The Importance of Factor XIII as a Component of Fibrin Sealants

Dickneite, Gerhard; Metzner, Hubert J.; Kroez, Monika; Hein, B; Nicolay, Uwe

doi:10.1006/jsre.2002.6495

Cited by 28 publications

(20 citation statements)

References 18 publications

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“…F-XIII, when added to fibrin sealant, improves fibrin cross-linking, making the clot more resistant to lysis by plasmin, and increases the strength of the clot, making it more resistant to mechanical damage [21]. We observed similar effects in F-XIII animals on day 5 and 7.…”

Section: Discussionsupporting

confidence: 71%

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Hasegawa¹,

Eguchi

Endoh³

et al. 2004

Eur Surg Res

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Background: Blood coagulation factor XIII (F-XIII) promotes cross-linking of fibrin during blood coagulation. Impaired clot stabilization in patients with genetic deficiencies of F-XIII is associated with marked pathologies of wound healing. Methods: 60 rats given carbon tetrachloride underwent ileal anastomosis after which they received albumin (Alb animals) or F-XIII concentrate (F-XIII animals) immediately after surgery and daily thereafter until day 2 or 4 and were euthanized on day 3 or 5, respectively, or until day 5 and were euthanized on day 7, 10, or 21. We measured the plasma F-XIII activity and anastomotic tensile strength, followed by immunohistochemical localization of F-XIII subunit A within anastomoses. Results: On day 3, there were no significant differences between Alb and F-XIII animals for plasma F-XIII activity and tensile strength, and both groups of animals showed little immunostaining for F-XIII on anastomoses. Plasma F-XIII activities did not differ between Alb and F-XIII animals on day 5 (115.8 ± 16.8 vs. 137.3 ± 14.9%). Although the tensile strength in both groups was increased compared with that of day 3, that in F-XIII animals (129.8 ± 3.3 gf) was significantly higher than that in Alb animals (100.8 ± 5.3 gf, p = 0.014). F-XIII animals showed de novo collagen fibers and intense immunoreactivity of F-XIII in the extracellular matrix around the anastomoses. Similar differences occurred on day 7 but not days 10 and 21. Conclusions: F-XIII concentrate may accelerate the early healing process of intestinal anastomosis because of the protein’s accumulation in situ.

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Section: Discussionsupporting

confidence: 71%

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Hasegawa¹,

Eguchi

Endoh³

et al. 2004

Eur Surg Res

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“…It is known, for example, that a high salt concentration can inhibit fibroblast proliferation and that FXIII aids the infiltration of fibroblasts 17 . Previous investigations have identified that adequate clotable protein content and effective mixing of the fibrinogen and thrombin com‐ ponents of the FS are prerequisites for successful early hemostasis, and that FXIII activity is key to preventing rebleeding and premature clot lysis 18,19 . Although hemostasis must remain of primary importance, investigation of the effects of formulation differences on FS degradation and associated healing would be an interesting extension to the present study.…”

Section: Discussionmentioning

confidence: 83%

Wound healing and degradation of the fibrin sealant Beriplast^® P following partial liver resection in rabbits

Kroez

Lang

Dickneite

2005

Wound Repair Regeneration

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The objective of this study was to investigate the degradation kinetics of the fibrin sealant (FS) Beriplast P in an experimental liver surgery model in rabbits. A partial liver resection was performed in 21 rabbits, and the wound area covered with Beriplast P to ensure hemostasis. Wound healing of the resection sites was evaluated morphologically over 11 weeks. Degradation of the FS was evaluated by measuring the thickness of the remaining fibrin layer. Plasma samples were analyzed for antibodies against fibrinogen, albumin, thrombin, fibrin, and factor XIII. No postoperative hemorrhage was observed, indicating successful hemostasis throughout. The FS was degraded with a half-life of about 25 days postapplication and was completely replaced by granulation tissue within 9 weeks. The FS degradation and tissue development followed the general stages of wound healing: inflammation and resorption, proliferation, organization and production of collagen, maturation, and scarring. An immune reaction was elicited against the main four human proteins of the FS. The antibody titers peaked on day 14, with a gradual decrease thereafter. We conclude that the FS accomplished hemostasis, facilitated healing in accordance with natural processes, and was completely degraded over time. In humans, the reduced immunogenicity of the FS would potentially increase its degradation half-life.

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“…In fact, several studies in animals and humans have supported the use of fibrin-based materials for adhesion prevention [50,51].…”

Section: Discussionmentioning

confidence: 98%

The use of novel hemostatic sealant (Tisseel®) in laparoscopic myomectomy: a case–control study

et al. 2012

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The Importance of Factor XIII as a Component of Fibrin Sealants

Cited by 28 publications

References 18 publications

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Wound healing and degradation of the fibrin sealant Beriplast^® P following partial liver resection in rabbits

The use of novel hemostatic sealant (Tisseel®) in laparoscopic myomectomy: a case–control study

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The Importance of Factor XIII as a Component of Fibrin Sealants

Cited by 28 publications

References 18 publications

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Effectiveness of Coagulation Factor XIII Concentrate for Reversing Loss of Tensile Strength of Rat Intestinal Anastomoses

Wound healing and degradation of the fibrin sealant Beriplast® P following partial liver resection in rabbits

The use of novel hemostatic sealant (Tisseel®) in laparoscopic myomectomy: a case–control study

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Wound healing and degradation of the fibrin sealant Beriplast^® P following partial liver resection in rabbits