Religious orientation is an attitude toward religion or religious practices or an integrated set of attitudes and beliefs. As religion emphasizes moral codes designed to instil values such as helping, caring, emotional support and empathy in humans so, the study was designed to investigate the relationship between religious orientation and pro-social behavior of young female students. The study also examines the predicting role of religious orientation in the development of prosocial behavior in young female students. The non-probability purposive sampling technique has been used with correlational research design in order to collect data. Analysis was conducted on SPSS by using sample of N=150 young female students with age ranged 20-26 years (M=23.50, SD=3.43). The results showed religious orientation had significant positive association with pro-social behavior (r=.40, p>.00) in young female students. Meanwhile, the findings revealed that religious orientation was a significant positive predictor of prosocial behavior in young female students. The study implies that such types of religious orientation will develop more awareness in youth of the present era about the development of prosocial behaviors like charity and help to the needy.