“…Therefore, the present work represents the first approach to spontaneous fermentation Saccharomyces population dynamics in Tikveš region, the most important vinicultural region in Macedonia that has never been characterized before. Our previous report (Ilieva, Kostadinović Veličkovska, Dimovska, & Spasov, 2016) indicated third-stage selected yeast strain F-78 as the best strain for alcoholic fermentation of Vranec wines. In the present study, the isolation of 80 yeast strains from spontaneously fermented grape must from 10 different micro-regions of Tikveš vinicultural region was applied and the fermentation activity of the yeast was studied.…”