The impact of plasma triglyceride and apolipoproteins concentrations on high-density lipoprotein subclasses distribution

Abstract

ObjectiveTo investigate the effect of triglyceride (TG) integrates with plasma major components of apolipoproteins in HDL subclasses distribution and further elicited the TG-apolipoproteins (apos) interaction in the processes of high density lipoprotein (HDL) mature metabolic and atherosclerosis related diseases.MethodsContents of plasma HDL subclasses were quantities by two-dimensional gel electrophoresis associated with immunodetection in 500 Chinese subjects.ResultsContents of preβ1-HDL, HDL3a, and apoB-100 level along with apoB-100/A-I ratio were significantly increased, whereas there was a significant reduction in the contents of HDL2, apoA-I level as well as apoC-III/C-II ratio with increased TG concentration. Moreover, preβ1-HDL contents is elevated about 9 mg/L and HDL2b contents can be reduced 21 mg/L for 0.5 mmol/L increment in TG concentration. Moreover, with increase of apoA-I levels, HDL2b contents were marginally elevated in any TG concentration group. Furthermore, despite of in the apoB-100/A-I < 0.9 group, the contents of preβ1-HDL increased, and those of HDL2b decreased significantly for subjects in both high and very high TG levels compared to that in normal TG levels. Similarly, in the apoB-100/A-I ≥ 0.9 group, the distribution of HDL subclasses also showed abnormality for subjects with normal TG levels.ConclusionsThe particle size of HDL subclasses tend to small with TG levels increased which indicated that HDL maturation might be impeded and efficiency of reverse cholesterol transport(RCT) might be weakened. These data suggest that TG levels were not only significantly associated with but liner with the contents of preβ1-HDL and HDL2b. They also raise the possibility that the TG levels effect on HDL maturation metabolism are subjected to plasma apolipoproteins and apolipoproteins ratios.

High-Density Lipoprotein Subfractions - What the Clinicians Need to Know

“…Accordingly, average HDL particle size was reduced in women with CAD compared to healthy subjects [13]; patients with acute ischemic stroke had significantly smaller HDL size, more HDL3 and less HDL2b particles [14], and in myocardial infarction survivors, HDL2b was significantly lower than in controls and inversely correlated with body mass index, smoking, TG and low-density lipoprotein-cholesterol (LDL-C) levels [15]. A similar reduction in large HDL2 particles and an increase in small particles were also observed in non-obese type 2 diabetics [16], in overweight and obese subjects [17,18,19], in subjects with metabolic syndrome [20] and in CHD subjects with diabetes when compared to CHD subjects without diabetes [21]. …”

“…Pre-β-particles, which represent only a small portion of HDL, play a key role in the reverse cholesterol transport process; however, CHD patients show higher levels of pre-β-particles compared to healthy subjects [22,23,24], due to defective activity of enzymes involved in the HDL maturation cycle or to enhanced enzymatic remodelling of α-HDL induced by high plasma TG levels (table 2) [17,25,26]. …”

Cinética plasmática da lipoproteína de baixa densidade e avaliação dos aspectos qualitativos da lipoproteína de alta densidade em indivíduos com artrite reumatóide

“…Muitos estudos prospectivos demonstraram que a HDL pequena -enriquecida de TG -está associada a doenças cardiovasculares. Isso se deve ao fato de que esta HDL sofre maior ação da lipase hepática e da CETP (RYE et al, 1999) tendo sua maturação impedida, dificultando o transporte reverso do colesterol (TIAN et al, 2011).…”

Papel do ômega-3 nas características físico-químicas da HDL e LDL e possível associação com medidas Z-scan em indivíduos adultos

“…de genes e enzimas responsáveis pela síntese de ácidos graxos e TAG, porém existem evidências que os ácidos graxos ômega-3 podem atuar aumentando a expressão do SREBP-2, que modula as enzimas envolvidas na síntese de colesterol (XU et al, 2001;LE JOSSIC-CORCOS et al, 2005;HARRIS e BULCHANDANI, 2006;ARAI et al, 2009). Em modelos animais e in vitro, tanto EPA quanto o DHA diminuíram a atividade do SREBP-1, com consequente diminuição da expressão de enzimas lipogênicas (CAPUTO et al, 2011;HOWELL et al 2009;KAJIKAWA et al, 2009 DREXEL et al, 1992;YANG et al, 2005;XIAN et al, 2009;LAGOS et al, 2009;TIAN et al, 2006;TIAN et al, 2011).…”