The impact of oxidative stress in reducing the reproductive capacity of the boar-inseminators

Koshevoy, V. I.; Naumenko, S. V.

doi:10.31890/vttp.2020.05.43

Cited by 3 publications

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“…The SOD/CAT and GSH-Px/GSH-Rd indexes prove the preservation of balance in the antioxidant system. In case of decreased reproductive capacity under the influence of oxidative stress deterioration of sperm quality, in particular sperm motility and the number of motile sperm in ejaculate, as a consequence of toxic effects of peroxidation products and increased content of stable metabolites of the nitrogen oxide cycle (Koshevoy & Naumenko, 2020) were observed. In this case, the lack of physiological activity of antioxidant enzymes will have a negative impact on the fertility…”

Section: Discussionmentioning

confidence: 99%

“…In boars of the control group, all the determined parameters of the ejaculate quality were in reference range. In the experimental group of animals (experiment) sperm quality was reduced, especially in terms of sperm motility and the motile sperm count in the ejaculate with a significantly higher content of OS markers in males blood serum (Koshevoy & Naumenko, 2020). Blood samples for biochemical studies were taken after ejaculate was obtained.…”

Section: Methodsmentioning

confidence: 99%

“…It was found that in breeding boars a decrease in reproductive capacity (RC) is characterized by deterioration of sperm quality especially sperm motility and the motile sperm count in the ejaculate and is accompanied by the development of OS due to accumulation of lipid peroxidation (LPO) products and increased content of stable metabolites (Koshevoy & Naumenko, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

Koshevoy¹,

Naumenko²

2020

Theor. Appl. Vet. Med.

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It was found that enzymatic system activity of the antioxidant protection of the breeding boars organism with a decrease in their reproductive ability under oxidative stress was significantly decreased, which was noted by a decrease in the antioxidant capacity. Males of the experimental group had a decrease in superoxide dismutase activity by 30,2% (p ˂ 0,01). At the same time, in the erythrocytes of breeding boars, catalase activity was reduced by 33,9% (p ˂ 0,001) compared with the indicators of the control group animals, due to the high sensitivity to nitric oxide, the content of which was significantly increased during oxidative stress; interacting with nitric oxide, a suppressed form of catalase – ferricatalase-NO was formed. A decrease in the activity of these enzymes is characteristic of the oxidative stress development – which is due to the high level of peroxidation processes in the body of breeding boars. With a decrease in the activity of glutathione system components, an imbalance of the prooxidant-antioxidant system was noted. According to the research results, a decrease in the activity of the glutathione redox cycle enzymes – glutathione peroxidase and glutathione reductase was noted, which in boars with a decrease of reproductive ability were also reduced, and this also confirmed the state of oxidative stress. Thus, boars of the experimental group had a pronounced decrease in glutathione peroxidase activity by 28,4% (p ˂ 0,05), while the activity of glutathione reductase in erythrocytes was significantly reduced by 20,2% (p ˂ 0,05). The lack of physiological activity of antioxidant enzymes will have a negative impact on the fertilizing ability of sperm, because the lack of components of the enzymatic antioxidant defence system affects the activity of enzymes in sperm received from the breeding boars. A balance was observed between the components of the enzymatic antioxidant defense: the ratio of antioxidant enzymes’ activity in the male experimental group was lower than the values in the control group, however, the indices of superoxide dismutase/catalase and glutathione peroxidase/glutathione reductase had the same values, indicating that the balance in the enzymatic system of antioxidant defense was maintained. A decrease in enzyme indices in the experimental group indicated a decrease in the antioxidant potential and confirmed a certain state of oxidative stress in the breeding boars organism.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

Koshevoy¹,

Naumenko²

2020

Theor. Appl. Vet. Med.

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The Use of Gadolinium Orthovanadate Nanoparticles for the Correction of Reproductive Ability in Boars Under Oxidative Stress

Koshevoy

Naumenko

Klochkov

et al. 2021

Ukrainian journal of veterinary sciences

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The search for effec ve and safe means for correc ng male hypofer lity is an urgent problem of modern reproductology. Researchers have proved the possibility of the use of nanopar cles based on oxides of rare-earth elements for the treatment of disorders of male reproduc ve func on. We inves gated the effec veness of the use of gadolinium orthovanadate nanopar cles ac vated by europium, the size of which was 25×8 nm in the dose of 0.0125 mg per kg of live weight to correct reproduc ve ability decrease in boars under oxida ve stress. A er 14 days of hydrosol nanopar cles administra on, we experimentally established the dynamics of the content of oxida ve stress markers and stable metabolites in the nitrogen oxide cycle, and also determined the changes in sperm quality indicators. Thus, conjugated dienes concentra on in the blood serum of boars on the 15th day tended to decrease, and on the 30th day of the study was lower by 9.4% compared with the group of animals before administra on. At the same me, the amount of thiobarbituric acid reac ve substances decreased on the 15th day of the study by 24.7%, and the 30th day -by 48.2%, which indicates the normaliza on of oxida ve processes in males. We noted posi ve changes in the system

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Male Infertility: Pathogenetic Significance of Oxidative Stress and Antioxidant Defence (Review)

Koshevoy¹,

Naumenko²,

Skliarov

et al. 2021

Scientific Horizons

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The basis of the pathogenesis of male infertility is the processes of peroxide oxidation of biological substrates, especially lipids and proteins. By destroying the sperm membrane, toxic peroxidation products reduce its motility and ability to fertilize the egg, which is determined by a decrease in the number of motile sperm in the ejaculate. These changes lead to complete or partial male infertility. The authors of the review found that is accompanied by a damaging effect on the structural and functional activity of the gonads and is manifested, in particular, by an imbalance in the hormonal background of the male body. Similar effects are characteristic of an increase in the content of reactive Nitrogen species and its metabolites, which cause nitrosative stress, which is also the cause of male hypofertility and is inseparable from the state of oxidative stress. In scientific work it is determined that the accumulation of harmful peroxidation products leads to damage and destruction of sperm DNA, reduced activity of acrosomal enzymes and mitochondrial potential of sperm, reduced overall antioxidant activity. This makes it impossible for an adequate response of the body. Multi component antioxidant defense system resists stress. It is represented by enzymatic and non-enzymatic links, which can neutralize harmful radicals and peroxidation products. It contributes to the full manifestation of reproductive function. The presence of powerful antioxidant properties of catalase, superoxide dismutase, and enzymes of the thiol-disulfide system, which form the enzymatic system of antioxidant protection, as well as selenium, zinc, copper, other trace elements, retinol, tocopherol, ascorbic acid, and vitamins as parts of the non-enzymatic system is shown. The efficiency of registration is substantiated thin biochemical shift detectors or complex methods, such as total antioxidant status of sperm or sperm plasma, mitochondrial membrane potential, etc along with simple markers of oxidative stress, such as diene conjugates, malonic dialdehyde, and metabolites of the Nitrogen Oxide cycle. Given the leading role of oxidative stress in the development of male hypofertility, the prospect of further research is the search for modern means for correction, especially among substances with pronounced redox activity

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The impact of oxidative stress in reducing the reproductive capacity of the boar-inseminators

Cited by 3 publications

References 0 publications

The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

The Use of Gadolinium Orthovanadate Nanoparticles for the Correction of Reproductive Ability in Boars Under Oxidative Stress

Male Infertility: Pathogenetic Significance of Oxidative Stress and Antioxidant Defence (Review)

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