2024
DOI: 10.21608/djvs.2024.266721.1129
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The Impact of Using Extenders Containing Low-density Lipoprotein Versus Egg Yolk with or without Ascorbic Acid on the Fertility Parameters of Post-thawed Chilled Canine Semen

Mohamed Elbehiry,
Ayman Abd El-Aziz,
Mohamed Belal
et al.

Abstract: Egg yolk is included in semen extenders because it prevents cold shock during chilling. This study aimed to assess the impact of substituting egg yolk with low-density lipoprotein (LDL) in Tris-Citric-Fructose (TCF) chilled extenders, with or without ascorbic acid, on the canine semen quality at 24, 48, and 72 hours of cooling. Semen from three German shepherd dogs was evaluated and extended at a 1:1 ratio using four extenders: Group A (TCF buffer, 20% egg yolk, with ascorbic acid), Group B (TCF buffer, 20% eg… Show more

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