The impact of pregnancy on the HIV-1-specific T cell function in infected pregnant women

Hygino, Joana; Vieira, Morgana M.; Kasahara, Taissa M.; Xavier, Luciana F.; Blanco, Bernardo; Guillermo, Landi V. C.; Filho, Renato G.S.; Saramago, Carmen S.M.; Lima-Silva, Agostinho A.; Oliveira, Afonso de Liguori; Guimarães, Vander; Andrade, Arnaldo F.B.; Bento, Cleonice A.M.

doi:10.1016/j.clim.2012.10.001

Cited by 7 publications

(11 citation statements)

References 51 publications

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“…Women who reported using ART before pregnancy were those with the highest IFN-γ levels. Our data are thus consistent with an earlier study showing expansion of IFN-γ secreting HIV-specific T cells in HIVinfected individuals 24 including those under ART. 28 Altogether, our data suggest that two mechanisms could participate in ART efficacy for reducing maternal-HIV transmission: the direct impact of ART on HIV replication and the increased IFN-γ production during pregnancy.…”

Section: Discussionsupporting

confidence: 83%

“…The TNF-α has been shown, along with IL-8, to be involved in viral transplacental transmission, 33 and, perhaps most importantly, HIV replication during P. falciparum infection has been reported to be mediated by TNF-alpha; 34 The significantly lower plasma level of TNF-α found in our study at delivery compared with inclusion may reflect 1) the direct influence of IL-10 on this cytokine 24,35 or 2) the markedly lower overall prevalence of plasmodial infection, or 3) the decreased immune activation associated with institution of ART.…”

Section: Discussioncontrasting

confidence: 45%

“…11,22,23 The data we present here suggest that even in immunosuppressed women P. falciparum infection is associated with an elevated plasma IL-10 concentration, perhaps yet another indication of the essential anti-inflammatory role this cytokine plays in the context of maintenance of the pregnancy when exposed to inflammatory insults. Parasite-induced IL-10 may also contribute, by suppression of inflammatory activity, to reducing HIV viral load, 24,25 with possibly a further indirect effect on viral replication. 26 The absence of an association between plasma IL-10 and infection with P. falciparum at delivery may reflect the longevity of the infections in the sense that those present at delivery were likely-given the fact that all women were subjected to malaria chemoprevention-to have been recently acquired and therefore to have had comparatively little time to induce immunological alterations detectable at the systemic level.…”

Section: Discussionmentioning

confidence: 99%

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Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Ibitokou¹,

Denoeud-Ndam²,

Ezinmègnon³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. We investigated the circulating plasma levels of Th1-(Interleukin-2 [IL-2], tumor necrosis factor-α [TNF-α], interferon-gamma [IFN-γ]) and Th2-type (IL-4, IL-5, IL-10) cytokines in human immunodeficiency virus (HIV)-infected pregnant women living in a malaria-endemic area. We analyzed samples from 200 pregnant women included in the prevention of pregnancy-associated malaria in HIV-infected women: cotrimoxazole prophylaxis versus mefloquine (PACOME) clinical trial who were followed until delivery. Cytokine concentrations were measured by flow cytometrybased multiplex bead array. Significantly elevated levels of IL-10 and lower levels of TNF-α were observed at delivery compared with inclusion (P = 0.005). At inclusion, the presence of circulating IFN-γ, a higher CD4 + T cell count and having initiated intermittent preventive treatment of malaria with sulfadoxine pyrimethamine (SP-IPTp) were all associated with a lower likelihood of Plasmodium falciparum infection. At delivery, the inverse relationship between the presence of infection and circulating IFN-γ persisted, although there was a positive association between the likelihood of infection and the presence of circulating TNF-α. Initiation of antiretroviral therapy was associated with elevated IL-5 production. Consistent with our own and others' observations in HIV seronegative subjects, this study shows circulating IL-10 to be a marker of infection with P. falciparum during pregnancy even in HIV-infected women, although plasma IFN-γ may be a marker of anti-malarial protection in such women.

