The Importance of Some New Clinical Indices in the Evaluation of Childhood Obesity

Donma, Mustafa Metın; Donma, Orkide

doi:10.20319/lijhls.2017.33.1628

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…The increase in the prevalence of obesity has become an important health issue throughout the lives of children and adolescents as well as adults [9]. Childhood obesity is the cause of adult obesity and is an important problem, especially because of its relationship with morbid obesity, metabolic syndrome, diabetes mellitus, cancer, and cardiovascular diseases [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Determination of biotin interference in pediatric obesity related ELISA research kits

Kahraman,

DONMA,

Donma

et al. 2024

Preprint

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Background Although high-dose biotin interference is now considered in automated immunoassays, it has not yet been detected in manually studied research kits, especially with enzyme-linked immunosorbent assay (ELISA), and the possibility of biotin interference in these kits has not been demonstrated. The aims of our study were to determine the effects of biotin interference on various parameters, including leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, peroxisome proliferator activated receptor gamma (PPAR γ), nod-like receptor pyrin domain-containing 3 (NLRP3) and interleukin-18 (IL-18), which contribute to energy homeostasis in healthy and obese children. Methods Serum pools were prepared from healthy and obese individuals, and biotin concentrations in samples containing different amounts of biotin were measured via sandwich and competitive ELISA methods. In addition, possible biotin interactions were investigated by determining the concentrations of all the study parameters in serum pools containing different amounts of biotin. Results More consistent results were obtained with biotin-competitive, ghrelin-competitive, KISS1-competitive, GHSR, leptin and LEPR ELISA kits. Unexpectedly, high levels were detected in the biotin sandwich ELISA kit, indicating that biotin interference may also occur in manually run research kits. Conclusions Biotin exhibited an interference effect even in well-functioning, qualified kits, and this negative effect was less common in competitive kits. Biotin interference was closely associated with the quality of the research kit, the parameters studied and the presence of high biotin concentrations in the blood.

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