The importance of understanding the regulation of bacterial metabolism

Moreno, Renata; Rojo, Fernando

doi:10.1111/1462-2920.16123

Cited by 10 publications

(5 citation statements)

References 34 publications

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“…The colonization ability of microorganisms depends on the metabolic strategies that are ligated to the environmental factors that promote genetic expression regulation so as to synthetize biomolecules with diverse functions during competition in an ecological niche with a community structure [58]. Thus, for instance, the competition for nutrients could promote a change in growth rate and could generate metabolites with certain effects on their competitors.…”

Section: Discussionmentioning

confidence: 99%

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity

Hernández Flores,

Martínez,

Ramos López

et al. 2023

Microorganisms

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The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity test revealed nonpathogenic traits confirmed by the absence of specific virulence-related genes. Interestingly, when fungal inhibitory essays were carried out, the bacterial synthesis of volatile organic compounds (VOCs) with antifungal activity showed that Sclerotinia sp. and Rhizoctonia solani were inhibited by 87.45% and 77.24%, respectively. Meanwhile, Sclerotium rolfsii, Alternaria alternata, and Colletotrichum gloeosporioides demonstrated a mean radial growth inhibition of 52.79%, 40.82%, and 55.40%, respectively. The lowest inhibition was by Fusarium oxysporum, with 10.64%. The VOCs’ characterization by headspace solid–phase microextraction combined with gas chromatography–mass spectrometry (HS-SPME-GC–MS) revealed 65 potential compounds. Some of the compounds identified with high relative abundance were ketones (22.47%), represented by 2-butanone, 3-hydroxy (13.52%), and alcohols (23.5%), represented by ethanol (5.56%) and 1-butanol-3-methyl (4.83%). Our findings revealed, for the first time, that K. cowanii Cp1 associated with C. pubescens seeds possesses potential traits indicating that it could serve as an effective biocontrol.

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Section: Discussionmentioning

confidence: 99%

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity

Hernández Flores,

Martínez,

Ramos López

et al. 2023

Microorganisms

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“…Although strategies such as optimizing growth conditions can help to improve the desired performance, it is often necessary to uncouple the expression of genes of interest from the global regulatory networks that optimize metabolism. This requires knowledge of how bacteria control their metabolic networks and the molecular intricacies of the mechanisms involved (reviewed in Moreno & Rojo, 2023 ). While some aspects of these regulatory processes are reasonably well understood, others are not, including the signals that elicit these responses.…”

Section: Introductionmentioning

confidence: 99%

What are the signals that control catabolite repression in Pseudomonas?

Moreno,

Rojo

2024

Microbial Biotechnology

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Metabolically versatile bacteria exhibit a global regulatory response known as carbon catabolite repression (CCR), which prioritizes some carbon sources over others when all are present in sufficient amounts. This optimizes growth by distributing metabolite fluxes, but can restrict yields in biotechnological applications. The molecular mechanisms and preferred substrates for CCR vary between bacterial groups. Escherichia coli prioritizes glucose whereas Pseudomonas sp. prefer certain organic acids or amino acids. A significant issue in understanding (and potentially bypassing) CCR is the lack of information about the signals that trigger this regulatory response. In E. coli, several key compounds act as flux sensors, governing the flow of metabolites through catabolic pathways and preventing imbalances. These flux sensors can also modulate the CCR response. It has been suggested that the order of substrate preference is determined by carbon uptake flux rather than substrate identity. For Pseudomonas, much less information is available, as the signals that induce CCR are poorly understood. This article briefly discusses the available evidence on the signals that trigger CCR and the questions that remain to be answered in Pseudomonas.

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“…Many bacteria can use these pollutants as sources of carbon and energy (Diaz et al., 2013 ; Fuchs et al., 2011 ); however, degradation is slow, in part because many of these molecules are toxic and poorly soluble in water and also because they are often non‐preferred substrates and bacteria tend to use other available carbon sources that are more favourable for growth. Pseudomonas putida can also be used as a biocatalyst to transform aromatic compounds into valuable chemicals (Kohlstedt et al., 2018 ; Nikel & de Lorenzo, 2018 ; Weimer et al., 2020 ), but the presence of preferred carbon sources may compromise the viability of the bioconversion process (Lu et al., 2021 ; Moreno & Rojo, 2023 ; Vinuselvi et al., 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…The prioritization of a particular set of carbon sources over others is a complex phenomenon that helps bacteria to optimize metabolic fluxes, preventing the accumulation of by‐products (Moreno & Rojo, 2023 ). It also facilitates the consumption of compounds that are not used by other microorganisms in a given environment, thereby reducing competition (Park et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic compounds

Moreno,

Yuste,

Morales

et al. 2024

Microbial Biotechnology

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Pyruvate dehydrogenase (PDH) catalyses the irreversible decarboxylation of pyruvate to acetyl‐CoA, which feeds the tricarboxylic acid cycle. We investigated how the loss of PDH affects metabolism in Pseudomonas putida. PDH inactivation resulted in a strain unable to utilize compounds whose assimilation converges at pyruvate, including sugars and several amino acids, whereas compounds that generate acetyl‐CoA supported growth. PDH inactivation also resulted in the loss of carbon catabolite repression (CCR), which inhibits the assimilation of non‐preferred compounds in the presence of other preferred compounds. Pseudomonas putida can degrade many aromatic compounds, most of which produce acetyl‐CoA, making it useful for biotransformation and bioremediation. However, the genes involved in these metabolic pathways are often inhibited by CCR when glucose or amino acids are also present. Our results demonstrate that the PDH‐null strain can efficiently degrade aromatic compounds even in the presence of other preferred substrates, which the wild‐type strain does inefficiently, or not at all. As the loss of PDH limits the assimilation of many sugars and amino acids and relieves the CCR, the PDH‐null strain could be useful in biotransformation or bioremediation processes that require growth with mixtures of preferred substrates and aromatic compounds.

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The importance of understanding the regulation of bacterial metabolism

Cited by 10 publications

References 34 publications

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity

What are the signals that control catabolite repression in Pseudomonas?

Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic compounds

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