In the context of sustainable development, the impact of corporate culture on employee well-being is particularly significant. This paper examines why corporate culture is a key factor in enhancing employee well-being and driving corporate sustainability. Research shows that when corporate culture emphasizes respect, inclusiveness, justice and teamwork, it can significantly enhance the enthusiasm and creativity of employees, thus promoting the innovation and long-term development of enterprises. In a diverse work environment, building a supportive and inclusive corporate culture is essential to accommodate the needs of employees from different backgrounds. This will not only help improve the job satisfaction of employees, but also enhance the social responsibility and market competitiveness of enterprises. The paper also analyses the role of corporate culture in responding to modern workplace challenges such as remote working and work-life balance. Flexible work arrangements, employee health and welfare programs, and continuous career development opportunities were identified as key to enhancing employee well-being. These measures not only improve the quality of life of employees, but also enhance their loyalty and sense of belonging to the enterprise. A positive, inclusive and supportive corporate culture is essential to improve employee job satisfaction and loyalty and promote the long-term beneficial development of the business.