The Implication of microRNAs as non-invasive biomarkers in 179 Egyptian breast cancer female patients

SHAABAN, NADIA Z.; Ibrahim, Nashwa K; Saada, Helen N.; El‐Rashidy, Fatma H.; SHAABAN, HEBATALLAH M.; ELBAKARY, NERMEEN M.; Kodous, Ahmad S.

doi:10.32604/or.2022.027277

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…The LPS-BJ-1 ΔΔCt was calculated by subtracting the ΔCt of the interested gene (BJ-1 cells before LPS treated) from the ΔCt of each interested gene (BJ-1 cells post LPS treated). The PVA/AH LPS-BJ-1 ΔΔCt was calculated by subtracting the ΔCt of the interested gene (LPS-BJ-1 cells before PVA/AH treated) from the ΔCt of each interested gene (LPS-BJ-1 cells post PVA/AH treated).The formula 2 −ΔΔCt was used to get the fold change ( 39 – 42 ).…”

Section: Methodsmentioning

confidence: 99%

Hesperidin - loaded PVA/alginate hydrogel: targeting NFκB/iNOS/COX-2/TNF-α inflammatory signaling pathway

Kodous,

Abdel-Maksoud,

El-Tayeb

et al. 2024

Front. Immunol.

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IntroductionSkin injuries represent a prevalent form of physical trauma, necessitating effective therapeutic strategies to expedite the wound healing process. Hesperidin, a bioflavonoid naturally occurring in citrus fruits, exhibits a range of pharmacological attributes, including antimicrobial, antioxidant, anti-inflammatory, anticoagulant, and analgesic properties. The main objective of the study was to formulate a hydrogel with the intention of addressing skin conditions, particularly wound healing.MethodsThis research introduces a methodology for the fabrication of a membrane composed of a Polyvinyl alcohol - Sodium Alginate (PVA/A) blend, along with the inclusion of an anti-inflammatory agent, Hesperidin (H), which exhibits promising wound healing capabilities. A uniform layer of a homogeneous solution comprising PVA/A was cast. The process of crosslinking and the enhancement of hydrogel characteristics were achieved through the application of gamma irradiation at a dosage of 30 kGy. The membrane was immersed in a Hesperidin (H) solution, facilitating the permeation and absorption of the drug. The resultant system is designed to deliver H in a controlled and sustained manner, which is crucial for promoting efficient wound healing. The obtained PVA/AH hydrogel was evaluated for cytotoxicity, antioxidant and free radical scavenging activities, anti-inflammatory and membrane stability effect. In addition, its action on oxidative stress, and inflammatory markers was evaluated on BJ-1 human normal skin cell line.Results and DiscussionWe determined the effect of radical scavenging activity PVA/A (49 %) and PVA/AH (87%), the inhibition of Human red blood cell membrane hemolysis by PVA/AH (81.97 and 84.34 %), hypotonicity (83.68 and 76.48 %) and protein denaturation (83.17 and 85.8 %) as compared to 250 μg/ml diclofenac (Dic.) and aspirin (Asp.), respectively. Furthermore, gene expression analysis revealed an increased expression of genes associated with anti-oxidant and anti-inflammatory properties and downregulated TNFα, NFκB, iNOS, and COX2 by 67, 52, 58 and 60%, respectively, by PVA/AH hydrogel compared to LPS-stimulated BJ-1 cells. The advantages associated with Hesperidin can be ascribed to its antioxidant and anti-inflammatory attributes. The incorporation of Hesperidin into hydrogels offers promise for the development of a novel, secure, and efficient strategy for wound healing. This innovative approach holds potential as a solution for wound healing, capitalizing on the collaborative qualities of PVA/AH and gamma irradiation, which can be combined to establish a drug delivery platform for Hesperidin.

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Section: Methodsmentioning

confidence: 99%

Hesperidin - loaded PVA/alginate hydrogel: targeting NFκB/iNOS/COX-2/TNF-α inflammatory signaling pathway

Kodous,

Abdel-Maksoud,

El-Tayeb

et al. 2024

Front. Immunol.

