The impact of pronuclei morphology and dynamicity on live birth outcome after time-lapse culture

Azzarello, A.; Hoest, T.; Mikkelsen, Anne Lis

doi:10.1093/humrep/des210

Cited by 142 publications

(95 citation statements)

References 31 publications

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“…Our results were comparable with those given in the literature [11,18]. It makes us presume that Esmya does not have adverse effect on the quality of egg cell produced.…”

Section: Case Reportsupporting

confidence: 91%

Pre-treatment with ulipristal acetate before ICSI procedure: a

Wdowiak¹

2013

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SummaryEpidemiologic data indicate that infertility is a common global problem being diagnosed in approximately one sixth of all couples. One of the reasons responsible for the inability to conceive is the presence of uterine fibroids. Many reports published recently have emphasized the high efficiency of ulipristal acetate in the treatment of uterine fibroids. In June 2012, a 35-year-old woman reported to the Non-Public Health Care Unit "Ovum Reproduction and Andrology" in Lublin for continued treatment of infertility.However, ultrasound performed on the day of the visit at Ovum showed two intramural uterine fibroids: one of them, size 2.26 cm × 2.53 cm, was located in the front uterine wall touching the endometrium and the other one with a diameter of 1.2 cm was located in the posterior uterine wall, not compressing the endometrium. Taking all of this into consideration along with the treatment conducted so far, the couple has been qualified for an ICSI (intracytoplasmic sperm injection) procedure. Due to the presence of the fibroid in the front wall of the uterus, which might have adversely affected embryo implantation in the previous IVF procedures, an attempt was made to reduce the fibroid volume with 3-month treatment with Esmya 5 mg 1 × 1 given for 84 days. Ultrasound performed after discontinuation of Esmya treatment showed normal size of the uterus with smooth contours and the previously detected intramural fibroid located in the posterior wall, was found to have a diameter of 1.08 cm, illustrating a reduction from pre-treatment size. The fibroid located in the front wall of the uterus previously detected by ultrasound was no longer visible. The patient underwent ICSI treatment. The risk of multiple pregnancies was reduced by an appropriate selection of a single blastocyst for transfer. Pregnancy was achieved and further ultrasound confirmed normal anatomical structure of the fetus and the absence of other fibroids.Key words: fibroid, infertility, ulipristal acetate, ICSI. StreszczenieDane epidemiologiczne wskazują, że niepłodność jest powszechnym problemem w skali światowej, bowiem dotyczy jednej szóstej wszystkich par. Jedną z przyczyn niemożności zajścia w ciążę jest mięśniakowatość macicy. Liczne publikacje z ostatnich lat podkreślają wysoką skuteczność octanu uliprystalu w terapii mięśniaków macicy. Pacjentka, lat 35, zgłosiła się w czerwcu 2012 r. w celu kontynuacji leczenia niepłodności do ośrodka Ovum. Na podstawie badania ultrasonograficznego (USG) przeprowadzonego w dniu zgłoszenia się pacjentki, uwidoczniono obecność dwóch mięśniaków macicy zlokalizowanych śródściennie: w przedniej ścianie macicy mięśniak o wymiarach 2,26 cm × 2,53 cm w styczności z endometrium oraz w tylnej ścianie macicy -średnica 1,2 cm bez ucisku na endometrium. Ze względu na dotychczasowy przebieg leczenia parę zakwalifikowano do procedury mikroiniekcji plemnika do komórki jajowej (intracytoplasmic sperm injection -ICSI). Przed programem podjęto próbę redukcji objętości mięśniaka, stosując trzymiesięczną terapię preparat...

