The importance of vitellogenin receptors in the oviposition of the pond wolf spider, Pardosa pseudoannulata

Abstract

Vitellogenin receptor (VgR) is crucial for vitellogenin (Vg) uptake by oocytes. VgR is less known in Arachnida, especially in spiders. Different from only one VgR in an arthropod species, two VgRs, VgR‐1 and VgR‐2, were found in the pond wolf spider, Pardosa pseudoannulata. Both VgRs had the typical domains of the low‐density lipoprotein receptor family except for the absence of the ligand‐binding domain 1 in VgR‐2. Spatiotemporal expression profiles showed that two VgR genes were consistently highly expressed in females and their ovaries, but VgR‐1 was 48‐fold that of VgR‐2 in ovaries. The transcriptional level of VgR‐1 was significantly downregulated by RNAi, but it did not work for VgR‐2 although several trials were performed. Vg‐1 and Vg‐2 might be the ligands of VgR‐1 because their expressions were also decreased in the dsVgR‐1‐treated females. Silencing VgR‐1 prolonged the pre‐oviposition period by 56 h. The expression of VgRs and Vgs were upregulated by juvenile hormones (JHs), which suggested that JHs were the essential factors to vitellogenesis in the spider. The present study revealed the importance of VgR‐1 in the spider oviposition, which will improve the understanding on VgR physiological functions in spiders.

Population dynamics and molecular adaption of Tetranychus cinnabarinus to long‐term thermal stress

“…Vitellogenin (Vg) is the precursor of Vitellin, which provides necessary nutrients for the egg development of arthropods, and vitellogenin receptor (VgR) is the key to the uptake of Vg by oocytes. 46 At the molecular level, the decrease of expression of TcVg and TcVgR in T. cinnabarinus mites was detected at high temperatures. Juvenile hormone (JH) can promote the phosphorylation of VgR for Vg deposition, 47 but the change of JH was not obvious under high-temperature conditions in this experiment, so the change of VgR may be regulated by other genes.…”

“…This research suggested that downregulation of FOXO under high temperatures might result in shortened lifespan of T. cinnabarinus . Vitellogenin (Vg) is the precursor of Vitellin, which provides necessary nutrients for the egg development of arthropods, and vitellogenin receptor (VgR) is the key to the uptake of Vg by oocytes 46 . At the molecular level, the decrease of expression of TcVg and TcVgR in T. cinnabarinus mites was detected at high temperatures.…”

EcR/USP-1-mediated ecdysteroid signaling regulates wolf spider (<i>Pardosa pseudoannulata</i>) development and reproduction

“…The ecdysteroid 20-hydroxyecdysone can up-regulate vitellogenin in the Parasteatoda tepidariorum spider ( Bednarek et al, 2019 ). However, the non-viable eggs produced in the ecdysteroid signaling-suppressed P. pseudoannulata females may not be associated with vitellogenesis, as the expression levels of vitellogenin and its receptor are not affected by ecdysteroid application ( Yang et al, 2022 ). Thus, further exploration of the mechanisms underlying non-viable egg production is required.…”

Three Heat Shock Protein Genes and Antioxidant Enzymes Protect Pardosa pseudoannulata (Araneae: Lycosidae) from High Temperature Stress

“…As a powerful tool for gene function studies, dsRNA-mediated silencing or inhibition of target genes has been widely used in various arthropods [ 67 ]. There were also studies using RNAi to silence in P. pseudoannulata [ 68 , 69 ]. Therefore, we performed RNAi experiments by injecting dsRNA into P. pseudoannulata to determine the effect of silencing HSP genes on the heat tolerance of P. pseudoannulata .…”