To understand the influence of the initial release conditions on the separation characteristics of the store and improve it under high Mach number (Ma = 4) flight conditions, the overset grid method and the Realizable k − ε turbulence model coupled with an equation with six degrees of freedom are used to simulate the store released from the internal bay. The motion trajectory and the attitude angle of the store separation under the conditions of different centroid, velocity, height and control measures are given by the calculated result. Through analysis, the position of the centroid will affect the separation of the store, which needs to be considered in the design. Increasing the launching height is conducive to the separation of the store. If the store has an initial velocity, it can leave the internal bay more quickly and reduce the probability of collision with the wall. Cylindrical rod and slanted aft wall control measures can improve the attitude of the store and make the store fall more smoothly.