The impact of short-term refrigeration of human lipoaspirate on adipose-derived stem cells and adipocytes

Wang, Wei Z.; Fang, Xiaojie; Williams, Shelley; Stephenson, Linda L.; Baynosa, Richard C.; Jaeger, Nolan; Khiabani, Kayvan T.; Zamboni, William A.

doi:10.1016/j.bjps.2014.08.070

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…This observation is confirmed by others (Ref. 51). Thus, where the adipose tissue needs to be transported for processing at distant locations, we recommend shipping at room temperature.…”

Section: Isolation Of Stem Cellssupporting

confidence: 89%

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Porsborg

Zachar

Boucher

et al. 2015

Expert Rev. Mol. Med.

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Since the discovery of adipose-derived stem cells (ASCs), there have been high expectations of their putative clinical use. Recent advances support these expectations, and it is expected that the transition from pre-clinical and clinical studies to implementation as a standard treatment modality is imminent. However ASCs must be isolated and expanded according to good manufacturing practice guidelines and a basic assurance of quality, safety, and medical effectiveness is needed for authorisation by regulatory agencies, such as European Medicines Agency and US Food and Drug Administration. In this review, a collection of studies investigating the influence of different steps of the isolation and expansion protocol on the yield and functionality of ASCs has been presented in an attempt to come up with best recommendations that ensure potential beneficial clinical outcome of using ASCs in any therapeutic setting. If the findings confirm the initial observations of beneficial effects of ASCs, the path is paved for implementing these ASC-based therapies as standard treatment options.

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“…This observation is confirmed by others (Ref. 51). Thus, where the adipose tissue needs to be transported for processing at distant locations, we recommend shipping at room temperature.…”

Section: Isolation Of Stem Cellssupporting

confidence: 89%

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Porsborg

Zachar

Boucher

et al. 2015

Expert Rev. Mol. Med.

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“…Nevertheless, they showed some interesting data that could be pertinent for our study. The studies of Wang and Matsumoto reported short-term viability or morphology of adipocytes, reporting similar results with refrigerated vs non-refrigerated samples 13,15 .…”

Section: Discussionmentioning

confidence: 63%

“…Other studies reported viability of adipocytes within few hours after cryopreservation, but using liquid nitrogen at -140 °C 14 Wang and Cols. studied the viability of adipocytes at 2-8 °C for up to 96 h (24-96 h), reporting similar viability in refrigerated versus non refrigerated samples, although they found decreased metabolic activity overtime 15 . Matsumoto and Cols preserved lipoaspirates at room temperature for up to 24 h and at 4 °C for 24 to 72 h, to assess morphological changes and metabolic activity 13 .…”

Section: Introductionmentioning

confidence: 99%

Efecto de la refrigeración en la viabilidad y la apoptosis de los adipocitos decantados después de la liposucción

Mecott¹,

Chacón-Martínez²,

Leal-Torres³

et al. 2019

CIRU

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Objective: To evaluate the effect of refrigeration in the apoptosis and viability of the lipoaspirate in the first 2 h after harvesting. Methods: 20 consecutive patients who underwent liposuction from the abdomen for esthetic reasons were included. 5 ml of fat were obtained and processed for study. The viability was obtained using trypan blue. Apoptosis was determined using TUNEL assay. Results: All patients were female with a median age of 36.5 (21-67) years. On regard of the viability, at time 0, the viability in the control group was 59.08 ± 24% and 60.96 ± 22% in the refrigeration group. At 60 min, the values were 50.82 ± 21% versus 55 ± 32.6% (p = 0.74) and a 120 min, 42.69 ± 20.85% and 50.33 ± 21% respectively. On regard of apoptosis, the percentage of apoptotic cells at time 0 was 37.87 ± 9.7% for the control group and 34.28 ± 9.74% for refrigerated samples. At 60 min 51.11 ± 8.64% versus 45.94 ± 9.15% and at 120 min, 62.97 ± 13.33% versus 55.81 ± 9.45% respectively. Conclusions: Refrigerating the lipoaspirate at 4 °C decreased the mortality and apoptosis of the adipocytes in <10% within the first 2 h from harvesting.

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“…Despite this limitation, our results are consistent with recent prospective randomized clinical trials reporting the lack of benefit of surgical drain placement in patients undergoing gastrectomy for cancer. (33,34) Furthermore, an expanded retrospective study showed that routine drains likely have no benefit after LRYGB. (19) In a recent systematic review, Messager and colleagues also reported that there was no evidence to favor intraabdominal drainage after gastrectomy with respect to morbidity and mortality, nor was it helpful in the detection or management of leaks.…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Gastrointestinal Leak after Bariatric Surgery: MBASQIP Analysis

Alizadeh

Inaba

et al. 2018

Journal of the American College of Surgeons

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The impact of short-term refrigeration of human lipoaspirate on adipose-derived stem cells and adipocytes

Cited by 6 publications

References 5 publications

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Efecto de la refrigeración en la viabilidad y la apoptosis de los adipocitos decantados después de la liposucción

Risk Factors for Gastrointestinal Leak after Bariatric Surgery: MBASQIP Analysis

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