The impact of three bacteria isolated from contaminated plant cultures on in vitro multiplication and rooting of microshoots of four ornamental plants

Zawadzka, Marta; Trzciński, Paweł; Nowak, Katarzyna; Orlikowska, T.

doi:10.2478/johr-2013-0020

Cited by 17 publications

(9 citation statements)

References 59 publications

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“…Moreover, it is able to detoxify and remove chlorpyrifos residues in infested rice plants and grains (Feng et al, 2017). In tissue cultures, C. pusillum stimulated axillary shoot formation in chrysanthemum, gerbera, and hosta (Zawadzka et al, 2013). Cucumber seed treatment with a co-inoculum of Curtobacterium with other PGPRs (C. flaccumfaciens strain ME1 x B. pumilus strains INR7 x B. subtilis strain GB03) resulted in intensive plant growth promotion and reduction of three cucumber pathogens, Colletotrichum orbiculare (causing anthracnose), Pseudomonas syringae pv.…”

Section: Introductionmentioning

confidence: 99%

Biological control of septoria leaf blotch and growth promotion in wheat by Paenibacillus sp. strain B2 and Curtobacterium plantarum strain EDS

Samain¹,

Tuinen

Jeandet

et al. 2017

Biological Control

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Keywords:Wheat septoria leaf blotch Paenimyxin Paenibacillus sp. strain B2 Curtobacterium plantarum Flavonoids Salicylic acid Jasmonic acid Reactive oxygen species A B S T R A C T Many of the non-pathogenic endophytic bacteria that reside in plant roots promote plant growth as well as protection against pathogens attack. However, little is known about their mode of action in wheat. We have previously demonstrated the potential of Paenibacillus sp. strain B2 (PB2) to stimulate plant defense mechanisms via its paenimyxin lipo-polypeptide elicitor. Recently, we isolated the Curtobacterium plantarum strain EDS (EDS) from seeds of almost all wheat cultivars. In the present work, the ability of PB2 and EDS to promote wheat growth and protection against Septoria leaf blotch (SLB) was investigated. Results showed that PB2 is a general root external colonizer and cultivar-dependent endophyte. In the endophytic state and only in co-inoculation, it significantly increased the internal root colonization by EDS, resulting in an increase of root and aerial part fresh weights. qPCR analysis showed that, in the endophytic and nonendophytic states, PB2 conferred ≥59% protection against SLB by inducing systemic resistance which is characterized by the over expression of the pr1, lox, Aos, peroxidase, oxo and gst genes. Paenimyxin conferred 76% local protection characterized by the overexpression of the glu, lox, aos, pal, chs, oxo, and gst genes, and 82% systemic protection by chs. It was concluded that PB2 is potentially very interesting in the biocontrol of SLB and, in a mixture with EDS, in the wheat growth promoting. Genes involved in the flavonoid, salicylic acid, jasmonic acid, reactive oxygen species and basal defense pathways seem to play an important role in the resistance against SLB.

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Section: Introductionmentioning

confidence: 99%

Biological control of septoria leaf blotch and growth promotion in wheat by Paenibacillus sp. strain B2 and Curtobacterium plantarum strain EDS

Samain¹,

Tuinen

Jeandet

et al. 2017

Biological Control

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“…None of the isolates had a negative effect on the growth of host plants. In another study [55], in the optimization of a scheme for in vitro production propagation of wild indigo Baptisia tinctoria medicinal plant, it was shown that the application of arbuscular mycorrhizal fungi increased the acclimatization percentage of microshoots and rooted microplants.…”

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confidence: 99%

ENDOPHYTES, AS PROMOTORS OF in vitro PLANT GROWTH (review)

Самарина¹,

Malyarovskaya²,

Rogozhina³

et al. 2017

S-h. biol.

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In vitro plant propagation is a developed biotechnology, however until now there are no effective protocols for many perennials, especially for trees. High contamination of mature explants during tissue culture initiation, low multiplication and rooting during following passages are the main challenges. Aseptic culture of explants is associated with stress due to tissue damage and exposition to aggressive disinfectants and antibiotics during initiation. These could be the reasons of virulence of endophytes in following propagation. Plant-associated microorganisms were until recently seen as a problem for micropropagation, leading to contamination of in vitro explants. However recent studies showed that colonization of endophytes often play crucial role for increasing viability of in vitro and ex vitro plants. Most endophytes affect positively plant growth, providing nutrients and exhibiting antagonism to pathogens, as well as decreasing stress effects on plants. Beneficial effects were obtained in using Beauveria bassiana (

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“…Дуже сухі або засолені ґрунти унеможливлюють їх ефективне використання як орних земель через пригнічення росту рослин. На сьогодні вже було ефективно доведено, що деякі бактерії можна успішно застосовувати для пом'якшення подібних стресових умов [78,80,86].…”

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“…Ці та інші бактерії були виділені та випробувані на культурах троянди, хризантеми та гербери in vitro. Жодна з бактерій не завдала шкоди експлантам, і деякі комбінації бактерій та видів рослин суттєво покращили розмноження та вкорінення [86]. У цьому дослідженні бактерії роду Curtobacterium збільшили проліферацію пагонів рослин усіх трьох видів, а також вкорінення троянди, Methylobacterium підвищили продуктивність гербери, а Bacillus підвищили довжину пагонів хризантем.…”

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