The importance of the glycosylation of antimicrobial peptides: natural and synthetic approaches

Bednarska, Natalia G.; Wren, Brendan W.; Willcocks, Sam

doi:10.1016/j.drudis.2017.02.001

Cited by 81 publications

(57 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Posttranslational modifications also play a major role in the final three-dimensional structure and bioactivity of AMPs but, unfortunately, cannot be predicted with current in silico tools (Agrawal et al, 2018). The most common posttranslational modifications in natural AFPs involve glycosylation or the addition of carbohydrates, normally observed in asparagine, or serine/threonine residues (Guo et al, 2012;Bednarska et al, 2017). The latter has been shown to improve antifungal activity in some cases.…”

Section: Natural Peptidesmentioning

confidence: 99%

Antifungal Peptides as Therapeutic Agents

Ullivarri

Arbulu

García-Gutiérrez

et al. 2020

Front. Cell. Infect. Microbiol.

174

132

View full text Add to dashboard Cite

Fungi have been used since ancient times in food and beverage-making processes and, more recently, have been harnessed for the production of antibiotics and in processes of relevance to the bioeconomy. Moreover, they are starting to gain attention as a key component of the human microbiome. However, fungi are also responsible for human infections. The incidence of community-acquired and nosocomial fungal infections has increased considerably in recent decades. Antibiotic resistance development, the increasing number of immunodeficiency-and/or immunosuppression-related diseases and limited therapeutic options available are triggering the search for novel alternatives. These new antifungals should be less toxic for the host, with targeted or broader antimicrobial spectra (for diseases of known and unknown etiology, respectively) and modes of actions that limit the potential for the emergence of resistance among pathogenic fungi. Given these criteria, antimicrobial peptides with antifungal properties, i.e., antifungal peptides (AFPs), have emerged as powerful candidates due to their efficacy and high selectivity. In this review, we provide an overview of the bioactivity and classification of AFPs (natural and synthetic) as well as their mode of action and advantages over current antifungal drugs. Additionally, natural, heterologous and synthetic production of AFPs with a view to greater levels of exploitation is discussed. Finally, we evaluate the current and potential applications of these peptides, along with the future challenges relating to antifungal treatments.

show abstract

Section: Natural Peptidesmentioning

confidence: 99%

Antifungal Peptides as Therapeutic Agents

Ullivarri

Arbulu

García-Gutiérrez

et al. 2020

Front. Cell. Infect. Microbiol.

174

132

View full text Add to dashboard Cite

show abstract

“…Glycosylation is one of the most prevalent post-translational modifications of proteins, with a defining impact on their structures and functions. The glycosylation of AMPs can influence their antimicrobial activity, and their ability to affect host immunity, target specificity, and biological stability [36][37][38][39]. The favorable impact of glycosylation on pharmacokinetic properties of the native peptides leads to an increase in their oral absorption and bioavailability [37].…”

Section: Glycosylation Screeningmentioning

confidence: 99%

Antimicrobial Activities of Different Fractions from Mucus of the Garden Snail Cornu aspersum

et al. 2020

View full text Add to dashboard Cite

Natural products have long played a major role in medicine and science. The garden snail Cornu aspersum is a rich source of biologically active natural substances that might be an important source for new drugs to treat human disease. Based on our previous studies, nine fractions containing compounds with Mw <3 kDa; <10 kDa; <20 kDa; >20 kDa; >30 kDa>50 kDa and between 3 and 5 kDa; 5 and 10 kDa; and 10 and 30 kDa were purified from the mucus of C. aspersum and analyzed by tandem mass spectrometry (MALDI-TOF/TOF). Seventeen novel peptides with potential antibacterial activity were identified by de novo MS/MS sequencing using tandem mass spectrometry. The different fractions were tested for antibacterial activity against Gram─ (Pseudomonas aureofaciens and Escherichia coli) and Gram+ (Brevibacillus laterosporus) bacterial strains as well the anaerobic bacterium Clostridium perfringens. These results revealed that the peptide fractions exhibit a predominant antibacterial activity against B. laterosporus; the fraction with Mw 10–30 kDa against E. coli; another peptide fraction <20 kDa against P. aureofaciens; and the protein fraction >20 kDa against the bacterial strain C. perfringens. The discovery of new antimicrobial peptides (AMPs) from natural sources is of great importance for public health due to the AMPs’ effective antimicrobial activities and low resistance rates.

show abstract

“…The affinity of AMP to particular cell surface‐associated glycoproteins explains the selectivity toward cancerous cells. Sapecin, cationic AMP containing KLK motifs, has been shown to interact with negatively charged sialic acid residues on host cell membrane (Bednarska, Wren, & Willcocks, ). Magainin‐2 and its analogues showed rapid and selective cytotoxic activity (12 µg/mL) against hematopoietic and solid tumor cells (Deslouches & Di, ).…”

Section: Antimicrobial Activity and Beyondmentioning

confidence: 99%

Recent insights into structure-function relationships of antimicrobial peptides

2018

View full text Add to dashboard Cite

The application of antimicrobial peptides (AMPs) in food preservation presents a promising alternative and offers many benefits, such as reducing the use of chemical preservatives, reducing food losses due to spoilage, and development of health-promoting food supplements. The biological activity of AMPs largely dependent on several physicochemical features including charge, the degree of helicity, hydrophobicity, and sequence. The present review provides an overview of the structural classification of AMPs emphasizing the importance of their structural features for biological activity, followed by the description of some antimicrobial mechanism of action. Despite the several hurdles that must be overcome for the exploitation of food-derived AMPs in drug discovery and food systems, the developments discussed in this review offer a taste of future trends in food and pharmaceutical applications of these intriguing molecules.

show abstract

The importance of the glycosylation of antimicrobial peptides: natural and synthetic approaches

Cited by 81 publications

References 92 publications

Antifungal Peptides as Therapeutic Agents

Antifungal Peptides as Therapeutic Agents

Antimicrobial Activities of Different Fractions from Mucus of the Garden Snail Cornu aspersum

Recent insights into structure-function relationships of antimicrobial peptides

Contact Info

Product

Resources

About