The Importance of Bacterial Culture to Food Microbiology in the Age of Genomics

Gill, Alexander

doi:10.3389/fmicb.2017.00777

Cited by 53 publications

(32 citation statements)

References 52 publications

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“…Therefore, a sample preparation method was designed to include a pre-enrichment step, allowing the effective detection of 1-5 CFU of Staphylococcus in 10 mL of blood. The enrichment procedure also assures the detection of viable cells 66 , preventing the occurrence of false positives. Moreover, the developed method avoided the occurrence of positive signals derived from the autofluorescence of blood components 26,43,67 and revealed a good analytical reproducibility with results that were easily and objectively interpreted.…”

Section: Discussionmentioning

confidence: 99%

A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood

Costa

Dias

Melo

et al. 2020

Sci Rep

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Bloodstream infections (BSIs) are considered a major cause of death worldwide. Staphylococcus spp. are one of the most BSIs prevalent bacteria, classified as high priority due to the increasing multidrug resistant strains. Thus, a fast, specific and sensitive method for detection of these pathogens is of extreme importance. In this study, we have designed a novel assay for detection of Staphylococcus in blood culture samples, which combines the advantages of a phage endolysin cell wall binding domain (CBD) as a specific probe with the accuracy and high-throughput of flow cytometry techniques. In order to select the biorecognition molecule, three different truncations of the C-terminus of Staphylococcus phage endolysin E-LM12, namely the amidase (AMI), SH3 and amidase+SH3 (AMI_SH3) were cloned fused with a green fluorescent protein. From these, a higher binding efficiency to Staphylococcus cells was observed for AMI_SH3, indicating that the amidase domain possibly contributes to a more efficient binding of the SH3 domain. The novel phage endolysin-based flow cytometry assay provided highly reliable and specific detection of 1-5 CFU of Staphylococcus in 10 mL of spiked blood, after 16 hours of enrichment culture. Overall, the method developed herein presents advantages over the standard BSIs diagnostic methods, potentially contributing to an early and effective treatment of BSIs. Bloodstream infections (BSIs) are severe diseases caused by the presence of microorganisms, mainly bacteria, in blood and are characterized by high morbidity and mortality 1,2. The BSIs and associated organ dysfunctions (sepsis or septic shock) remain a life-threating disease due, partially, to the inability to rapidly detect and identify the responsible pathogens 3,4. Staphylococcus spp. are Gram-positive facultative anaerobic bacteria that frequently colonize the human body 5,6. These pathogens are becoming increasingly resistant to antibiotics and are well-established in both community and healthcare environments, being commonly isolated in intensive care units (ICU) 6,7. Staphylococcus aureus is a common cause of a variety of infections, from superficial skin infections to life-threatening diseases, including necrotizing pneumonia 8 , infective endocarditis 9 and BSIs 10. Coagulase-negative staphylococci (CoNS) have also been described as harmful to humans, causing several infections, particularly in patients with implanted medical devices 6. The empirical antibiotic therapy remains the standard of BSIs treatments 11 and its correct use within the first hour after the recognition of the BSI is recommended by the Surviving Sepsis Campaign Guidelines 11 and was reported as having a great impact on the patient survival rate 12. Nevertheless, the extensive use of broad-spectrum antibiotics and the large number of patients having negative blood culture samples and thus receiving unnecessary antibiotic treatment, are important contributors to the increase of antimicrobial resistance 13-15. Thus, sensitive, rapid, cost-efficient and specific ...

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Section: Discussionmentioning

confidence: 99%

A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood

Costa

Dias

Melo

et al. 2020

Sci Rep

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“…Regarding the high spread of foodborne pathogens illness, the inspection regulations are very strict with the requirements for process control. The LOD for food pathogens is restricted to 1 cell per unit of food sample [25]. Depending on the target pathogen and the food sample, the analytical unit may be considered from 25 to 325 g.…”

Section: Conventional Methodsmentioning

confidence: 99%

Foodborne Pathogens Detection: Persevering Worldwide Challenge

Baraketi¹,

Salmieri²,

Lacroix³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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According to Health Canada, foodborne disease is responsible of more than 4 million cases per year. In United States, more than 48 million people get sick, 128,000 are hospitalized and 3000 die every year in United States due to foodborne diseases according to the Center for Disease Control and Prevention. Cross-contamination from the raw materials, during the process or on working surface has to be rapidly detected. Good manufacturing practices (GMP) and hazard analysis critical control point (HACCP) can help to reduce the incidence of contamination. However, the development of sensitive and rapid methods of detection is still an important need. Standard culture-based methods request the consumption of large amounts of media, are time-consuming and interferences can occur when samplings are done in complex food matrices. The polymerase chain reaction (PCR)-based methods are new technologies. These methods show high level of specificity and sensitivity because they can detect nucleic acid sequences of target bacteria. However, they require an expensive instrumentation and trained scientific technicians. This review is focusing on the development of new simple, sensitive, specific, and time-saving technologies in order to detect quickly foodborne pathogens for application in food industries.

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“…The bacteria of interest to food microbiology can be divided into infectious agents, causes of food borne intoxication, spoilage and processing aids. Food biotechnology is mainly concerned with application of live microorganisms like yeasts, fungi, bacilli and lactic acid bacteria in industrial processes (Gill 2017). There is a huge influence of food microbes on human health, for example, in pre-and probiotics research.…”

Section: Genomics In Food Microbiologymentioning

confidence: 99%

Role of genome technology in dairy food quality and safety

De¹

2019

IJDS

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Genomics tools, specifically the different affordable Sequencing methods, are rapidly transforming almost every aspect of Biological Sciences including food science. Applications of genomic technologies in food sciences is generally considered a great leap forward with some concerns regarding the need of well-equipped and trained laboratories to discriminate between good and bad microorganisms in the fermented food stuff. Species identification of raw and finished dairy and animal products has become an important issue regarding the assessment of food composition. Protection against species substitution or admixture in dairy and animal products is of significant importance for reasons relating to consumer preference, public health and government regulations. Milk and milk products are of particular interest, because they are a group of foods that play an important role in feeding the population and are essential for certain groups of consumers (women, children, and the elderly). Milk products are often prone to adulteration practices, since milk is a fairly expensive raw material and can be easily replaced in part by other dairy or non-dairy ingredients. DNA based methods became very popular, since they are considered more reliable as a result of DNA stability under high temperatures, pressures and chemical treatments used during processing of food products. The polymerase chain reaction (PCR) is the most widely used molecular technique, because of its simplicity, sensitivity and reproducibility. Ruminant milk can be easily used as a source of DNA, since it has a large amount of somatic cells, mostly leucocytes but also epithelial cells from the milking mother, which contain genomic DNA suitable for any kind of genomic DNA testing. PCR amplification of various regions of mitochondrial genome, 12S rRNA, growth hormone (GH) gene and PCR RFLP have been reported and proved the sensitivity of DNA based methods and their reliability for species identification in a wide range of raw and finished animal food products. Complete genome sequencing of bacterial isolates (pathogenic or lactic acid bacteria) has started to replace contemporary popular molecular methods, like ribotyping, MLST and Pulse Field Gel Electrophoresis (PFGE), as subtyping method of choice. Whole Genome Sequencing can able to route of transmission in case of increasingly smaller outbreaks.

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The Importance of Bacterial Culture to Food Microbiology in the Age of Genomics

Cited by 53 publications

References 52 publications

A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood

A novel flow cytometry assay based on bacteriophage-derived proteins for Staphylococcus detection in blood

Foodborne Pathogens Detection: Persevering Worldwide Challenge

Role of genome technology in dairy food quality and safety

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