The in-capillary- 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)-sweeping micellar electrokinetic chromatography - Diode array detector method for screening and quantifying trace natural antioxidants from Schisandra chinensis

Ma, Huifen; Liu, Tao; Li, Jin; Ding, Mingya; Gao, Xiumei; Chang, Yan‐xu

doi:10.1016/j.chroma.2019.01.050

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…EOF affected by pH is an important parameter to change the separation efficiency and migration time of compounds in CE [15]. When pH is less than 4.0, the hydroxyl dissociation of capillary inner wall would be inhibited, which might lead to a suppressed EOF, and the adsorption on the inner wall may also be reduced [25].…”

Section: Resultsmentioning

confidence: 99%

“…Antioxidant evaluation of ABTS + was determined through the rate of ABTS oxidation by laccase. More and more attention has been attracted, and researchers have developed various online and offline methods that use ABTS + as oxidation‐free radicals including UV [11], HPLC [12], ultra performance LC (UPLC) [13], UPLC/MS [14], and CE [15]. The online mode made reaction, screening, and detection integrated, so the screening and evaluation of active ingredients could be simultaneously realized.…”

Section: Introductionmentioning

confidence: 99%

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A novel reverse migration micellar electrokinetic chromatography method for in‐capillary screening and quantifying of antioxidant components in Sanyetangzhiqing using 2,2′‐Azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) as oxidation‐free radical

et al. 2022

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A novel method of reverse migration micellar electrokinetic chromatography (RM-MEKC) using 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) as oxidation-free radical was developed to screen the major antioxidants from Sanyetangzhiqing (SYTZQ), which is a new patent drug for diabetes. For simultaneous detection and separation of 2,2′-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS + ) and chemical components of SYTZQ, the key factors were optimized and the method was validated under the optimal conditions. All the relative standard deviations of recovery, precision, and stability were below 6.6%. Based on these, the RM-MEKC method has been successfully used to screen the main antioxidants from SYTZQ tablets. Five compounds, including rosmarinic acid, salvianolic acid B, lithospermic acid, hyperoside, and isoquercetin, were separated and identified as the major antioxidants. There was a linear relationship with correlation coefficient of 0.923 between the total amount of major antioxidants and total antioxidative activity of SYTZQ. Consequently, these five ingredients could be selected as combinatorial markers for quality control of SYTZQ, and the established method was concluded to be a simple, reliable, and powerful tool to screen and quantify active compounds for quality evaluation of SYTZQ.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel reverse migration micellar electrokinetic chromatography method for in‐capillary screening and quantifying of antioxidant components in Sanyetangzhiqing using 2,2′‐Azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) as oxidation‐free radical

et al. 2022

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“…Qualitative and quantitative methods based on MEKC are rapid, efficient, and ecofriendly, and are successfully employed for the routine quality control of herbal drugs and raw plant material (see Table 2). [123] nd-no data, LOQ-limit of quantification, LOD-limit of detection, SDS-sodium dodecyl sulfate, SB-sodium borate, MSPD-micro matrix solid phase dispersion, TAPS-N-[(1S,2S,3R)-2,3-bis(acetyloxy)-1-[(acetyloxy)methyl]heptadecyl]-acetamide, DOC-anionic detergents sodium deoxycholate, ILs-MWNTs-ionic liquids coated multi-walled carbon nanotubes, β-CD-β-cyclodextrin, HP-β-CDhydroxypropyl-β-cyclodextrin, THSG-2,3,5,4 -tetrahydroxystilbene 2-O-β-D-glucoside, SC-sodium cholate.…”

