The impact of PCR in the management of prosthetic joint infections

Saeed, Kordo; Ahmad-Saeed, Nusreen

doi:10.1586/14737159.2015.1046437

Cited by 11 publications

(4 citation statements)

References 48 publications

(62 reference statements)

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“…Advances in molecular diagnostics have enabled laboratories to use nucleic acid amplification techniques, such as polymerase chain reaction (PCR). This can then accelerate the diagnosis, identify causative agents, and direct targeted antibiotic therapy [6][7][8]. Depending on the assay (8), PCR can detect a large range of microorganisms, including bacteria/yeast that are fastidious or slow growing, or in circumstances in which previous antimicrobial use may lead to a false-negative culture result.…”

Section: Introductionmentioning

confidence: 99%

“…This can then accelerate the diagnosis, identify causative agents, and direct targeted antibiotic therapy [6][7][8]. Depending on the assay (8), PCR can detect a large range of microorganisms, including bacteria/yeast that are fastidious or slow growing, or in circumstances in which previous antimicrobial use may lead to a false-negative culture result. Despite this, multi-plex PCR and/or 16 S rRNA PCR are still not widely available in many diagnostic laboratories routinely [9].…”

Section: Introductionmentioning

confidence: 99%

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A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel

Saeed

Ahmad-Saeed

Annett

et al. 2022

Eur J Clin Microbiol Infect Dis

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Septic arthritis is a serious condition with significant morbidity and mortality, routinely diagnosed using culture. The FDA has recently approved the rapid molecular BioFire® Joint Infection Panel (BJIP) for synovial fluid. We aimed to evaluate the BJIP compared to culture and its potential use in patient management. A multicentre retrospective evaluation of BJIP was conducted in the UK and Ireland. Positive percent agreement (PPA) and negative percent agreement (NPA) were calculated between the BJIP and routine culture. A multidisciplinary team (MDT) discussion addressing the optimal or potential case use of the assay practice was facilitated. Three hundred ninety-nine surplus synovial fluid samples (~ 70% from native joints) from eight centres were processed using BJIP in addition to routine culture. An increased yield of positive results was detected using BJIP compared to routine culture (98 vs 83), giving an overall PPA of 91.6% and overall NPA of 93% for the BJIP compared to culture results. The BJIP detected resistant markers and additional organisms that could influence antibiotic choices including Neisseria gonorrhoeae and Kingella kingae. The MDT agreed that the assay could be used, in addition to standard methods, in adult and children patients with specialist advice use based on local needs. Rapid results from BJIP were assessed as having potential clinical impact on patient management. Organisms not included in the panel may be clinically significant and may limit the value of this test for PJI.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel

Saeed

Ahmad-Saeed

Annett

et al. 2022

Eur J Clin Microbiol Infect Dis

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“…It is exceptionally perceptive when it comes to the detection of the previously specified organisms of interest. In the case of PJIs, usually multiple species are targeted, so broad-range PCR is a more sufficient technique, although specific PCR can be useful if a likely cause of periprosthetic joint infection is known [ 67 ]. A study from 2022 by Prinz et al applied sensitive multiplex RT-qPCR designed to detect Cutibacterium using sonication fluid explanted from patients with PJI as a sample.…”

Section: Molecular Diagnostic Methodsmentioning

confidence: 99%

Modern Microbiological Methods to Detect Biofilm Formation in Orthopedy and Suggestions for Antibiotic Therapy, with Particular Emphasis on Prosthetic Joint Infection (PJI)

Mikziński,

Kraus,

Widelski

et al. 2024

Microorganisms

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Biofilm formation is a serious problem that relatively often causes complications in orthopedic surgery. Biofilm-forming pathogens invade implanted foreign bodies and surrounding tissues. Such a condition, if not limited at the appropriate time, often requires reoperation. This can be partially prevented by selecting an appropriate prosthesis material that prevents the development of biofilm. There are many modern techniques available to detect the formed biofilm. By applying them we can identify and visualize biofilm-forming microorganisms. The most common etiological factors associated with biofilms in orthopedics are: Staphylococcus aureus, coagulase-negative Staphylococci (CoNS), and Enterococcus spp., whereas Gram-negative bacilli and Candida spp. also deserve attention. It seems crucial, for therapeutic success, to eradicate the microorganisms able to form biofilm after the implantation of endoprostheses. Planning the effective targeted antimicrobial treatment of postoperative infections requires accurate identification of the microorganism responsible for the complications of the procedure. The modern microbiological testing techniques described in this article show the diagnostic options that can be followed to enable the implementation of effective treatment.

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“…Most of the gold standard microbial methods for diagnosing PJI are based on Koch’s culturing and colony counting methods and only deliver results after several days . Additionally, culture-based methods are susceptible to contamination, which can lead to false positive diagnoses, and yield false negative diagnoses in up to 30% of all cases .…”

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confidence: 99%

Detection of Prosthetic Joint Infection Based on Magnetically Assisted Surface Enhanced Raman Spectroscopy

et al. 2017

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Accurate and rapid diagnosis of prosthetic joint infection (PJI) is vital for rational and effective therapeutic management of this condition. Several diagnostic strategies have been developed for discriminating between infected and noninfected cases. However, none of them can reliably diagnose the whole spectrum of clinical presentations of PJI. Here, we report a new method for PJI detection based on magnetically assisted surface enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles (MNP@Strep) whose surface is functionalized with suitable biotinylated antibodies and then coated with silver nanoparticles by self-assembly. The high efficiency of this approach is demonstrated by the diagnosis of infections caused by two bacterial species commonly associated with PJI, namely, Staphylococcus aureus and Streptococcus pyogenes. The method's performance was verified with model samples of bacterial lysates and with four real-matrix samples of knee joint fluid spiked with live pathogenic bacterial cells. This procedure is operationally simple, versatile, inexpensive, and quick to perform, making it a potentially attractive alternative to established diagnostic techniques based on Koch's culturing or colony counting methods.

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The impact of PCR in the management of prosthetic joint infections

Cited by 11 publications

References 48 publications

A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel

A multicentre evaluation and expert recommendations of use of the newly developed BioFire Joint Infection polymerase chain reaction panel

Modern Microbiological Methods to Detect Biofilm Formation in Orthopedy and Suggestions for Antibiotic Therapy, with Particular Emphasis on Prosthetic Joint Infection (PJI)

Detection of Prosthetic Joint Infection Based on Magnetically Assisted Surface Enhanced Raman Spectroscopy

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