The importance of Ile716 toward the mutagenicity of 8-Oxo-2’-deoxyguanosine with Bacillus fragment DNA polymerase

Bst polymerase — a humble relative of Taq polymerase

“…Plausibly, the mutated residue does not impede the entrance of the incoming nucleotide in the insertion site. The I716A substitution led to a 30-fold decrease in activity, reduced fidelity, and denaturation after incubation at 65 °C [97] . Thus, mutations of catalytically active residues were deleterious, while the changes in the preinsertion site were not fatal.…”