro-inflammatory Gram-negative bacteria (e.g., Escherichia/Shigella). MS patients have altered stool and oral microbiota diversity patterns compared to healthy controls, which are most pronounced in patients with higher disease activity and disability. Therapeutic B-cell depletion is associated with persisting regression of these changes. Whether these microbial changes are unspecific side-effects of B-cell depletion or indirectly modulate MS disease activity and progression is currently unknown and necessitates further investigations.

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“…Provided stool samples were processed subsequently as has been described in detail earlier [ 42 ]. Briefly, after bead beating of samples, DNA was extracted using the QIAamp DNA fast stool mini kit automated on the QIAcube (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Short-term physical exercise impacts on the human holobiont obtained by a randomised intervention study

et al. 2021

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Background Human well-being has been linked to the composition and functional capacity of the intestinal microbiota. As regular exercise is known to improve human health, it is not surprising that exercise was previously described to positively modulate the gut microbiota, too. However, most previous studies mainly focused on either elite athletes or animal models. Thus, we conducted a randomised intervention study that focused on the effects of different types of training (endurance and strength) in previously physically inactive, healthy adults in comparison to controls that did not perform regular exercise. Overall study duration was ten weeks including six weeks of intervention period. In addition to 16S rRNA gene amplicon sequencing of longitudinally sampled faecal material of participants (six time points), detailed body composition measurements and analysis of blood samples (at baseline and after the intervention) were performed to obtain overall physiological changes within the intervention period. Activity tracker devices (wrist-band wearables) provided activity status and sleeping patterns of participants as well as exercise intensity and heart measurements. Results Different biometric responses between endurance and strength activities were identified, such as a significant increase of lymphocytes and decrease of mean corpuscular haemoglobin concentration (MCHC) only within the strength intervention group. In the endurance group, we observed a significant reduction in hip circumference and an increase in physical working capacity (PWC). Though a large variation of microbiota changes were observed between individuals of the same group, we did not find specific collective alterations in the endurance nor the strength groups, arguing for microbiome variations specific to individuals, and therefore, were not captured in our analysis. Conclusions We could show that different types of exercise have distinct but moderate effects on the overall physiology of humans and very distinct microbial changes in the gut. The observed overall changes during the intervention highlight the importance of physical activity on well-being. Future studies should investigate the effect of exercise on a longer timescale, investigate different training intensities and consider high-resolution shotgun metagenomics technology. Trial registration DRKS, DRKS00015873. Registered 12 December 2018; Retrospectively registered.

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The Impact of Oral Sodium Chloride Supplementation on Thrive and the Intestinal Microbiome in Neonates With Small Bowel Ostomies: A Prospective Cohort Study

Cited by 17 publications

References 36 publications

Dysbiosis in the Gut Microbiota in Patients with Inflammatory Bowel Disease during Remission

Dysbiosis in the Gut Microbiota in Patients with Inflammatory Bowel Disease during Remission

B-cell-depletion reverses dysbiosis of the microbiome in multiple sclerosis patients

Short-term physical exercise impacts on the human holobiont obtained by a randomised intervention study

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