The importance of the PapR7 C-terminus and amide protons in mediating quorum sensing in Bacillus cereus

Gorgan, Michael; Vanunu Ofri, Shahar; Engler, Emilee R.; Yehuda, Avishag; Hutnick, Elizabeth; Hayouka, Zvi; Bertucci, Michael A.

doi:10.1016/j.resmic.2023.104139

Cited by 3 publications

(1 citation statement)

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“…The flagella of B. cereus aid in movement to suitable locations and the formation of B. cereus biofilm is intricately connected to this locomotion process [16]. It can modulate gene expression through quorum sensing (QS), exercising control over biofilm formation, virulence factors, and spore production [17].…”

Section: Introductionmentioning

confidence: 99%

An Investigation of Quorum Sensing Inhibitors against Bacillus cereus in The Endophytic Fungus Pithomyces sacchari of the Laurencia sp.

Xiang,

Xu,

Yin

et al. 2024

Marine Drugs

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Bacillus cereus, a common food-borne pathogen, forms biofilms and generates virulence factors through a quorum sensing (QS) mechanism. In this study, six compounds (dankasterone A, demethylincisterol A3, zinnimidine, cyclo-(L-Val-L-Pro), cyclo-(L-Ile-L-Pro), and cyclo-(L-Leu-L-Pro)) were isolated from the endophytic fungus Pithomyces sacchari of the Laurencia sp. in the South China Sea. Among them, demethylincisterol A3, a sterol derivative, exhibited strong QS inhibitory activity against B. cereus. The QS inhibitory activity of demethylincisterol A3 was evaluated through experiments. The minimum inhibitory concentration (MIC) of demethylincisterol A3 against B. cereus was 6.25 μg/mL. At sub-MIC concentrations, it significantly decreased biofilm formation, hindered mobility, and diminished the production of protease and hemolysin activity. Moreover, RT-qPCR results demonstrated that demethylincisterol A3 markedly inhibited the expression of QS-related genes (plcR and papR) in B. cereus. The exposure to demethylincisterol A3 resulted in the downregulation of genes (comER, tasA, rpoN, sinR, codY, nheA, hblD, and cytK) associated with biofilm formation, mobility, and virulence factors. Hence, demethylincisterol A3 is a potentially effective compound in the pipeline of innovative antimicrobial therapies.

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Section: Introductionmentioning

confidence: 99%