The In Situ Sulfidation of Cu₂O by Endogenous H₂S for Colon Cancer Theranostics

Abstract

Expression of ahigh concentration of H 2 Sisclosely related to the formation of colon cancer tumors.However,only af ew H 2 S-triggered theranostics agents for colon cancer have been reported to date.N ow,at urn-on theranostics agent was developed by utilizing the in situ reaction of Cu 2 Oa nd endogenous H 2 Sa tc olon tumor sites.B ased on in vitro and in vivo experiments,e xcellent photoacoustic imaging and photothermal therapyw ere both confirmed by this in situ reaction-activated colon cancer theranostics method. This work established as imple and efficient strategy for both diagnosis and treatment of colon cancer with an ovel trigger mechanism, whichp rovides an ew route for colon cancer theranostics based on the in situ reactions at the tumor sites.

H 2 S-Activated “One-Key Triple-Lock” Bis-Metal Coordination Network for Visualizing Precise Therapy of Colon Cancer

“…18 Meanwhile, AOAA, an inhibitor of the H 2 S-producing enzyme CBS, was employed to reduce the H 2 S level within cells (Figure 4a). 4 Confocal images of HCT116 cells incubated with Gd/Cu-nanosheets illustrated apparent red fluorescence intensity owing to the activation of Gd/Cu-nanosheets by endogenous H 2 S (Figure 4b). In comparison with that treated with Gd/Cu-nanosheets alone, HCT116 cells incubated with AOAA and Gd/Cu-nanosheets showed much lower fluorescence; meanwhile, HCT116 cells incubated with L-Cys + Gd/Cunanosheets displayed a significant increase in intracellular fluorescence (Figure 4b).…”

“…Meanwhile, 693,900 deaths occurred, making it the fourth most common cause of cancer-related mortality. [1][2][3][4] At present, surgical procedures are routinely used in clinical colon cancer treatment, often combined with radiation therapy or chemotherapy. 5,6 However, such treatment approaches suffer the lack of specificity toward tumors, usually resulting in severe damage to normal body tissues, including generalized toxicity and pain.…”

“…30 Via "turn-on" photoacoustic effect, the in situ sulfidations of Cu 2 O or Au@Cu 2 O nanoparticles by endogenous H 2 S were further employed to visualize colon cancer. 4,31 Dualmodality optical/photoacoustic probes have also been developed for ratiometric imaging of stimulated H 2 S upregulation in mice. 32 However, these imaging technologies still have limitations for whole-body imaging of in vivo tumors owing to poor tissue penetration of the excitation laser for fluorescence or photoacoustic imaging, even for the emerging NIR-II fluorescence.…”

Endogenous H2S-Triggered In Situ Synthesis of NIR-II-Emitting Nanoprobe for In Vivo Intelligently Lighting Up Colorectal Cancer

“…Among adults older than 50 years, colorectal cancer incidence rates had increased by 22% from 2000 to 2013, which was one of the most common malignancies in the world (Siegel et al., 2017, Ricci-Vitiani et al., 2006). Also, some clinical diagnostic methods for colorectal cancer such as colonoscopy, surgical procedures, and carcinoembryonic antigen are well explored (Hassan and Repici, 2017, Zhang et al., 2017a, Zhang et al., 2017b, Xu et al., 2018, An et al., 2018). However, all these traditional methods still suffer from some inevitable drawbacks, including extensive medical experience needed, low sensitivity, and great pain to patients (Hassan and Repici, 2017, Zhang et al., 2017a, Zhang et al., 2017b, Xu et al., 2018, An et al., 2018).…”

Enhanced Plasmon-Induced Resonance Energy Transfer (PIRET)-Mediated Photothermal and Photodynamic Therapy Guided by Photoacoustic and Magnetic Resonance Imaging

“…In the Au@PB@Cu 2 O nanosystem, PB-modified gold nanorods can equip Au@Cu 2 O NPs with PTT effect, while the traditional Au@Cu 2 O NPs were unable to exhibit the property due to their relatively low absorption throughout the NIR region. 38 In addition, we utilized PB to separate gold nanorods and Cu 2 O to take full advantage of plasmonic gold nanorods and consequently improved the photocatalytic activity on the surface of Cu 2 O, generating more 1 O 2 to kill the cancer cells. Furthermore, it has been verified that Prussian blue displays a catalase-like activity to catalyze the decomposition of hydrogen peroxide (H 2 O 2 ) into oxygen (O 2 ) molecules under the neutral pH condition (pH = 7.4).…”