The Importance of Perfusate Content of Free Fatty Acids for Dog Kidney Preservation

Slaattelid, O.; Flatmark, A; Skrede, Sverre

doi:10.1080/00365517609055254

Cited by 3 publications

(8 citation statements)

References 22 publications

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“…As can be seen from table IV the lactate concen tration increased during the perfusion period. The average fall in FFA concentration, 1,000 //mol/l, was the same whether the perfusate con tained glucose or not and was equal to that observed in previous experi ments [19].…”

Section: Perfusate Analysessupporting

confidence: 58%

“…prior to nephrecto my. After perfusion for 48 h the kidneys were reimplanted to the neck [19] of the original donor. Contralateral nephrectomy was performed simultaneously.…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Perfusate FFA was measured before and after each kidney perfusion. The analytical methods have been described previously [19]. All perfusates were tested for sterility, and bacteriological contamination was never found.…”

Section: Sampling and Analyses Of Perfusatesmentioning

confidence: 99%

“…In a previous study [19] it was shown that free fatty acids (FFA) are utilized by the dog kidney during hypothermic perfusion. The functional quality of perfused kidneys depended on the content of FFA in the per fusate.…”

Section: Introductionmentioning

confidence: 99%

“…Initial flushing of the excised kidney was performed as previously described [19]. The kidneys were preserved in the Gambro perfusion machine.…”

Section: Kidney Preservationmentioning

confidence: 99%

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Preservation of Dog Kidneys by Hypothermic Perfusion with Glucose-Free and Glucose-Rich Perfusates

Slaattelid

Grønnerød

Horn³

1976

Eur Surg Res

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Dog kidneys were perfused for 48 h at 8–10°C with two different perfusion fluids: one fluid rich in glucose, and another in which mannitol was a substitute for glucose. Immediately after reimplantation of the perfused kidneys, contralateral nephrectomy was performed. Kidney function, estimated by serum creatinine, was the same in both groups of animals, and 2 weeks after implantation it was identical to that of dogs with a single normal kidney. In a second series of experiments the renal utilization of glucose and glycogen was studied. Although small amounts of perfusate glucose were metabolized to lactate and CO₂, the results of this study indicate that the kidney is not dependent upon glucose for its metabolic needs in order to retain function during 48 h hypothermic preservation.

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Section: Perfusate Analysessupporting

confidence: 58%

“…prior to nephrecto my. After perfusion for 48 h the kidneys were reimplanted to the neck [19] of the original donor. Contralateral nephrectomy was performed simultaneously.…”

Section: In Vivo Studiesmentioning

confidence: 99%

Section: Sampling and Analyses Of Perfusatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Initial flushing of the excised kidney was performed as previously described [19]. The kidneys were preserved in the Gambro perfusion machine.…”

Section: Kidney Preservationmentioning

confidence: 99%

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Preservation of Dog Kidneys by Hypothermic Perfusion with Glucose-Free and Glucose-Rich Perfusates

Slaattelid

Grønnerød

Horn³

1976

Eur Surg Res

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Evaluation of Kidney Viability during Hypothermic Perfusion or Cold Storage

Nauclér¹,

Bylund-Fellenius²,

Jonsson³

et al. 1984

Eur Surg Res

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The effect of up to 6 days of hypothermic perfusion and up to 72 h of cold storage on tissue viability were studied from measurements of sodium potassium pump activity and adenosine nucleotide content. For the hypothermic perfusion two different albumin-based perfusates were used, one with, the other without addition of caprylic acid and amino acids. No difference could be found in the parameters studied between perfusion with these two perfusates. For both perfusates total adenine nucleotide content (TAN) decreased by approximately 60% during perfusion while energy charge potential (ECP) and the ATP/ADP ratio remained constant. During cold storage a decrease of both TAN and ECP and the ratio ATP/ADP was seen, the main decrease occurring within the 1 st h. The transmembrane potassium influx as deduced from potassium content in incubated cortical slices during steady state conditions decreased during 6 days of hypothermic storage to 24.5 ± 2.4 µmol/kg which was a significantly higher level than after 24 h of cold storage. Thus, addition of extra substrate to the perfusate does not seem to improve kidney cortex viability during hypothermic perfusion. Parenchymal viability is better preserved with continuous hypothermic perfusion, compared to simple cold storage in a solution of the same ionic composition as that used for perfusion.

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Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review

Verstraeten,

Den abt,

Ghesquière

et al. 2023

JCM

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This scoping review summarizes what is known about kidney metabolism during hypothermic perfusion preservation. Papers studying kidney metabolism during hypothermic (<12 °C) perfusion were identified (PubMed, Embase, Web of Science, Cochrane). Out of 14,335 initially identified records, 52 were included [dog (26/52), rabbit (2/52), pig (20/52), human (7/52)]. These were published between 1970–2023, partially explaining study heterogeneity. There is a considerable risk of bias in the reported studies. Studies used different perfusates, oxygenation levels, kidney injury levels, and devices and reported on perfusate and tissue metabolites. In 11 papers, (non)radioactively labeled metabolites (tracers) were used to study metabolic pathways. Together these studies show that kidneys are metabolically active during hypothermic perfusion, regardless of the perfusion setting. Although tracers give us more insight into active metabolic pathways, kidney metabolism during hypothermic perfusion is incompletely understood. Metabolism is influenced by perfusate composition, oxygenation levels, and likely also by pre-existing ischemic injury. In the modern era, with increasing donations after circulatory death and the emergence of hypothermic oxygenated perfusion, the focus should be on understanding metabolic perturbations caused by pre-existing injury levels and the effect of perfusate oxygen levels. The use of tracers is indispensable to understanding the kidney’s metabolism during perfusion, given the complexity of interactions between different metabolites.

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The Importance of Perfusate Content of Free Fatty Acids for Dog Kidney Preservation

Cited by 3 publications

References 22 publications

Preservation of Dog Kidneys by Hypothermic Perfusion with Glucose-Free and Glucose-Rich Perfusates

Preservation of Dog Kidneys by Hypothermic Perfusion with Glucose-Free and Glucose-Rich Perfusates

Evaluation of Kidney Viability during Hypothermic Perfusion or Cold Storage

Current Insights into the Metabolome during Hypothermic Kidney Perfusion—A Scoping Review

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