The Improvement Effects of Gordonia bronchialis on Male Fertility of Rats with Diabetes Mellitus Induced by Streptozotocin

Abstract

Background: This study evaluated the possible protective effects of Gordonia bronchialis (Gb) on oxidative stress and some subsequent alterations on testis from rats undergoing an experimentally induced type 1 diabetes. Methods: A total of 40 male rats were randomly divided into four groups of ten. Diabetes was induced by injection of 55 mg/kg streptozotocin in 30 rats. Oral administration of Gb at dose of 105 (low dose) and 107 (high dose) CFU/rat was performed in two groups continuously for 14 days. The third and fourth groups received normal saline as the diabetic and healthy control groups, respectively. The blood and testicular tissue samples were taken on the 14th and 21st days post treatment for biochemical and histopathological evaluations. Results: Significant differences were found in blood glucose level, insulin, IL-6 and TNF-α values together with catalase and superoxide dismutase activities and malondialdehyde level in the diabetic group in comparison with healthy and Gb recipient groups. Moreover, the histopathological lesions observed in the diabetic rats mainly included basement membrane thickening, decreased number of Sertoli cells, and severe reduction of spermatogenesis markedly attenuated in Gb-treated rats. Conclusion: Taken together, it seems that oral administration of Gb could ameliorate testicular damage associated with some related parameters in the diabetic animal model.

Comments on "The improvement Effects of Gordonia bronchialis on Male Fertility of Rats with Diabetes Mellitus Induced by Streptozotocin"

“…They also reported that testicular morphology of diabetic rats significantly was improved on days 14 and 21 after the intervention. 1 It should be noted that these findings might be affected by the quality and reproducibility of experimental design mainly the time of study and sample size used in each of the studied time points. Diabetes is now one of the most challenging public health issues of our time.…”

“…We read with interest a recently published article in Pharmaceutical Sciences by Khordadmehr et al, titled "The improvement effects of Gordonia bronchialis on male fertility of rats with diabetes mellitus induced by Streprozotocin". 1 They found that 14 days administration of Gordonia bronchialis could effectively alleviate blood glucose, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA) and improve insulin, superoxide dismutase (SOD) and catalase (CAT) activities in diabetic rats. They also reported that testicular morphology of diabetic rats significantly was improved on days 14 and 21 after the intervention.…”

“…18 However, in the Khordadmehr et al study none of the parameters related to testicular function such as sperm count and testosterone concentration have been measured. 1 In the histopathological examination, Johnsen's scoring system was used to evaluate the overall morphological appearance of seminiferous tubules. In the Khordadmehr et al study, the Johnsen's score for diabetic group was reported around 3.5 and 4.5 on the days 14 and 21, respectively.…”