The purpose of this study is to reveal the relationship between the perception of organizational support, job performance and work-family conflict for female employees. For this purpose, data were collected via a survey technique from 176 female employees working at Bayburt University. The obtained data were analyzed using SPSS and AMOS statistical package programs. The findings of the study which used the structural equation model indicate that the perception of organizational support negatively and significantly affects work-family conflict and positively and significantly affects work performance; It has been revealed that work-family conflict negatively and significantly affects work performance. In addition, the study also concluded that work-family conflict plays a full mediating role in the relationship between the perception of organizational support and job performance. In other words, the perception of organizational support was found to lead to an increase in job performance by reducing work-family conflict (mediating variable).