The improvement of phycocyanin stability extracted from Spirulina sp using extrusion encapsulation technique

Suzery, Meiny; Hadiyanto, Hadiyanto; Sutanto, Heri; Soetrisnanto, Danny; Majid, Dian; Setyawan, Deny; Azizah, Nur

doi:10.1063/1.4938296

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…The amount of alginate is proportional to the number of polymer chains available to form the wall material, thereby increasing the coating and hardness of the capsule. Additionally, the influence of CPC on encapsulation efficiency should be considered [8,11,[22][23][24]. Regarding the influence of agavins on encapsulation efficiency, an increase in EE was observed in the case of the M2 matrix.…”

Section: Discussionmentioning

confidence: 99%

Controlled Release of Phycocyanin in Simulated Gastrointestinal Conditions Using Alginate-Agavins-Polysaccharide Beads

Londoño-Moreno,

Mundo-Franco,

Franco-Colin

et al. 2023

Foods

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C-phycocyanin (CPC) is an antioxidant protein that, when purified, is photosensitive and can be affected by environmental and gastrointestinal conditions. This can impact its biological activity, requiring an increase in the effective amount to achieve a therapeutic effect. Therefore, the aim of this study was to develop a microencapsulate of a complex matrix, as a strategy to protect and establish a matrix for the controlled release of CPC based on polysaccharides such as agavins (AGV) using ionic gelation. Four matrices were formulated: M1 (alginate: ALG), M2 (ALG and AGV), M3 (ALG, AGV, and κ-carrageenan: CGN), and M4 (ALG, AGV, CGN, and carboxymethylcellulose: CMC) with increasing concentrations of CPC. The retention and diffusion capacities of C-phycocyanin provided by each matrix were evaluated, as well as their stability under simulated gastrointestinal conditions. The results showed that the encapsulation efficiency of the matrix-type encapsulates with complex composites increased as more components were added to the mixtures. CMC increased the retention due to the hydrophobicity that it provides by being in the polysaccharide matrix; CGN enabled the controlled diffusive release; and AGV provided protection of the CPC beads under simulated gastrointestinal conditions. Therefore, matrix M4 exhibited an encapsulation efficiency for CPC of 98% and a bioaccessibility of 10.65 ± 0.65% after the passage of encapsulates through in vitro digestion.

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Section: Discussionmentioning

confidence: 99%

Controlled Release of Phycocyanin in Simulated Gastrointestinal Conditions Using Alginate-Agavins-Polysaccharide Beads

Londoño-Moreno,

Mundo-Franco,

Franco-Colin

et al. 2023

Foods

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“…Therefore, significant advances have been developed in the modern extraction methods. One of them is ultrasound-assisted extraction (UAE), in which this advance is aimed to the better extraction output (Azwanida, 2015;Sutanto et al, 2015). The purpose of this study was to compare two extraction methods, maceration and ultrasound-assisted extraction (UAE), for obtaining optimum antioxidant.…”

Section: Referencesmentioning

confidence: 99%

Biotechnology for Sustainable Development to Meet Global Challenges

2018

Int J Pharma. Bio. Sci.

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Antimicrobial resistance (AMR) in food-borne pathogens especially Escherichia coli, is of a serious concern in recent years. Extended beta-lactamase resistant (ESBL) resistant E.coli species have recently emerged and have been the source of concern in both human and animal health. This study was conducted to detect the presence of ESBL resistant E.coli in raw chicken meat and bean sprouts in Kota Bharu, Kelantan by using Kirby-Bauer methods for antibiotic sensitivity test (AST) and molecular detection of the ESBL resistance encoding genes by using polymerase chain reaction (PCR). A total of 100 samples comprised of 50 raw chicken meat and 50 bean sprouts were collected and processed microbiologically. Out of 100 food samples that were collected and processed through routine microbiological isolation, 31% (31/100) of E. coli were identified phenotypically. However, based on PCR results, 93.5% (29/31) of the phenotypically detected isolates were confirmed as E.coli. Raw chicken meat yielded 46% (23/50) E.coli isolates while 12% (6/50) E. coli was isolated from bean sprouts samples. Based on the results of AST, raw chicken meat showed highest percentage of antimicrobial resistance, 95.7% (22/23) against Amoxycillin/Clavulanic Acid, followed by Enrofloxacin 60.9% (14/23), Colistin 39.1% (9/23) and Gentamicin 30.4% (7/23). Whereas for bean sprouts, highest percentage of antimicrobial resistance was observed towards Amoxycillin/Clavulanic Acid with the percentage of 33.3% (2/6), followed by Colistin 16.7% (1/6). ESBL gene resistance detection against family of bla TEM and bla CTX genes showed that 62.1% (18/29) of E.coli isolates from both food samples were positive for bla TEM gene and all the isolates were negative for blaCTX. Out of 18 samples positive resistance towards bla TEM gene, 94.4% (17/18) were from raw chicken meat and 5.6% (1/18) were from bean sprouts. In conclusion, ESBL resistant E.coli was detected in the food samples collected from local markets. These findings imply that ESBL resistant E.coli can easily contaminate raw food items such as chickens and vegetables and may pose public health risks. Hence, rational usage of antibiotics and strict practicing of hygienic handling of food items, especially chicken meat and vegetables are recommended.

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“…Studies have been conducted on the incorporation of C-PC into polymer carriers (hydrogel [8], microcapsules [13], liposomes [14]), the kinetics of C-PC release from the polymer matrix, and the subsequent wound-healing effect in experiments in vitro and in vivo. Thus, C-PC is a promising component of wound-healing materials, and its inclusion in the polymer matrix allows one to control the rate of its release, prolonging its action.…”

Section: Introductionmentioning

confidence: 99%

Chitosan Sponges for Efficient Accumulation and Controlled Release of C-Phycocyanin

Sergeeva,

Zakharevich,

Sukhinov

et al. 2023

BioTech

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The paper proposed a new porous material for wound healing based on chitosan and C-phycocyanin (C-PC). In this work, C-PC was extracted from the cyanobacteria Arthrospira platensis biomass and purified through ammonium sulfate precipitation. The obtained C-PC with a purity index (PI) of 3.36 ± 0.24 was loaded into a chitosan sponge from aqueous solutions of various concentrations (250, 500, and 1000 mg/L). According to the FTIR study, chitosan did not form new bonds with C-PC, but acted as a carrier. The encapsulation efficiency value exceeded 90%, and the maximum loading capacity was 172.67 ± 0.47 mg/g. The release of C-PC from the polymer matrix into the saline medium was estimated, and it was found 50% of C-PC was released in the first hour and the maximum concentration was reached in 5–7 h after the sponge immersion. The PI of the released C-PC was 3.79 and 4.43 depending on the concentration of the initial solution.

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The improvement of phycocyanin stability extracted from Spirulina sp using extrusion encapsulation technique

Cited by 8 publications

References 8 publications

Controlled Release of Phycocyanin in Simulated Gastrointestinal Conditions Using Alginate-Agavins-Polysaccharide Beads

Controlled Release of Phycocyanin in Simulated Gastrointestinal Conditions Using Alginate-Agavins-Polysaccharide Beads

Biotechnology for Sustainable Development to Meet Global Challenges

Chitosan Sponges for Efficient Accumulation and Controlled Release of C-Phycocyanin

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