The impact of the omics era on the knowledge and use of<i>Lysobacter</i>species to control phytopathogenic micro-organisms

Puopolo, Gerardo; Tomada, Selena; Pertot, I.

doi:10.1111/jam.13607

Cited by 70 publications

(42 citation statements)

References 83 publications

(190 reference statements)

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“…The genera exclusive for the agricultural soil were Lysobacter and Aeromicrobium . The genus Lysobacter is commonly found in agricultural soils [52], and their abundance is strongly modulated by soil type and negatively affected by low pH [53, 54]. Liming is a common agricultural practice in tropical croplands to increase soil pH [55] and is also typically applied in the region in Colombia where the agricultural soil used in our study was collected.…”

Section: Discussionmentioning

confidence: 99%

Deciphering rhizosphere microbiome assembly of wild and modern common bean (Phaseolus vulgaris) in native and agricultural soils from Colombia

et al. 2019

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Background Modern crop varieties are typically cultivated in agriculturally well-managed soils far from the centers of origin of their wild relatives. How this habitat expansion impacted plant microbiome assembly is not well understood. Results Here, we investigated if the transition from a native to an agricultural soil affected rhizobacterial community assembly of wild and modern common bean ( Phaseolus vulgaris ) and if this led to a depletion of rhizobacterial diversity. The impact of the bean genotype on rhizobacterial assembly was more prominent in the agricultural soil than in the native soil. Although only 113 operational taxonomic units (OTUs) out of a total of 15,925 were shared by all eight bean accessions grown in native and agricultural soils, this core microbiome represented a large fraction (25.9%) of all sequence reads. More OTUs were exclusively found in the rhizosphere of common bean in the agricultural soil as compared to the native soil and in the rhizosphere of modern bean accessions as compared to wild accessions. Co-occurrence analyses further showed a reduction in complexity of the interactions in the bean rhizosphere microbiome in the agricultural soil as compared to the native soil. Conclusions Collectively, these results suggest that habitat expansion of common bean from its native soil environment to an agricultural context had an unexpected overall positive effect on rhizobacterial diversity and led to a stronger bean genotype-dependent effect on rhizosphere microbiome assembly. Electronic supplementary material The online version of this article (10.1186/s40168-019-0727-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Deciphering rhizosphere microbiome assembly of wild and modern common bean (Phaseolus vulgaris) in native and agricultural soils from Colombia

et al. 2019

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“…The genus Lysobacter ( Christensen and Cook, 1978 ) consists of bacteria isolated from various environments like freshwater, agricultural soils and plant rhizosphere ( Reichenbach, 2006 ; Puopolo et al, 2018 ). Notably, the occurrence of bacterial strains belonging to the species L. antibioticus , L. capsici , and L. gummosus in clay agricultural soils, was related to the disease suppressiveness against R. solani ( Postma et al, 2008 , 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Volatile Organic Compounds From Lysobacter capsici AZ78 as Potential Candidates for Biological Control of Soilborne Plant Pathogens

Vlassi

Nesler²,

Perazzolli

et al. 2020

Front. Microbiol.

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The genus Lysobacter includes several bacterial species which show potential for being used in biological control of plant diseases. It was shown recently that several Lysobacter type strains produce volatile organic compounds (VOCs) which controlled the growth of Phytophthora infestans in vitro when the bacteria were grown on a protein rich medium. In the present study, Lysobacter capsici AZ78 (AZ78) has been tested for its potential to produce VOCs that may contribute to the bioactivity against soilborne plant pathogens. To this end, split Petri dish assays of bacterial cultures have been combined with GC-MS measurements with the aim to reveal the identity of the VOCs which inhibit the growth of Pythium ultimum Rhizoctonia solani, and Sclerotinia minor. While AZ78 completely suppressed the growth of P. ultimum and S. minor, the growth of R. solani was still reduced significantly. The GC-MS analysis revealed 22 VOCs to be produced by AZ78, the majority of which were (putatively) identified as mono-and dialkylated methoxypyrazines. Based on additional cultivation and GC-MS experiments, 2,5-dimethylpyrazine, 2-ethyl-3-methoxypyrazine and 2-isopropyl-3-methoxypyrazine were selected as presumable bioactive compounds. Further bioassays employing indirect exposure to standard solutions (1-10 mg per Petri dish) of the synthetic compounds via the gas phase, revealed that each of these pyrazines was able to suppress the growth of the pathogens under investigation. The results of this study highlight the possible future implementation of pyrazine derivatives in the control of soilborne plant diseases and further support the biocontrol potential of L. capsici AZ78.

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“…Production of lytic enzymes and antibiotics is also typical of the predation implemented by BCAs belonging to the genus Lysobacter (Puopolo et al 2018). Thus, L. capsici AZ78 is able to colonize Ph.…”

Section: Current State Of Biocontrol Strategiesmentioning

confidence: 99%

Bioformulation of Microbial Biocontrol Agents for a Sustainable Agriculture

Bejarano

Puopolo

2020

Progress in Biological Control

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The application of microbial based biopesticides has become a sustainable alternative to the use of chemicals to prevent yield losses due to plant pathogens. However, microbial based biopesticides are often unsuccessfully formulated and do not meet the demanding regulatory standards required by the agencies, which hinders their commercialization. Hence, an outline on the approaches to attain more effective formulations might be useful for the development of future more effective products.With this aim, this chapter reports the current state of biocontrol strategies and describes the principles of microbial biocontrol formulations. Emphasis is placed on techniques and tools available for the development and characterisation of microbial products. To provide glimpses on the possible formulations, the different existing additives, carriers, inoculation techniques and formulation types are exhaustively reviewed. Finally, requirements and principles for efficacy evaluation of plant protection products in the European Union are included (see Chaps. 10.1007/978-3-030-53238-3_11, 10.1007/978-3-030-53238-3_18 and 10.1007/978-3-030-53238-3_19).

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The impact of the omics era on the knowledge and use ofLysobacterspecies to control phytopathogenic micro-organisms

Cited by 70 publications

References 83 publications

Deciphering rhizosphere microbiome assembly of wild and modern common bean (Phaseolus vulgaris) in native and agricultural soils from Colombia

Deciphering rhizosphere microbiome assembly of wild and modern common bean (Phaseolus vulgaris) in native and agricultural soils from Colombia

Volatile Organic Compounds From Lysobacter capsici AZ78 as Potential Candidates for Biological Control of Soilborne Plant Pathogens

Bioformulation of Microbial Biocontrol Agents for a Sustainable Agriculture

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