The impact of short-lasting repeated vibrations on retrograde axonal transport, the expression of CGRP and parvalbumin in lower lumbar dorsal root ganglia

Pavel, Jaroslav; Hricová, Ľudmila; Jergová, Stanislava; Lukáčová, Nadežda

doi:10.1016/j.brainres.2011.04.023

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Only DRG neuronal cells showing cytoplasmic staining and a clear nucleus were counted in order to minimize overcounting of labeled primary sensory neurons. The number of small-and large-sized immunoreactive cells per section was manually counted using ImageJ software (NIH, Bethesda, USA) and expressed as percentage of the total number of small-sized or large-sized primary sensory neurons as described previously (Pavel et al 2011). To avoid interassay variability, the sections from control tissue underwent an immunohistochemical staining on the same slide as sections from CCI group.…”

Section: Cell Countingmentioning

confidence: 99%

The Characterization of AT1 Expression in the Dorsal Root Ganglia After Chronic Constriction Injury

et al. 2016

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To clarify the role of Angiotensin II in the regulation of sensory signaling, we characterized the AT expression in neuronal subpopulation of lower lumbar dorsal root ganglia under normal conditions and its alteration in neuropathic pain model. The characterization of AT expression was done under control and after the chronic constriction injury induced by four loose ligatures of the sciatic nerve representing the model of posttraumatic painful peripheral neuropathy. Major Angiotensin II receptor type was expressed in approximately 43 % of small-sized and 62 % of large-sized neurons in control. The AT overexpression after sciatic nerve ligation lasting 7 days was detected predominantly in small-sized AT immunoreactive neurons (about 38 % increase). Chronic constriction injury caused a statistically marked increase in number of the small-sized peptidergic (CGRP immunoreactive) neuronal subpopulation expressing AT (about 64 %). The subpopulations of AT-immunoreactive and nonpeptide-containing primary sensory neurons revealed by IB4 binding, tyrosine hydroxylase- and parvalbumin-immunoreactive neurons were not markedly changed. Our results indicate that: (1) the AT overexpression after the chronic constriction injury is an important factor in Angiotensin II-potentiated pain perception; (2) Angiotensin II is involved in pathological mechanisms of neuropathic pain and this effect can be mediated perhaps in combination with other neuropeptides synthesized in the primary sensory neurons.

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Section: Cell Countingmentioning

confidence: 99%

The Characterization of AT1 Expression in the Dorsal Root Ganglia After Chronic Constriction Injury

et al. 2016

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“…First, according to gate control theory, application of vibration may reduce orthodontic pain by stimulating inhibitory interneurons, which would reduce the amount of pain signals transmitted by Aδ and c fibers. Second, exposure to repeated vibration triggers axonal retrograde-transport in primary sensory neurons and subsequently decreases the release of nociceptive neuropeptides, and thereby reduces nociceptive transmission (14). Since some neuropeptides are potent peptide vasodilators, vibration-induced reductions in the levels of nociceptive neuropeptides may also decrease the area of periodontal ligament compressed by temporary displacement of the teeth-including nerve fibers and occluded blood vessels-and thus promote blood flow (25).…”

Section: Discussionmentioning

confidence: 99%

“…Based on the theory of gate control, an appropriate vibration can activate mechanoreceptors, such as Meissner's corpuscle in subcutaneous and bone tissue, which will stimulate largediameter Aβ nerve fibers to initiate inhibitory interneurons that, in turn, reduce pain perception by inhibiting nociceptive signals transmitted from projection neurons to the brain (13). In addition, there is evidence that vibration may act as analgesic by triggering retrograde transport within primary sensory neurons and subsequently reduce the release of nociceptive neuropeptides (14). However, the ability of vibration to relieve orthodontic pain has only been assessed in a limited number of studies.…”

Section: Vanilloid 1 (Trpv1) Ion Receptor Is Involved In the Release mentioning

confidence: 99%

Interval Vibration Reduces Orthodontic Pain Via a Mechanism Involving Down-regulation of TRPV1 and CGRP

Thammanichanon

Kaewpitak

Binlateh

et al. 2020

In Vivo

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“…PV is often used as a marker of a subpopulation of (muscle) proprioceptive afferents, comprising 10–33 % of cells in spinal and trigeminal ganglia [ 11 , 19 , 22 , 23 , 33 , 37 , 38 ]. This study found that 25 % of naïve rat L4-5 DRGs were PV-ir, consistent with these earlier reports.…”

Section: Discussionmentioning

confidence: 99%

Effects of peripheral nerve injury on parvalbumin expression in adult rat dorsal root ganglion neurons

Medici

Shortland

2015

BMC Neurosci

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BackgroundParvalbumin (PV) is a calcium binding protein that identifies a subpopulation of proprioceptive dorsal root ganglion (DRG) neurons. Calcitonin gene-related peptide (CGRP) is also expressed in a high proportion of muscle afferents but its relationship to PV is unclear. Little is known of the phenotypic responses of muscle afferents to nerve injury. Sciatic nerve axotomy or L5 spinal nerve ligation and section (SNL) lesions were used to explore these issues in adult rats using immunocytochemistry.ResultsIn naive animals, the mean PV expression was 25 % of L4 or L5 dorsal root ganglion (DRG) neurons, and this was unchanged 2 weeks after sciatic nerve axotomy. Colocalization studies with the injury marker activating transcription factor 3 (ATF3) showed that approximately 24 % of PV neurons expressed ATF3 after sciatic nerve axotomy suggesting that PV may show a phenotypic switch from injured to uninjured neurons. This possibility was further assessed using the spinal nerve ligation (SNL) injury model where injured and uninjured neurons are located in different DRGs. Two weeks after L5 SNL there was no change in total PV staining and essentially all L5 PV neurons expressed ATF3. Additionally, there was no increase in PV-ir in the adjacent uninjured L4 DRG cells. Co-labelling of DRG neurons revealed that less than 2 % of PV neurons normally expressed CGRP and no colocalization was seen after injury.ConclusionThese experiments clearly show that axotomy does not produce down regulation of PV protein in the DRG. Moreover, this lack of change is not due to a phenotypic switch in PV immunoreactive (ir) neurons, or de novo expression of PV-ir in uninjured neurons after nerve injury. These results further illustrate differences that occur when muscle afferents are injured as compared to cutaneous afferents.

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The impact of short-lasting repeated vibrations on retrograde axonal transport, the expression of CGRP and parvalbumin in lower lumbar dorsal root ganglia

Cited by 6 publications

References 20 publications

The Characterization of AT1 Expression in the Dorsal Root Ganglia After Chronic Constriction Injury

The Characterization of AT1 Expression in the Dorsal Root Ganglia After Chronic Constriction Injury

Interval Vibration Reduces Orthodontic Pain Via a Mechanism Involving Down-regulation of TRPV1 and CGRP

Effects of peripheral nerve injury on parvalbumin expression in adult rat dorsal root ganglion neurons

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