The impacts of ozonation on oil sands process-affected water biodegradability and biofilm formation characteristics in bioreactors

Hwang, Geelsu; Dong, Tao; Islam, Md. Shahinoor; Sheng, Zhiya; Pérez-Estrada, Leónidas A.; Liu, Yang; El-Din, Mohamed Gamal

doi:10.1016/j.biortech.2012.12.005

Cited by 104 publications

(82 citation statements)

References 30 publications

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“…The biofilm images indicate that the bacteria did not fully cover (i.e., completely green and red images) the GAC surface even after 48 days. The biofilm thicknesses are similar to those found by Hwang et al (22) for OSPW biofilms produced on polyethylene (PE) and polyvinylchloride (PVC) coupons in continuous bioreactors, where a continuous (full covered) biofilm on the surface was observed on these relatively smooth surfaces. This is expected, as the bacteria form a complex structure of aerobic biofilms formed by discrete aggregates of densely packed cells and interstitial voids on the GAC surfaces as reported previously (38,39).…”

Section: Methodssupporting

confidence: 86%

“…The images were observed and scanned randomly at 4 or 5 positions with a Zeiss Plan-Apochromat lens (ϫ20 magnification, 0.8 numerical aperture). The detailed procedure has been described previously by Hwang et al (22).…”

Section: Methodsmentioning

confidence: 99%

“…For example, a very limited number of sequences can be generated by the DGGE and clone library methods, and the processes are time-consuming (20,21). Preferential amplification of rRNA genes with the PCR-based methods may lead to the omission of some microbial species information (21,22). T-RFLP analysis is PCR based and suffers from the same drawbacks as this technique (23).…”

mentioning

confidence: 99%

“…However, few studies have addressed biofilm community analysis for bioreactors aimed at treating OSPW. Among these studies, the DGGE technique has been utilized for the analysis of OSPW biofilm microbial communities on various surfaces, such as polyethylene (PE) (22,27,28), polyvinyl chloride (PVC) (22,28), and GAC (15,16). However, to our knowledge, no studies have investigated OSPW biofilm formation on GAC using high-throughput pyrosequencing techniques.…”

mentioning

confidence: 99%

“…In addition, it is well known that ozone can be used to degrade the target classical NAs with high cyclization and long chains into lower-molecularweight oxidized NA compounds, thus increasing their biodegradability (16,29). Moreover, ozonation can impact the microbial community structure in biofilm reactors and has the potential to increase bioreactor performance (22). Thus, the impact of ozonated OSPW on biofilm growth, biofilm community structure, and overall NA removal was also evaluated to assess the synergistic removal by these individual OSPW treatment processes.…”

mentioning

confidence: 99%

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Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Islam

Zhang

McPhedran

et al. 2015

Appl Environ Microbiol

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The development of biodegradation treatment processes for oil sands process-affected water (OSPW) has been progressing in recent years with the promising potential of biofilm reactors. Previously, the granular activated carbon (GAC) biofilm process was successfully employed for treatment of a large variety of recalcitrant organic compounds in domestic and industrial wastewaters. In this study, GAC biofilm microbial development and degradation efficiency were investigated for OSPW treatment by monitoring the biofilm growth on the GAC surface in raw and ozonated OSPW in batch bioreactors. The GAC biofilm community was characterized using a next-generation 16S rRNA gene pyrosequencing technique that revealed that the phylum Proteobacteria was dominant in both OSPW and biofilms, with further in-depth analysis showing higher abundances of Alpha-and Gammaproteobacteria sequences. Interestingly, many known polyaromatic hydrocarbon degraders, namely, Burkholderiales, Pseudomonadales, Bdellovibrionales, and Sphingomonadales, were observed in the GAC biofilm. Ozonation decreased the microbial diversity in planktonic OSPW but increased the microbial diversity in the GAC biofilms. Quantitative real-time PCR revealed similar bacterial gene copy numbers (>10 9 gene copies/g of GAC) for both raw and ozonated OSPW GAC biofilms. The observed rates of removal of naphthenic acids (NAs) over the 2-day experiments for the GAC biofilm treatments of raw and ozonated OSPW were 31% and 66%, respectively. Overall, a relatively low ozone dose (30 mg of O 3 /liter utilized) combined with GAC biofilm treatment significantly increased NA removal rates. The treatment of OSPW in bioreactors using GAC biofilms is a promising technology for the reduction of recalcitrant OSPW organic compounds.O pen-pit mining of Canada's Athabasca oil sands produces large quantities of bitumen coated sands. The bitumen is extracted from the sands using the Clark hot-water process, in which 1 m 3 of bitumen extracted from oil sands generates 4 m 3 of oil sands process-affected water (OSPW) (1-3). Over a billion cubic meters of this generated OSPW is currently stored in tailing ponds given the no-release policy for OSPW followed by the oil sands companies (4, 5). This volume of OSPW will continually increase as the oil sands bitumen extraction continues in the Athabasca region until suitable treatment technologies become available to allow for OSPW release (2). This OSPW is contaminated with a large number of inorganic and organic compounds during the extraction process that are known to be toxic to aquatic and terrestrial life (6). Thus, the production of OSPW associated with the extraction of oil sands bitumen is a great environmental issue that needs to be addressed in the near future for the remediation of the oil sands region.Fresh OSPW has acute, subchronic, and chronic toxicities to aquatic organisms, and the majority of this toxicity has been previously attributed to naphthenic acids (NAs) (2, 7). NAs are a mixture of organic surfactants containing carboxy...

