The importance of proteins of the RNase II/RNB-family in pathogenic bacteria

Matos, Rute G.; Bárria, Cátia; Moreira, Ricardo Neves; Barahona, Susana; Domingues, Susana; Arraiano, Cecília M.

doi:10.3389/fcimb.2014.00068

Cited by 29 publications

(18 citation statements)

References 41 publications

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“…As part of an essential RNA-degrading multiprotein complex, RNase II is involved in the maturation, turnover, and quality control of RNA (77,78). For many pathogens, the activity of RNase II has been correlated with the regulation of virulence and motility (79,80). With Escherichia coli, the results from next generation sequencing show that RNase II mutants inhibited motility and increased biofilm production.…”

Section: Resultsmentioning

confidence: 91%

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Hurst¹,

Rowedder²,

Michaels³

et al. 2015

J. Bacteriol.

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The entomopathogenic nematode Heterorhabditis bacteriophora forms a specific mutualistic association with its bacterial partner Photorhabdus temperata. The microbial symbiont is required for nematode growth and development, and symbiont recognition is strain specific. The aim of this study was to sequence the genome of P. temperata and identify genes that plays a role in the pathogenesis of the Photorhabdus-Heterorhabditis symbiosis. A draft genome sequence of P. temperata strain NC19 was generated. The 5.2-Mb genome was organized into 17 scaffolds and contained 4,808 coding sequences (CDS). A genetic approach was also pursued to identify mutants with altered motility. A bank of 10,000 P. temperata transposon mutants was generated and screened for altered motility patterns. Five classes of motility mutants were identified: (i) nonmotile mutants, (ii) mutants with defective or aberrant swimming motility, (iii) mutant swimmers that do not require NaCl or KCl, (iv) hyperswimmer mutants that swim at an accelerated rate, and (v) hyperswarmer mutants that are able to swarm on the surface of 1.25% agar. The transposon insertion sites for these mutants were identified and used to investigate other physiological properties, including insect pathogenesis. The motility-defective mutant P13-7 had an insertion in the RNase II gene and showed reduced virulence and production of extracellular factors. Genetic complementation of this mutant restored wild-type activity. These results demonstrate a role for RNA turnover in insect pathogenesis and other physiological functions. IMPORTANCEThe relationship between Photorhabdus and entomopathogenic nematode Heterorhabditis represents a well-known mutualistic system that has potential as a biological control agent. The elucidation of the genome of the bacterial partner and role that RNase II plays in its life cycle has provided a greater understanding of Photorhabdus as both an insect pathogen and a nematode symbiont.

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Section: Resultsmentioning

confidence: 91%

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Hurst¹,

Rowedder²,

Michaels³

et al. 2015

J. Bacteriol.

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“…An encoded RNase II disrupted in JG574 is a member of the RNase II/RNB-family of 3′-5′ exoribonucleases, which function in mRNA turnover ( Table 1 ). Several enzymes from this group, particularly RNase R homologs, are implicated as virulence factors in various pathogens ( Matos et al, 2014 ). Interestingly, an RNAse II from the nematode symbiont Photorhabdus temperata is needed for full insect virulence but not symbiosis ( Hurst et al, 2015 ).…”

Section: Regulationmentioning

confidence: 99%

Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential

et al. 2016

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Digestive-tract microbiota exert tremendous influence over host health. Host-symbiont model systems are studied to investigate how symbioses are initiated and maintained, as well as to identify host processes affected by resident microbiota. The medicinal leech, Hirudo verbana, is an excellent model to address such questions owing to a microbiome that is consistently dominated by two species, Aeromonas veronii and Mucinivorans hirudinis, both of which are cultivable and have sequenced genomes. This review outlines current knowledge about the dynamics of the H. verbana microbiome. We discuss in depth the factors required for A. veronii colonization and proliferation in the leech crop and summarize the current understanding of interactions between A. veronii and its annelid host. Lastly, we discuss leech usage in modern medicine and highlight how leech-therapy associated infections, often attributable to Aeromonas spp., are of growing clinical concern due in part to an increased prevalence of fluoroquinolone resistant strains.

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“…For many pathogens, this RNase activity has been linked to virulence and pathogenesis (Arraiano et al 2010a;Matos et al 2014;Tsao et al 2009).…”

Section: Rna Turnover May Be Involved In Regulation Of Pathogenesismentioning

confidence: 99%

Identification and characterization of Photorhabdus temperata mutants altered in hemolysis and virulence

Chapman

Tisa

2016

Can. J. Microbiol.

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Photorhabdus temperata is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora and an insect pathogen. This bacterium produces a wide variety of virulence factors and hemolytic activity. The goal of this study was to identify hemolysin-defective mutants and test their virulence. A genetic approach was used to identify mutants with altered hemolytic activity by screening a library of 10 000 P. temperata transposon mutants. Three classes of mutants were identified: (i) defective (no hemolytic activity), (ii) delayed (delayed initiation of hemolytic activity), and (iii) early (early initiation of hemolytic activity). The transposon insertion sites for these mutants were identified and used to investigate other physiological properties, including insect pathogenesis and motility. The hemolysin-defective mutants, P10A-C11, P10A-H12, and P79-B5, had inserts in genes involved in RNA turnover (RNase II and 5'-pentaphospho-5'-adenosine pyrophosphohydrolase) and showed reduced virulence and production of extracellular factors. These data support the role of RNA turnover in insect pathogenesis and other physiological functions.

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The importance of proteins of the RNase II/RNB-family in pathogenic bacteria

Cited by 29 publications

References 41 publications

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential

Identification and characterization of Photorhabdus temperata mutants altered in hemolysis and virulence

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