The improvement of doxorubicin activity on breast cancer cell lines by tangeretin through cell cycle modulation

Meiyanto, Edy; Fitriasari, Aditya; Hermawan, Adam; Junedi, Sendy; Susidarti, Ratna Asmah

doi:10.1007/s13596-011-0016-4

Cited by 27 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This supports our view that this natural compound in combination with Dox, a conventional breast cancer chemotherapeutic agent, is useful to enhance the drug antitumor activity reducing its active concentration and the adverse toxic effect. In particular, we found that the strongly synergistic combination of the two compounds in both cell lines, as evaluated by means of CalcuSyn software, induced an increase of apoptosis and a modulation of cell cycle through a decrease of g0/g1 and S phases and an increase of g2/M phase similarly to Dox, when individually used; in fact, Dox was able to arrest the MCF-7 and t47D breast cancer cell lines at g2/M phase (24). Different studies also have indicated that Dox-induced apoptosis is associated with two distinct apoptotic pathways, i.e., the extrinsic and mitochondrial or intrinsic pathways (19).…”

Section: Discussionmentioning

confidence: 91%

Combining doxorubicin with a phenolic extract from flaxseed oil: Evaluation of the effect on two breast cancer cell lines

Guerriero

Sorice

Capone

et al. 2017

International Journal of Oncology

View full text Add to dashboard Cite

Breast cancer is one of the most frequently diagnosed forms of cancer and different treatments are used to block its progression. However, it still represents a very common cause of death in women. Doxorubicin (Dox) is reported as an effective agent in breast cancer treatment nonetheless it induces many side‑effects. For this reason, many laboratories are engaged in understanding how it is possible to decrease the drug concentration, considering that one of the possible solutions is to use drug synergy, combining it with natural substances. Recently we showed that a phenolic extract from flaxseed (FS) oil, named PEFSO, induced on MCF‑7 cell line an increase of apoptosis with related modification of G0/G1 phase cell cycle, and the activation of signaling and pro‑oxidant pathways. In this study we present data on the combined effect of Dox and PEFSO on two different breast cancer cell lines to define the conditions to use lower doses of this chemotherapeutic agent. We report the data relating to the ability of this mixture to induce cytotoxicity and apoptosis, cell cycle modification, mitochondrial membrane depolarization and activation of extrinsic and/or intrinsic apoptotic pathway.

show abstract

Section: Discussionmentioning

confidence: 91%

Combining doxorubicin with a phenolic extract from flaxseed oil: Evaluation of the effect on two breast cancer cell lines

Guerriero

Sorice

Capone

et al. 2017

International Journal of Oncology

View full text Add to dashboard Cite

show abstract

“…synergistic action of flavonoid, kaempferol or resveratrol along with the Dox. Previously, Docetaxel (anthracycline) loaded lipid nanocapsules have been shown to induce a higher percentage of G2/M arrest in MCF-7 cells than free docetaxel [34].It is also reported earlier that Dox alone or a flavonoid daidzein bring about G2/M arrest in MCF-7 and MDA-MB-453 cells respectively and induce apoptosis [35,36]. Though evidences on the induction of apoptosis by free Dox and also of pure flavonoids have been presented, there are no reports so far on cell cycle arrest by Dox conjugated AuNPs.…”

Section: Morphological Assessmentmentioning

confidence: 89%

Biocompatibility, uptake and anti-cancer activity of doxorubicin loaded gold nanoparticles in MCF-7 breast cancer cell line

Rajaram¹

2016

NSTOA

View full text Add to dashboard Cite

dendrimers, carbon nanotubes, iron oxide nanoparticles, gold nanorods and gold nanoparticles [5-11].Of these, gold nanoparticles (AuNPs) hold immense promise as a carrier due to its large surface to volume ratio, inertness, easy functionalization, low toxicity and ample cell penetration properties [12, 13]. Conjugations of Dox to AuNPs through covalent linkages and surface adsorption have been attempted [14]. In these methods, AuNPs prepared using chemicals such as sodium borohydride or citrate have been utilized which has resulted in non-compatibility towards normal cells. For overcoming this problem, AuNPs synthesized using plant extracts and pure phytochemicals are suggested as alternatives. In addition, delivery of Dox using these AuNPs to cancer cells is also being investigated in recent times. For example, AuNPs prepared using Helminthosporum solani have been used for Dox delivery to cervical cancer cells (HeLa) [15].Azadirachta indica extract capped AuNPs have also been reported to deliver Dox to embryonic kidney cells (HEK239) [16]. Since k-AuNPs have been shown to elicit toxicity to MCF-7 cells and preliminary results obtained in our laboratory have indicated that resveratrol (a stillbenoid) conjugated to AuNPs (r-AuNPs)is also able to bring about apoptosis of MCF-7 cells, it is possible that loading of Dox to k-AuNPs and r-AuNPs may enhance the cytotoxicity [17]. Hence, Dox conjugated k-AuNPs and r-AuNPs have been synthesized and characterized and their actions on MCF-7 cells have been investigated.

show abstract

“…P21 expression causes the inhibition of cell cycle in the G1 and S phases caused by the E/CDK2 and A/CDK2 cycling activity inhibitions. The impeding of the cell cycle in the G1 and S phases causes cancer cell apoptosis induction (Meiyanto, 2011). The synergism effect of EDCG+5 FU combination is presumed that EDCG can be inducted and it can improve 5 FU efficacy through cancer cell apoptosis intrinsic path.…”

Section: Discussionmentioning

confidence: 99%

Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

2018

japs

View full text Add to dashboard Cite

Colon cancer is a malignant neoplasm with high incidence and causes the death of more than 30% of the patients. Although there have been efforts to increase the life of the patient using chemotherapy agents, unspecified targets of drugs cause serious side effects and lead to multiple drug resistance (MDR). This is a major problem in cancer therapy in general. Efforts to use substances from plants that have low toxicity give new hope as a co-chemotherapy agent that can increase the efficacy of chemotherapy agents and reduce their toxicity to normal cells. This study aimed to determine the synergistic effects of therapy combination of Calotropis gigantea (EDCG) leaf extract with the chemotherapy drug 5-Fluorouracil (5-FU) and doxorubicin against WiDR colon cancer cells. The analysis results of MTT showed that the combination of EDCG and 5-Fluorouracil gives synergism effect at the dose combination of EDCG+5FU (3 µg/ml + 62.5 nM; 3 µg/ml + 125 nM; 3 µg/ml + 250 nM). While the combination of doxorubicin and EDCG creates antagonistic effects of mild to the strong antagonist, EDCG may enhance the efficacy of 5 Fluorouracil but decrease the efficacy of doxorubicin. Therefore, a combination of EDCG and 5-Fluorouracil may be recommended for further study.

show abstract

The improvement of doxorubicin activity on breast cancer cell lines by tangeretin through cell cycle modulation

Cited by 27 publications

References 17 publications

Combining doxorubicin with a phenolic extract from flaxseed oil: Evaluation of the effect on two breast cancer cell lines

Combining doxorubicin with a phenolic extract from flaxseed oil: Evaluation of the effect on two breast cancer cell lines

Biocompatibility, uptake and anti-cancer activity of doxorubicin loaded gold nanoparticles in MCF-7 breast cancer cell line

Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

Contact Info

Product

Resources

About