The impact of zearalenone and thidiazuron on indirect plant regeneration of oilseed rape and wheat

Abstract

The aim of the study was to test the synergistic activity of zearalenone and thidiazuron with commonly used regulators (6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid) in callus tissue growth and the regeneration processes of winter oilseed rape and winter wheat. Oilseed rape callus was obtained from the upper fragments of seedling hypocotyls. Wheat callus tissue was obtained from immature embryos. Zearalenone, in combination with 6-benzylaminopurine, stimulated the regeneration of oilseed rape and, in combination with 2,4-dichlorophenoxyacetic acid, the regeneration of wheat. An additional application of thidiazuron to these media increased the percentage of shoot regeneration from callus in both species tested. Zearalenone is proposed as a new regulator in in vitro plant cultures for use in protocols of plant regeneration.

Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

“…TDZ in combination with auxin (2,4-D) (RM4) induced the highest regeneration frequency (97.3 %) with an average of 10 shoots per mature embryo. Similar beneficial effect of TDZ in combination of 2,4-D was observed on shoot regeneration from wheat immature embryo-derived callus by Biesaga-Kościelniak et al (2010). Our results also corroborated the earlier work that TDZ should be used in combination with other growth regulators during the regeneration phase in mature embryo culture (Chauhan et al 2007).…”

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

“…Previously, we found that the process of green plant regeneration was moderated by the presence of Cu 2+ and Ag + ions in the medium, and depended on the time duration of the in vitro anther culture [58] (under review). Acquisition of plants via in vitro cultures can occur from explants by direct organogenesis or embryogenesis without previously formed callus [59,60] or vicariously, when new shoots or embryos occurred on already formed callus (indirect organogenesis or embryogenesis) [61]. It is being suggested that under tissue culture conditions direct embryogenesis may proceed an indirect one.…”

High production of bioactive depsides in shoot and callus cultures of Aronia arbutifolia and Aronia × prunifolia

“…This phenomenon nowadays is often studied as an important factor in green biotechnology. PGRs play a key role in the research focused on plant micropropagation (Gaj 2004;Sujatha and Kumari 2007;Biesaga-Kościelniak et al 2010;Jana and Shekhawat 2011). The influence of the concentrations of GRs on the accumulation of different biogenetic groups of plant metabolites is an eminent fact (Ramawat and Mathur 2007).…”