The improved efficient method for introducing macromolecules into cells using HVJ (Sendai virus) liposomes with gangliosides

Kaneda, Yasufumi; Uchida, Tsuyoshi; Kim, Jeman; Ishiura, Masahiro; Okada, Yoshio

doi:10.1016/0014-4827(87)90331-4

Cited by 97 publications

(40 citation statements)

References 37 publications

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“…The HVJ-liposomes can efficiently deliver encapsulated exogenous molecules directly into cells by means of HVJ-mediated membrane fusion. [14][15][16][17] Using the HVJ-liposome complexes, plasmid DNA and/or oligodeoxynucleotides (ODNs) have been efficiently delivered into a variety of organs and tissues without significant toxicity or inflammation. 18,19 The efficacy and consistent in vivo transfection of the HVJ-liposome system has been well demonstrated in multiple model systems.…”

Section: Discussionmentioning

confidence: 99%

Protective immunization against melanoma by gp100 DNA–HVJ-liposome vaccine

et al. 1999

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Section: Discussionmentioning

confidence: 99%

Protective immunization against melanoma by gp100 DNA–HVJ-liposome vaccine

et al. 1999

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“…[10][11][12]24 Briefly, phosphatidylserine, phosphatiin the basal cistern 2 days after 5 × 10 6 RSV-M glioma dylcholone, and cholesterol were mixed in a weight ratio cells were subcutaneously injected into the cisterna of 1:4.8:2. The lipid mixture (10 mg) was deposited on magna.…”

Section: Figure 5 Survival Curves Of Mg Mice Which Were Intrathecallymentioning

confidence: 99%

“…HVJ-liposomes may contain DNA, and over, meningeal dissemination, the invasion of glioma using this system foreign genes can be transferred very cells into the cerebrospinal fluid, is observed in 15% of efficiently ( Figure 1) and expressed ectopically in dividglioma patients at their terminal stages and may also play ing or nondividing mammalian cells in vivo. [10][11][12][13] To date, a role in the poor prognosis of patients. 2 there have been few applications of HVJ-liposomes to We have used the meningeal gliomatosis (MG) mouse tumor therapy.…”

mentioning

confidence: 99%

Gene delivery by HVJ-liposome in the experimental gene therapy of murine glioma

et al. 1997

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We investigated the delivery of foreign genes into mouse (MG) mouse model system. Highly efficient delivery was glioma cells in vivo using hemagglutinating virus of Japan observed in disseminated glioma cells, and 80% of MG (HVJ)-liposomes, which are coated by Sendai virus envelmice expressing the HSVtk gene were cured by treatment ope protein. HVJ-liposomes, containing lacZ gene or herwith ganciclovir. These results suggest that this novel gene pes simplex virus thymidine kinase (HSVtk) gene-bearing delivery system may be applicable for the in vivo gene therplasmid DNA were applied in the meningeal gliomatosis apy of human malignant glioma.Keywords: malignant glioma; gene transfer; HVJ-liposome; meningeal dissemination; MG mouseIt has been reported that intravenous administration of Introduction liposomes containing the rat insulin I gene resulted in Malignant glioma, especially glioblastoma, is one of the transient expression of rat insulin. 7 However, the most intractable tumors. Patients with glioblastoma have efficiency of liposomal fusion was low. The fusion a median survival of 9 months, despite intensive therapy efficiency was subsequently improved by using spike which may include surgery, irradiation, chemotherapy, proteins 8 or virus particles of HVJ. 9 HVJ-liposomes, and immunotherapy. 1 Poor prognosis is mainly due to which are coated by HVJ (Sendai virus) membranes, are invasion of glioma cells into the brain parenchyma. Morehighly fusigenic. HVJ-liposomes may contain DNA, and over, meningeal dissemination, the invasion of glioma using this system foreign genes can be transferred very cells into the cerebrospinal fluid, is observed in 15% of efficiently ( Figure 1) and expressed ectopically in dividglioma patients at their terminal stages and may also play ing or nondividing mammalian cells in vivo. [10][11][12][13] To date, a role in the poor prognosis of patients. 2 there have been few applications of HVJ-liposomes to We have used the meningeal gliomatosis (MG) mouse tumor therapy. In this study, HVJ-liposomes which conas a model system for patients with glioma. This model tain the lacZ gene or the HSVtk gene-bearing proviral system has been resistant to therapeutic intervention.DNA are applied in the MG mouse model, and we invesHowever, if 25% of stereotactically inoculated intratigate the efficacy of this delivery system for malignant cranial glioma cells are transduced with the herpes simglioma. plex virus type 1 thymidine kinase (HSVtk) gene, all mice can be cured by ganciclovir treatment. 3 Several methods exist for delivering foreign genes into somatic cells. Among them, retroviral vectors have been clinically one of the most frequently used. Because of low infectious titers, however, retroviruses have been used mainly for ex vivo gene delivery to hematopoietic stem cells, 4 or retrovirus-producing cells have been implanted in vivo as a source of continuous production. 5,6 Survival in the MG model could be prolonged by intrathecal administration of HSVtk-producing retrovirus packaging cell...

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“…In the present study, we evaluated the effectiveness of gene therapy against peritoneal metastases, using repeated intraperitoneal transductions of a soluble flt-1 gene mediated by HVJ-cationic liposome. [27][28][29] …”

Section: Introductionmentioning

confidence: 99%

Soluble Flt-1 gene therapy for peritoneal metastases using HVJ-cationic liposomes

et al. 2000

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Many studies have reported a close association between VEGF and tumor angiogenesis. The aim of the present study was to evaluate the effectiveness of gene therapy against cancer, including peritoneal metastasis, using a cDNA encoding a soluble type of Flt-1, one of the VEGF receptors. In a peritoneal metastasis model of MKN45 human gastric cancer cells, mice repetitively treated with intraperitoneal injections of HVJ-Fex, a type of HVJ-cationic liposome encapsulating a plasmid expressing soluble mFlt-1, exhibited smaller disseminated foci with fewer microvessels, thus resulting in a significantly longer survival period than the control mice. In

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The improved efficient method for introducing macromolecules into cells using HVJ (Sendai virus) liposomes with gangliosides

Cited by 97 publications

References 37 publications

Protective immunization against melanoma by gp100 DNA–HVJ-liposome vaccine

Protective immunization against melanoma by gp100 DNA–HVJ-liposome vaccine

Gene delivery by HVJ-liposome in the experimental gene therapy of murine glioma

Soluble Flt-1 gene therapy for peritoneal metastases using HVJ-cationic liposomes

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