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“…The analysis included examining the variations in mRNA target content concerning the glyceraldehyde-3-phosphate dehydrogenase gene, which was normalized. This analysis was conducted using the comparative cycle threshold approach designated by Livak and Schmittgen [38][39][40][41] .…”

Section: Real-time Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Gamma radiation assisted green synthesis of hesperidin-reduced graphene oxide nanocomposite targeted JNK/SMAD4/MMP2 signaling pathway

Kodous,

Taha,

El-Maghraby

et al. 2024

Sci Rep

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In this study, a novel method for the fabrication of hesperidin/reduced graphene oxide nanocomposite (RGOH) with the assistance of gamma rays is reported. The different RGOHs were obtained by varying hesperidin concentrations (25, 50, 100, and 200 wt.%) in graphene oxide (GO) solution. Hesperidin concentrations (25, 50, 100, and 200 wt.%) in graphene oxide (GO) were varied to produce the various RGOHs. Upon irradiation with 80 kGy from γ-Ray, the successful reduction of GO occurred in the presence of hesperidin. The reduction process was confirmed by different characterization techniques such as FTIR, XRD, HRTEM, and Raman Spectroscopy. A cytotoxicity study using the MTT method was performed to evaluate the cytotoxic-anticancer effects of arbitrary RGOH on Wi38, CaCo2, and HepG2 cell lines. The assessment of RGOH’s anti-inflammatory activity, including the monitoring of IL-1B and IL-6 activities as well as NF-kB gene expression was done. In addition, the anti-invasive and antimetastatic properties of RGOH, ICAM, and VCAM were assessed. Additionally, the expression of the MMP2-9 gene was quantified. The assessment of apoptotic activity was conducted by the detection of gene expressions related to BCl2 and P53. The documentation of the JNK/SMAD4/MMP2 signaling pathway was ultimately accomplished. The findings of our study indicate that RGOH therapy has significant inhibitory effects on the JNK/SMAD4/MMP2 pathway. This suggests that it could be a potential therapeutic option for cancer.

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“…The results of the statistical analysis showed in figure (1) that there are significant differences in the toxicity of the plant extracts according the type of plant extract used. It was observed that the mortality percentage increase with increasing concentration and that there is a difference between the plants in the occurrence of the deadly effect so that the plant extract exceed to another , In the quality and quantity of the active compounds that are contained in different plants which affect in the nervous system of the insect, which paralyzes the movement leading to shock and death, as well as the impact on the work of enzymes necessary for the vital processes, which lead to the cessation of metabolism and thus Death [10]. The results of the table (1) showed that the extract of the seeds of the Helianthus annuus L. gave the highest effect in mortality from the rest of the plant…”

Section: -The Efficacy Of Plant Extracts In Tribolium Castaneum After...mentioning

confidence: 99%

Compar the Effectiveness of Some Plant Extracts and Nd: YAG Laser in the Tribolium castaneum

Mahdi¹,

Lafta²,

Taha³

2023

Tikrit J. Pure Sci.

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The present study was conducted to evaluate the efficiency and effectiveness of some plant extracts of Helianthus annuus L., Sesbania sesban and Olea europea concentrations 100, 500, 1000, 5000 ppm. and the effect of Nd: YAG laser with wavelength of( 1064nm) and different energies which conducted at (10,15 and 20) within (5 pulses/sec) to destruction of the Tribolium castaneum, the study found different results according to the different concentrations used, time period and the different energies of the laser during the treatment. Treatment with plant extracts The extract of the Helianthus annuus L. was hightst to the rest of the plant extracts. It gave a 100% mortality rate at 5000 ppm concentration 72 hours of treatment. Then the seesban seed extract came in terms of effect, giving 96.6% mortality rate at the same concentration after 72 hours. While the effect of Olea europea extract was the last impact"on the insect, where the rate of mortality 86.5% in the same concentration also and within ( 72 hours ) of treatment. The results showed that the effect of laser radiation on the life of the insect, where it gave the highest mortality rate 73.6% when irradiation at the highest power of 420mj and exposure time 20Sec. the results were obtained after observation of the effect of plant extracts and Nd : YAG at different time intervals 24, 48 hours and 72 hours after the treatment of the Tribolium castaneum .

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The Implication of microRNAs as non-invasive biomarkers in 179 Egyptian breast cancer female patients

Cited by 6 publications

References 37 publications

Hesperidin - loaded PVA/alginate hydrogel: targeting NFκB/iNOS/COX-2/TNF-α inflammatory signaling pathway

Hesperidin - loaded PVA/alginate hydrogel: targeting NFκB/iNOS/COX-2/TNF-α inflammatory signaling pathway

Gamma radiation assisted green synthesis of hesperidin-reduced graphene oxide nanocomposite targeted JNK/SMAD4/MMP2 signaling pathway

Compar the Effectiveness of Some Plant Extracts and Nd: YAG Laser in the Tribolium castaneum

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