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Section: Methodssupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Islam

Zhang

McPhedran

et al. 2015

Appl Environ Microbiol

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A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

Dhall

Ramjee

et al. 2022

Adv Materials Technologies

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Biofilms are communities of microbes that colonize surfaces. While several biofilm experimental models exist, they often have limited replications of spatiotemporal dynamics surrounding biofilms. For a better understanding dynamic and complex biofilm development, this manuscript presents a customizable platform compatible with off‐the‐shelf well plates that can monitor microbial adhesion, growth, and associated parameters under various relevant scenarios by taking advantage of 3D printing. The system i) holds any substrate in a stable, vertical position, ii) subjects samples to flow at different angles, iii) switches between static and dynamic modes during an experiment, and iv) allows multiplexing and real‐time monitoring of biofilm parameters. Simulated fluid dynamics is employed to estimate flow patterns around discs and shear stresses at disc surfaces. A 3D printed peristaltic pump and a customized pH measurement system for real‐time tracking of spent biofilm culture media are equipped with a graphical user interface that grants control over all experimental parameters. The system is tested under static and dynamic conditions with Streptococcus mutans using different carbon sources. By monitoring the effluent pH and characterizing biochemical, microbiological, and morphological properties of cultured biofilms, distinct properties are demonstrated. This novel platform liberates designing experimental strategies for investigations of biofilms under various conditions.

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Application of mass balance models in the process of ozone removal of naphthenic acids from water

jibouri

Upreti

2016

Can J Chem Eng

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The oil sands process‐affected water (OSPW), produced in large amounts during the extraction of crude oil from oil sands, is toxic mainly due to the presence of naphthenic acids (NAs), among other constituents. Ozonation is an effective method for the removal of NAs. To provide information for better control of NAs ozonation, models based on mass balance were developed in this study to predict the concentration profiles of commercial NAs, dissolved ozone, and gaseous ozone during the process. It was found that the developed models successfully predicted the concentrations of commercial NAs and gaseous ozone. For the dissolved ozone concentration, the developed model can predict the equilibrium concentrations well. However, the actual ozone consumption was higher than that predicted by the model at the initial period of the ozonation process. This deviation possibly resulted from the inapplicability of the gas‐liquid equilibrium conditions at the initial high rate stage of the reaction. In addition, the Henry's law constant and the overall mass transfer coefficient for the given system were determined experimentally. Also investigated in this study was the effects of inlet gaseous ozone concentration into the reactor on the removal of NAs and on the concentration of dissolved ozone. As expected, the increase in inlet ozone concentration enhanced the removal of NAs and increased the concentration of dissolved ozone.

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The impacts of ozonation on oil sands process-affected water biodegradability and biofilm formation characteristics in bioreactors

Cited by 104 publications

References 30 publications

Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

Application of mass balance models in the process of ozone removal of naphthenic acids from water

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