The Impact of Quorum Sensing on the Modulation of Phage-Host Interactions

León‐Félix, Josefina; Villicaña, Claudia

doi:10.1128/jb.00687-20

Cited by 25 publications

(11 citation statements)

References 141 publications

(188 reference statements)

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“…Certain C. difficile phage appear to bear and disseminate agr variants amongst their hosts, highlighting the complexity of microbiome dynamics and the difficulty in limiting investigations to single kingdoms (Hargreaves et al, 2014). This also supports the recent implication of bacterial QS as a broad mediator of phage-host interactions in the gut (León-Félix and Villicaña, 2021). Such insights certainly require additional scrutiny, but could prove significant in the development of phage therapy.…”

Section: Qs Influence On Collective Bacterial Behaviours In the Gutmentioning

confidence: 65%

Quorum sensing in human gut and food microbiomes: Significance and potential for therapeutic targeting

Falà

Álvarez‐Ordóñez²,

Filloux³

et al. 2022

Front. Microbiol.

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Human gut and food microbiomes interact during digestion. The outcome of these interactions influences the taxonomical composition and functional capacity of the resident human gut microbiome, with potential consequential impacts on health and disease. Microbe-microbe interactions between the resident and introduced microbiomes, which likely influence host colonisation, are orchestrated by environmental conditions, elements of the food matrix, host-associated factors as well as social cues from other microorganisms. Quorum sensing is one example of a social cue that allows bacterial communities to regulate genetic expression based on their respective population density and has emerged as an attractive target for therapeutic intervention. By interfering with bacterial quorum sensing, for instance, enzymatic degradation of signalling molecules (quorum quenching) or the application of quorum sensing inhibitory compounds, it may be possible to modulate the microbial composition of communities of interest without incurring negative effects associated with traditional antimicrobial approaches. In this review, we summarise and critically discuss the literature relating to quorum sensing from the perspective of the interactions between the food and human gut microbiome, providing a general overview of the current understanding of the prevalence and influence of quorum sensing in this context, and assessing the potential for therapeutic targeting of quorum sensing mechanisms.

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Section: Qs Influence On Collective Bacterial Behaviours In the Gutmentioning

confidence: 65%

Quorum sensing in human gut and food microbiomes: Significance and potential for therapeutic targeting

Falà

Álvarez‐Ordóñez²,

Filloux³

et al. 2022

Front. Microbiol.

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“…We also tested whether ST14 could sense the presence of competition, for instance by quorum sensing mechanisms, and induce prophages and the production viral particles 38 . Our results show that growth of ST14 with spent supernatant of BJ1 did not result in increased viral release (Figure S1CDE).…”

Section: Resultsmentioning

confidence: 99%

Competition between phage-resistance mechanisms determines the outcome of bacterial co-existence

Rendueles

Sousa

Rocha

2022

Preprint

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Many bacterial species carry multiple phages, and despite the potential cost, which includes cell death upon phage induction, prophages can also provide the host multiple fitness advantages, including infection of direct competitors. However, the long-term efficiency of displacing conspecifics through prophage induction has received little attention. We experimentally coevolved a polylysogenic Klebsiella pneumoniae strain with a phage-sensitive strain, BJ1, under different degrees of phage pressure. We then followed the adaptation process and the emergence of resistance. After 30 days, population yield remained stable, and although BJ1 was present in all conditions, its frequency was higher when phage pressure was stronger. Resistance to phages emerged fast through mutations interrupting the capsule biosynthesis. In contrast to our expectation, lysogenic conversion was rare and costly because new BJ1 lysogens exhibited exacerbated death rates and were easily outcompeted. Unexpectedly, the adaptation process changed at longer time scales, where BJ1 populations adapted by fine-tuning the production of capsule, reducing the ability of phage to absorb, while remaining capsulated. These resistant clones are pan-resistant to a large panel of phages. Most intriguingly, some clones exhibited transient non-genetic resistance to phages. Our experimental and modelling results highlight the diversity, dynamics and competition between phage-resistance mechanisms during coevolution and how these are driven by phage density.

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“…Despite significant progress in recognizing the critical role of QS signaling in phage-bacterium interactions, studies on the biological significance of QS are still in their infancy [35]. Unveiling novel antiphage mechanisms governed by QS will have implications for understanding the relative importance of QS as a driver of ecological processes in complex ecosystems modulated by phage-host.…”

Section: Discussionmentioning

confidence: 99%

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

et al. 2021

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In response to the restriction of nutrients and predation by natural enemies, bacteria have evolved complex coping strategies to ensure the reproduction and survival of individual species. Quorum sensing (QS) is involved in the bacterial response to phage predation and regulation of cellular metabolism. However, to date, no clear evidence exists regarding the involvement of autoinducer‐2 (AI‐2)‐mediated QS systems in Escherichia coli in response to the challenges of nutrient restriction and phage infection. In this study, the role of the AI‐2‐mediated QS system in resisting T4 phage infection and regulating cell mechanisms in E. coli was revealed for the first time. This effect of the AI‐2‐mediated QS was achieved by simultaneously downregulating the T4 absorption site and carbon and glucose metabolism. Additionally, we found that lsrB, a metabolic brake, participates in AI‐2‐mediated regulation and maintenance of the normal metabolic balance of cells. The novel phage defense strategy and regulation and maintenance of cellular metabolism effectively limited the expansion of the phage population.

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The Impact of Quorum Sensing on the Modulation of Phage-Host Interactions

Cited by 25 publications

References 141 publications

Quorum sensing in human gut and food microbiomes: Significance and potential for therapeutic targeting

Quorum sensing in human gut and food microbiomes: Significance and potential for therapeutic targeting

Competition between phage-resistance mechanisms determines the outcome of bacterial co-existence

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

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