Manufacturing enterprises are more and more aware of establishing collaborative relationships with the network partners, due to the advantages associated. CN consist of autonomous partners, each one defining its own objectives and formulating its own strategies. The strategies diversity may result in conflict situations, among the enterprises of the same CN, since contradictions between the strategies formulated might emerge. These contradictions appear when the strategies activated in one enterprise negatively influence the objectives defined by other enterprises of the network. The lack of coherence and concordance among the formulated strategies leads to its misalignment. The connotations derived from the strategies misalignments affect the achievement of enterprises objectives, reducing their performance levels, and influencing on the wellbeing of the collaborative relationships established. If the conflicts that arise, derived from the lack of strategies alignment, remain on time and are not tackled, the strategies misalignment could lead, in the long term, to the breakdown of the CN. The success of obtaining higher levels performance in the CN is directly related with the activation of a proper combination of strategies in each enterprise belonging to the network. This thesis proposes a complete approach, consisting of a model, a method, a guideline and a set of tools, used (i) to identify the degree of alignment of the strategies, from a holistic perspective, and (ii) to propose the activation of the aligned strategies.The main aim of this thesis is to provide the enterprises appropriate mechanisms to remove the strategies misalignment problem, in order to establish long-term collaborative relationships. The proposed solution is based on a mathematical model, which allows to formally modeling the strategies alignment process, solving it through systems dynamic (SD) method. SD allows representing causal relationships between the strategies and the objectives achievement, within the complex system formed by the enterprises of a CN. A performance measurement scheme is provided to quantitatively measure the influences between the strategies and the objectives. Moreover, a simulation tool is used to automatically solve, in a computer program, the proposed model, assessing and supporting the strategies alignment process.The contribution of this thesis has been validated in two industrial pilots belonging to the food and automotive industry.