The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence

Ge, Heng; Tollner, Theodore L.; Hu, Zhen; Dai, Mimi; Li, Xiaohe; Guan, Heqin; Shan, Dan; Zhang, Xiuju; Lv, Jieqiang; Huang, Changjiang; Dong, Qiaoxiang

doi:10.1002/mrd.22042

Cited by 124 publications

(96 citation statements)

References 45 publications

(66 reference statements)

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“…It has been proposed that development is affected by lower numbers of mitochondria or at least of copies of mtDNA (Ge et al 2012). However, vitamin K 2 was shown not to have any impact on the mtDNA/nDNA ratio (Rao et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

et al. 2014

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Mitochondria play an important role during early development in mammalian embryos. It has been shown that properly controlled follicular preparation increases the likelihood of in-vitro-produced bovine embryos reaching the blastocyst stage and that competent embryos exhibit heightened expression of genes associated with mitochondrial function. We hypothesized that apparently incompetent embryos could be rescued by restoring mitochondrial function. It has been shown that vitamin K 2 (a membrane-bound electron carrier similar to ubiquinone) can restore mitochondrial dysfunction in eukaryotic cells. The aim of this study was therefore to investigate the effects of vitamin K 2 on bovine embryonic development in vitro. The vitamin was found most effective when added 72 h after fertilization. It produced a significant (P!0.05) increase in the percentage of blastocysts (C8.6%), more expanded blastocysts (C7.8%), and embryos of better morphological quality. It improved the mitochondrial activity significantly and had a measurable impact on gene expression. This is the first demonstration that current standard conditions of in vitro production of bovine embryos may be inadequate due to the lack of support for mitochondrial function and may be improved significantly by supplementing the culture medium with vitamin K 2 .

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Section: Discussionmentioning

confidence: 99%

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

et al. 2014

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“…In addition, centrifugation followed by the removal of the enriched mitochondrial fraction did not affect the developmental competence of embryos [8]. Conversely, reducing the mtDNA number using DCC during IVM resulted in a low development ratio of blastocysts [27]. In experiment 1, the mtDNA number in noncleaved oocytes was the lowest in the 2- to 3-cell, 4- to 7-cell, and >7-cell stage embryos, although the mtDNA number did not differ among the 3 stages.…”

Section: Discussionmentioning

confidence: 99%

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Takeo

Goto

Kuwayama

et al. 2013

Journal of Reproduction and Development

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Age-associated deterioration in both the quality and quantity of mitochondria occurs in older women. The main aim of this study was to examine the effect of age on mitochondrial DNA copy number (mtDNA number) in early developmental stage bovine embryos as well as the dynamics of mtDNA number during early embryo development. Real-time PCR was used to determine mtDNA number. In vitro-produced embryos 48 h after insemination derived from Japanese black cows, ranging in age from 25 to 209 months were categorized based on their cleavage status. There was an overall negative relationship between the age of the cow and cleavage status, to the extent that the ratio of embryos cleaved over the 4-cell stage was greater in younger cows. The mtDNA number did not differ among the cleaved status of embryos. In the next experiment, oocytes collected from each donor cow were divided into 2 groups containing 10 oocytes each, in order to compare the mtDNA number of mature oocytes and early developmental stage embryos within individuals. Upon comparing the mtDNA number between oocytes at the M2 stage and early developmental stage 48 h post insemination, mtDNA number was found to decrease in most cows, but was found to increase in some cows. In conclusion, age affects the cleaving ability of oocytes, and very old cows (> 180 months) tend to have lower mtDNA numbers in their oocytes. The change in mtDNA number during early development varied among individual cows, although overall, it showed a tendency to decrease.

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“…These observations suggest that mitochondrial damage caused by CCCP treatment is eliminated by the proteasome and that these mitochondria are replaced by de novo-synthesised mitochondria during oocyte maturation. Ge et al (2012) reported that FCCP treatment of oocytes decreases maturation ability and spindle stability but does not alter the Mt number. We speculate that the reported result may be due to the presence of both pathways of mitochondrial biogenesis and degradation.…”

Section: Mitochondrial Impairments and Kineticsmentioning

confidence: 99%

Mitochondrial biogenesis and degradation are induced by CCCP treatment of porcine oocytes

et al. 2015

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In this study, we investigated the mitochondrial quality control system in porcine oocytes during meiotic maturation. Cumulus cell oocyte complexes (COCs) collected from gilt ovaries were treated with 10 mM carbonyl cyanide-m-chlorophenylhydrazone (CCCP; a mitochondrial uncoupler) for 2 h. The CCCP treatment was found to significantly reduce ATP content, increase the amount of phosphorylated AMP-activated protein kinase and elevate reactive oxygen species levels in oocytes. When the CCCP-treated COCs were cultured further for 44 h in maturation medium, the ATP levels were restored and the parthenogenetic developmental rate of oocytes to the blastocyst stage was comparable with that of untreated COCs. To examine the effects of CCCP treatment of oocytes on the kinetics of mitochondrial DNA copy number (Mt number), COCs treated with 0 or 10 mM CCCP were cultured for 44 h, after which the Mt number was determined by RT-PCR. CCCP treatment was found to increase the Mt number in the modified maturation medium in which mitochondrial degradation was inhibited by MG132, whereas CCCP treatment did not affect the Mt number in the maturation medium lacking MG132. The relative gene expression of TFAM was furthermore shown to be significantly higher in CCCP-treated oocytes than in untreated oocytes. Taken together, the finding presented here suggest that when the mitochondria are injured, mitochondrial biogenesis and degradation are induced, and that these processes may contribute to the recuperation of oocytes.Reproduction (2015) 150 97-104

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The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence

Cited by 124 publications

References 45 publications

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

Effect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine Embryos

Mitochondrial biogenesis and degradation are induced by CCCP treatment of porcine oocytes

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