The impact of ultrasound pretreatment on the enzymatic hydrolysis of cellulose from sugar beet shreds: Modeling of the experimental results

Abstract

This article investigates the impact of ultrasound as pretreatment for enzymatic hydrolysis of cellulose from sugar beet shreds and uses modeling of the experimental results. Ultrasound pretreatment with different power input, duration, duty cycle, and solids load was applied to sugar beet shreds. Ultrasound under investigated conditions provided substrates with different characteristics. At applied conditions, ultrasound caused up to 28% material solubilization while cellulose recovery was approximately 90%. Pretreated substrates had multiple times higher values of water retention (WRV) than untreated one. Ultrasound pretreated sugar beet shreds were more susceptible to cellulases adsorption than the untreated ones having 1.4 to 15 times higher maximum adsorption capacity (σ). Statistical modeling of the results of enzymatic hydrolysis revealed that parameters with significant influence on the reducing sugars yield were interaction between σ and cellulose recovery, then σ and WRV as mutually independent parameters, as well as interaction between WRV and σ. The highest achieved yield of cellulose hydrolysis was 3.7 times higher than that obtained with untreated sugar beet shreds and it amounted approximately 780 mg/g cellulose. © 2017 American Institute of Chemical Engineers Environ Prog, 36: 1164–1172, 2017

Recent Trends in the Pretreatment of Lignocellulosic Biomass for Value-Added Products

“…They found the combination of 170 kHz for 0.5 h and 40 kHz for 1.5 h and a power of 1,000 W as adequate, resulting 12% increased yield of bio-oil as compared to untreated wood. However, ultrasonication for a prolonged period might cause adverse effect due to collision and aggregation between the particles (Ivetić et al, 2017). Li,kewise, high sonication power leads to cavitation near the ultrasound transducer tip that prohibits the energy transfer to the liquid medium (Subhedar and Gogate, 2014).…”

“…Li,kewise, high sonication power leads to cavitation near the ultrasound transducer tip that prohibits the energy transfer to the liquid medium (Subhedar and Gogate, 2014). Another study on the ultrasound pretreatment on sugar beet shreds followed by enzymatic hydrolysis resulted in a yield of 780 mg/g cellulose, which was 3.7 times higher than that of achieved with untreated samples (Ivetić et al, 2017). Luzzi et al (2017) represented an interesting work on pretreatment of LCB where they performed the ultrasonication treatment and enzymatic hydrolysis in a single step.…”

The Role of Ionic Liquids in the Lignin Separation from Lignocellulosic Biomass

“…However, collision and aggregation could be caused between the particles as a result of prolonged use of ultrasonication. [63]. The hydrolysis time of biomass can be decreased up to 80% by the use of ultrasound, which in return adds benefit to bio-fuel production [41].…”

Solutions for improvement of saccharification and fermentation of high gravity rye mashes

“…Ultrasonic pretreatment also induces changes in the structure of the lignin-hemicellulose complex (Ebringerová and Hromádková, 2002;Ivetić et al, 2017). Extraction of polysaccharides, including cellulose and arabinoxylans, caused by the phenomenon of cavitation, can increase the efficiency of their further hydrolysis with the use of enzyme preparations.…”

“…Moreover, during ultrasonic pretreatment their partially hydrolysis can occur. Ultrasound application to sugar beet shreds treatment in research carried by Ivetić et al (2017) affected the loss in substrate weight which was caused by partial degradation and solubilisation of some components. Results showed, among others, lower (by 25%) xylan content in the obtained medium.…”