2000
DOI: 10.1016/s0014-5793(00)01528-3
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The in vitro activity of ADAM‐10 is inhibited by TIMP‐1 and TIMP‐3

Abstract: A recombinant soluble form of the catalytic domain of human ADAM-10 was expressed as an Fc fusion protein from myeloma cells. The ADAM-10 was catalytically active, cleaving myelin basic protein and peptides based on the previously described`metallosheddase' cleavage sites of tumour necrosis factor K K, CD40 ligand and amyloid precursor protein. The myelin basic protein degradation assay was used to demonstrate that hydroxamate inhibitors of matrix metalloproteinases (MMPs) were also inhibitors of ADAM-10. The … Show more

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Cited by 370 publications
(279 citation statements)
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“…However, the majority of proteins that undergo regulated shedding appear to be cleaved by TACE [Arribas and Borroto, 2002]. TIMP-3 inhibits both ADAM10 and TACE, whereas TIMP-1 inhibits ADAM10 but not TACE [Amour et al, 1998;Amour et al, 2000]. Both these ADAMs are expressed in HEK cells [Slack et al, 2001], and our results could suggest that collagen-dependent DDR1 shedding is mediated either by TACE alone, which would account for the resistance of the response to TIMP-1, or by both TACE and ADAM10.…”
Section: Discussionmentioning
confidence: 69%
“…However, the majority of proteins that undergo regulated shedding appear to be cleaved by TACE [Arribas and Borroto, 2002]. TIMP-3 inhibits both ADAM10 and TACE, whereas TIMP-1 inhibits ADAM10 but not TACE [Amour et al, 1998;Amour et al, 2000]. Both these ADAMs are expressed in HEK cells [Slack et al, 2001], and our results could suggest that collagen-dependent DDR1 shedding is mediated either by TACE alone, which would account for the resistance of the response to TIMP-1, or by both TACE and ADAM10.…”
Section: Discussionmentioning
confidence: 69%
“…ADAM10 has been shown to be inhibited by tissue inhibitor of metalloproteases (TIMPs) 24 through the activity of their NTR module 24,25 . Because Sfrp1/2 contain an NTR module 8 , we postulated that Sfrp1/2 may normally down-regulate the activity of ADAM10.…”
Section: Adam10 Inhibition Rescues the Sfrp Ko Retinal Phenotypementioning
confidence: 99%
“…The Tissue Inhibitor of Metalloproteinases (TIMPs) demonstrate selectivity in their inhibition of ADAMs and ADAMTSs which contrasts with their MMP-inhibitory features [26][27][28][29]. For example, ADAM-17 is exclusively inhibited by TIMP-3, ADAM-10 is sensitive to TIMP-1 and TIMP-3, but not to TIMP-2 and TIMP-4.…”
Section: Structural Features Of Adams and Adamtssmentioning
confidence: 99%