Section: Micellar Electrokinetic Chromatography (Mekc)mentioning

confidence: 99%

Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations

et al. 2021

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The present review summarizes scientific reports from between 2010 and 2019 on the use of capillary electrophoresis to quantify active constituents (i.e., phenolic compounds, coumarins, protoberberines, curcuminoids, iridoid glycosides, alkaloids, triterpene acids) in medicinal plants and herbal formulations. The present literature review is founded on PRISMA guidelines and selection criteria were formulated on the basis of PICOS (Population, Intervention, Comparison, Outcome, Study type). The scrutiny reveals capillary electrophoresis with ultraviolet detection as the most frequently used capillary electromigration technique for the selective separation and quantification of bioactive compounds. For the purpose of improvement of resolution and sensitivity, other detection methods are used (including mass spectrometry), modifiers to the background electrolyte are introduced and different extraction as well as pre-concentration techniques are employed. In conclusion, capillary electrophoresis is a powerful tool and for given applications it is comparable to high performance liquid chromatography. Short time of execution, high efficiency, versatility in separation modes and low consumption of solvents and sample make capillary electrophoresis an attractive and eco-friendly alternative to more expensive methods for the quality control of drugs or raw plant material without any relevant decrease in sensitivity.

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“…49 Gao et al 50 introduced Schisandrin, Schisandrol B, Deoxyschisandrin, Gomisin H and Schisandrin B as the five major lignans in S. Chinensis oils. Ma et al 51 Identified seven compounds including schizandrin, schisandrol B, Schisantherin B, schisantherin A, schisanhenol, deoxyschizandrin and schisandrin B as the main antioxidants in Schisandra chinensis. Chemical structure of Gomisin C (GC) and Gomisin G (GG) is shown in Figure 1.…”

Section: Chemical Constituentsmentioning

confidence: 99%

Schisandra chinensis, Five Flavor Berry, a Traditional Chinese Medicine and a Super-Fruit from North Eastern China

Sun¹,

Shahrajabian²,

Cheng³

2021

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Introduction:The aim of this review was to summarize the most important traditional and modern health benefits of Schisanda berry. Methods: We used PubMed, Science Direct and Google to search for and collect scientific publication for a full evaluation of current evidence in the literature indicating the potential role of Schisanda berry. Results: The traditional Chinese medicine (TCMs) is essential components of alternative medicines. Schisandra berry (Schisandra chinensis (Turcz.) Baill. (Magnoliaceae) or Wu-Wei-Zi, which means the fruit of five tastes is a commonly used herb in traditional Chinese medicine (TCM). The five tastes of Schisandra berry refer to its influence on the five visceral organs in the body. It is one of the oldest medicinal plants used in East of Asia, especially traditional Chinese medicine. S. chinensis is mainly distributed in provinces in northern China including Heilongjiang, Jilin and Liaoning. S. chinensis can treat lung-Qi and kidney-Yin deficiencies, relieve coughs and asthma and stop persistent diarrhea. S. chinensis has been used to treat sleep disorder in traditional Chinese medicine and a tonic for kidney and brain in traditional Chinese medicine. Its extracts may apply as a preservative and as an additive to increase the flavor, taste and nutritional value of food. The five important components of S. chinensis are Schisandrol B, Schisandrin A, γ-Schisandrin, Gomisin N and Schisandrin C. Conclusion:The most important pharmacological effects of Schisandrol B are antiallergy, liver protection and anticarcinogenic effects; Schisandrin A has role in liver protection and anti-carcinogenic effect; γ-Schisandrin has liver protection and anti-carcinogenic effect, Gomisin N has anti-proliferative, pro-apoptis HepG2 cells, anti-hepatitis, anti-HIV, anti-carcinogenic effect; and Schisandrin C has protect liver, anti-HIV, anti-catchinogenic effect and anti-hepatitis. The treatment with natural herbal medicine which are also super-fruit and non-synthetic drug is recommended.

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The in-capillary- 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)-sweeping micellar electrokinetic chromatography - Diode array detector method for screening and quantifying trace natural antioxidants from Schisandra chinensis

Cited by 17 publications

References 33 publications

A novel reverse migration micellar electrokinetic chromatography method for in‐capillary screening and quantifying of antioxidant components in Sanyetangzhiqing using 2,2′‐Azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) as oxidation‐free radical

A novel reverse migration micellar electrokinetic chromatography method for in‐capillary screening and quantifying of antioxidant components in Sanyetangzhiqing using 2,2′‐Azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) as oxidation‐free radical

Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations

Schisandra chinensis, Five Flavor Berry, a Traditional Chinese Medicine and a Super-Fruit from North Eastern China

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