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“…Our results were comparable with those given in the literature [11,18]. It makes us presume that Esmya does not have adverse effect on the quality of egg cell produced.…”

Section: Case Reportsupporting

confidence: 91%

Pre-treatment with ulipristal acetate before ICSI procedure: a

Wdowiak¹

2013

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“…Similar reports have linked with high sensitivity and specificity the use of TLM with the ability of prediction of the cleavage stage embryos and their potential to reach the blastocyst stage, resulting in increased live births [12,16,19,26,31,34,39,41]. On the other hand, other reports showed that TLM parameters are not able to predict live birth when compared with the conventional methods [33,34,42].…”

Section: Discussionmentioning

confidence: 70%

Morphokinetic parameters of early embryo development via time lapse monitoring and their effect on embryo selection and ICSI outcomes: a prospective cohort study

Siristatidis

Komitopoulou

Makris

et al. 2015

J Assist Reprod Genet

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Purpose To compare the outcomes of embryos selected via time lapse monitoring (TLM) versus those selected with conventional methods of selection in subfertile women undergoing ICSI. Methods The study population (239 women) was classified into two groups, based on the monitoring method used: Group 1 (TLM) and Group 2 (conventional monitoring). Groups were compared according to the clinical and ICSI cycle characteristics and reproductive outcomes, while transfers were performed at day 2 or 3. Subgroup analyses were performed, in women of both groups according to age and clinical parameters, and in embryos of Group 1 based on their cellular events.Results There was a statistically significant difference between the two study groups with regard to the outcome parameters, favoring Group 1 and especially in women >40 years of age. No differences were found in subgroup analyses in participants of both groups, regarding the stimulation protocol used, number of the oocytes retrieved and type of subfertility, while in Group 1 the percentages of "in range" cellular events were higher in certain divisions in ages 35-40, non-smokers, and the GnRH-agonist group, and in embryos that resulted in pregnancy. Conclusion Morphokinetic parameters of early embryo development via TLM are related to the characteristics of subfertile patients and associated with ICSI outcomes.

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“…In the present study, the pronuclear morphology was evaluated at 16-18 h after ICSI. Recent data using time-lapse imaging demonstrated that the pronuclear morphology changes over time, indicating that the single light microscopy observation approach is deficient in comparison to time-lapse [27]. Therefore, our findings must be confirmed by future trials, if possible using time-lapse imaging technology.…”

Section: Discussionmentioning

confidence: 70%

“…Previous time-lapse observations evaluating the pronuclear morphology [27], suggests that the number and distribution of NPBs does not predict the quality of the zygote, in terms of live birth potential. Despite this, these morphological features have been chosen as a quality indicator of the fertilized oocyte.…”

Section: Discussionmentioning

confidence: 91%

The combination of pronuclear and blastocyst morphology: a strong prognostic tool for implantation potential

Braga

Setti

Figueira

et al. 2013

J Assist Reprod Genet

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Purpose To (i) investigate a possible association between different features of pronuclear (PN) morphology and different features of blastocyst morphology, (ii) evaluate the combination of PN and blastocyst morphologies as a predictive factor for ICSI outcomes and (iii) identify possible contributing factors to poor PN morphology. Methods This study included 908 normally fertilised zygotes reaching full blastocyst stage, obtained from 350 patients undergoing ICSI cycles, in which the implantations rates were 0 % or 100 %. The influence of PN morphology on blastocyst morphology and on the rates of pregnancy and miscarriage was investigated. Embryos were graded and split into three groups, taking into consideration both the PN and the blastocyst status. The pregnancy rate was compared among these groups. Results Inner cell mass (ICM) alterations were correlated with the number of nucleolar precursor bodies (NPB), while trophectoderm alterations were correlated with the size of the pronuclei and the distribution of the NPB. The distribution of the NPB had an impact on the chances of pregnancy. A significant difference was observed among the groups regarding the pregnancy rate. The maternal age, number of aspirated follicles and number of retrieved oocytes influenced the incidence of PN defects. Conclusions These findings suggest that a lower oocyte yield may lead to higher-quality PN zygotes. In addition, different PN features may influence further embryo development, especially the quality of the blastocyst. Moreover, the association between PN and blastocyst morphology may be used as a prognostic tool for implantation.

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The impact of pronuclei morphology and dynamicity on live birth outcome after time-lapse culture

Cited by 142 publications

References 31 publications

Pre-treatment with ulipristal acetate before ICSI procedure: a

Pre-treatment with ulipristal acetate before ICSI procedure: a

Morphokinetic parameters of early embryo development via time lapse monitoring and their effect on embryo selection and ICSI outcomes: a prospective cohort study

The combination of pronuclear and blastocyst morphology: a strong prognostic tool for implantation potential

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