The impact of plant rolC oncogene on ginsenoside production by ginseng hairy root cultures

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

“…the long-standing test system based on Panax ginsengtransformed cell cultures (Bulgakov et al, 1998) and the recently transformed Arabidopsis (Arabidopsis thaliana) cells. Measurements of ROS in these cultures revealed that ROS inhibition by rolB was also observed in these systems (Table I).…”

“…These lines have been previously characterized to have stable morphology, growth, biosynthetic parameters, and levels of rolB expression . Cell suspensions were cultivated using W B/A liquid medium (Bulgakov et al, 1998) supplemented with 0.5 mg L 21 6-benzylaminopurine and 2.0 mg L 21 a-naphthaleneacetic acid in the dark (excluding experiments with paraquat, where cells were cultivated in the light; see below) at 24°C with 12-d subculture intervals.…”

“…The Panax ginseng GV (vector control) and rolB-transformed callus cultures were cultivated using W 4CPA medium supplemented with 0.4 mg L 21 4-chlorophenoxyacetic acid in the dark as described previously (Bulgakov et al, 1998). The suspension variants of these cultures were grown in W 4CPA liquid medium.…”

“…The calli were cultivated using W 2,4-D medium (Bulgakov et al, 1998) supplemented with 0.4 mg L 21 2,4-dichlorophenoxyacetic acid in the dark at 24°C with 30-d subculture intervals. Two rolB-expressing callus lines (AtB-1 and AtB-2) were selected from these primary calli as described previously to obtain lines with different strengths of rolB expression.…”

“…These cultures were 1 year of age. The cell suspension cultures AtB-1 and AtB-2 were cultivated using W 2,4-D liquid medium (Bulgakov et al, 1998) in the dark at 24°C with 14-d subculture intervals.…”