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Section: Discussionsupporting

confidence: 83%

Section: Discussioncontrasting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

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Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Ibitokou¹,

Denoeud-Ndam²,

Ezinmègnon³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…HIV-1 positive non-pregnant (PnP) and pregnant (PP) women were recruited from Chelsea and Westminster Hospital and St. Mary's Hospital, with any participant who delivered preterm excluded from analysis. Where possible blood was sampled at each trimester and delivery; two first trimester (<13 weeks gestation), 15 second trimester (13-27 weeks), 31 third trimester (>28 weeks) and 9 delivery samples were collected (median and range gestation were 8 (8), 22 (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27), 30 (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39), and 40 (37-40) respectively). Samples were processed within 6 h of collection, with peripheral blood mononuclear cells (PBMC) isolated in a containment level 3 laboratory.…”

Section: Study Design Ethics Setting and Participantsmentioning

confidence: 99%

“…Through anti-CD3 stimulation of peripheral leukocytes pregnant HIV-1 positive women have been shown to have higher IL-10 and lower TNFα and IFNγ responses than non-pregnant HIV-1 positive women (32). Physiological changes occurring during pregnancy are also known to affect ART pharmacokinetics (33).…”

Section: Introductionmentioning

confidence: 99%

Pregnancy Gestation Impacts on HIV-1-Specific Granzyme B Response and Central Memory CD4 T Cells

et al. 2020

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Pregnancy induces alterations in peripheral T-cell populations with both changes in subset frequencies and anti-viral responses found to alter with gestation. In HIV-1 positive women anti-HIV-1 responses are associated with transmission risk, however detailed investigation into both HIV-1-specific memory responses associated with HIV-1 control and T-cell subset changes during pregnancy have not been undertaken. In this study we aimed to define pregnancy and gestation related changes to HIV-1-specific responses and T-cell phenotype in ART treated HIV-1 positive pregnant women. Eleven non-pregnant and 24 pregnant HIV-1 positive women were recruited, peripheral blood samples taken, fresh cells isolated, and compared using ELISpot assays and flow cytometry analysis. Clinical data were collected as part of standard care, and non-parametric statistics used. Alterations in induced IFNγ, IL-2, IL-10, and granzyme B secretion by peripheral blood mononuclear cells in response to HIV-1 Gag and Nef peptide pools and changes in T-cell subsets between pregnant and non-pregnant women were assessed, with data correlated with participant clinical parameters and longitudinal analysis performed. Cross-sectional comparison identified decreased IL-10 Nef response in HIV-1 positive pregnant women compared to non-pregnant, while correlations exhibited reversed Gag and Nef cytokine and protease response associations between groups. Longitudinal analysis of pregnant participants demonstrated transient increases in Gag granzyme B response and in the central memory CD4 T-cell subset frequency during their second trimester, with a decrease in CD4 effector memory T cells from their second to third trimester. Gag and Nef HIV-1-specific responses diverge with pregnancy time-point, coinciding with relevant T-cell phenotype, and gestation associated immunological adaptations. Decreased IL-10 Nef and both increased granzyme B Gag response and central memory CD4 T cells implies that amplified antigen production is occurring, which suggests a period of compromised HIV-1 control in pregnancy.

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Immunogenicity of Hepatitis B Vaccine in HIV Exposed Uninfected Infants

et al. 2015

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There is paucity of knowledge about the immunogenicity of vaccines in infants who have been exposed to HIV in-utero but have remained uninfected. The authors studied the immunogenicity of 3 doses of recombinant hepatitis B vaccine at 6,10,14 wk of age in HIV exposed but uninfected (HEU) infants. After 3 mo of last dose of the vaccine, out of 26 infants, 23 (89.5 %) infants were found to be responders (Anti HBs IgG titres ≥ 10 mIU/ml) and 3 (11.5 %) babies remained non responders (Anti HBs IgG titres < 10 mIU/ml). The proportion of babies who were non responders were higher when compared to similar studies done on unexposed and uninfected infants, suggesting a poorer immunogenicity of hepatitis B vaccine in these infants.

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The impact of pregnancy on the HIV-1-specific T cell function in infected pregnant women

Cited by 7 publications

References 51 publications

Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Pregnancy Gestation Impacts on HIV-1-Specific Granzyme B Response and Central Memory CD4 T Cells

Immunogenicity of Hepatitis B Vaccine in HIV Exposed Uninfected Infants

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