The implantation of cartilaginous and periosteal tissue into growth plate defects

Wirth, Thomas; Byers, Sharon; Byard, Roger W.; Hopwood, John J.; Foster, Bruce K.

doi:10.1007/bf00188326

Cited by 37 publications

(13 citation statements)

References 33 publications

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“…Previous ovine studies have determined an infiltration of inflammatory cells 4 days post surgery, and ossification of the defect site within 14 days [11-13], comparable to that observed in rats [19]. To date, successful repair of growth plate defects using MSC has been achieved using agarose, chitin, gelatin and Gelfoam composites, and fibrin glue [3-6, 28].…”

Section: Discussionmentioning

confidence: 78%

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Application of Autologous Bone Marrow Derived Mesenchymal Stem Cells to an Ovine Model of Growth Plate Cartilage Injury

McCarty¹,

Chen²,

Gronthos³

et al. 2010

TOORTHJ

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Injury to growth plate cartilage in children can lead to bone bridge formation and result in bone growth deformities, a significant clinical problem currently lacking biological treatment. Mesenchymal stem/stromal cells (MSC) offer a promising therapeutic option for regeneration of damaged cartilage, due to their self renewing and multi-lineage differentiation attributes. Although some small animal model studies highlight the therapeutic potential of MSC for growth plate repair, translational research in large animal models, which more closely resemble the human condition, are lacking. Our laboratory has recently characterised MSCs derived from ovine bone marrow, and demonstrated these cells form cartilage-like tissue when transplanted within the gelatin sponge, Gelfoam, in vivo. In the current study, autologous bone marrow MSC were seeded into Gelfoam scaffold containing TGF-β1, and transplanted into a surgically created defect of the proximal ovine tibial growth plate. Examination of implants at 5 week post-operatively revealed transplanted autologous MSC failed to form new cartilage structure at the defect site, but contributed to an increase in formation of a dense fibrous tissue. Importantly, the extent of osteogenesis was diminished, and bone bridge formation was not accelerated due to transplantation of MSCs or the gelatin scaffold. The current study represents the first work that has utilised this ovine large animal model to investigate whether autologous bone marrow derived MSC can be used to initiate regeneration at the injured growth plate.

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Section: Discussionmentioning

confidence: 78%

“…Several interpositional materials have been assessed for their potential to impede or prevent bone formation and regenerate the damaged cartilage. These include fat, cultured chondrocytes [7, 9], cartilage [11], periosteum [11], and a type 1 collagen paste [12]. …”

Section: Introductionmentioning

confidence: 99%

Application of Autologous Bone Marrow Derived Mesenchymal Stem Cells to an Ovine Model of Growth Plate Cartilage Injury

McCarty¹,

Chen²,

Gronthos³

et al. 2010

TOORTHJ

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“…The first studies dealt with implantation of cartilaginous or periosteal tissue in defects in the growth plate. In Wirth's study 10 sheep tibia were used. A comparison of both materials favoured cartilaginous tissue over periost which led to quick growth of bone formations.…”

Section: Discussionmentioning

confidence: 99%

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

Plánka¹,

Srnec²,

Raušer³

et al. 2012

BIOMED PAP

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Introduction. This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. Material and methods. The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Results. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. Conclusions. The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.

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“…The height of the growth plate adjacent to the defect was compared to an area of the growth plate towards the proximal side of the limb. Our laboratory has previously reported that this region is comparable with the growth plate from the unoperated limb [7,8,35]. Therefore, this region was used to represent a normal growth plate in this study.…”

Section: Chondrogenic Response Of the Growth Platementioning

confidence: 99%

The effect of recombinant human osteogenic protein-1 on growth plate repair in a sheep model

Thomas

Byers

Johnstone

et al. 2005

Journal of Orthopaedic Research

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Injuries to the growth plate in children can result in bone bridge formation, which ultimately lead to limb length and angular deformities. The histological and molecular changes associated with growth plate repair following the Langenskiöld procedure, a surgical technique used to remove impeding bone bridges, in conjunction with administration of recombinant human osteogenic protein-1 (rhOP-1) were examined using a sheep model. Following treatment with rhOP-1 there was an increase in the height of the growth plate immediately adjacent to the defect compared to untreated animals. The expression of type I collagen, osteopontin and decorin were observed in the growth plate adjacent to the defect in the untreated animals at day 56, but this response was accelerated in the rhOP-1 treated animals, with these molecules seen as early as day 7. Therefore, treatment with rhOP-1 initiated a complex response that was both chondrogenic and osteogenic in nature.

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The implantation of cartilaginous and periosteal tissue into growth plate defects

Cited by 37 publications

References 33 publications

Application of Autologous Bone Marrow Derived Mesenchymal Stem Cells to an Ovine Model of Growth Plate Cartilage Injury

Application of Autologous Bone Marrow Derived Mesenchymal Stem Cells to an Ovine Model of Growth Plate Cartilage Injury

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

The effect of recombinant human osteogenic protein-1 on growth plate repair in a sheep model

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