The in vitro antimicrobial activities of four endodontic sealers

Abstract

Background The purpose of this study was to investigate the antimicrobial activities of four endodontic sealers (GuttaFlow2, AH Plus, ProRoot MTA and RealSeal) against E. feacalis , E.coli and C.albicans . Methods The antimicrobial activities of four endodontic sealers were assessed by both agar diffusion test (ADT) and direct contact test (DCT) in this study. In ADT, the results were reported as the diameter of the growth inhibition zone. Both fresh and 1-day-setting sealers were measured. In DCT, microorganisms in suspension were exposed to the sealers for 10, 30 and 60 min and the survival of microorganisms were determined after exposure at different time points(after mixing, 1 and 7 days). The number of colony-forming unit (CFU) was counted. The results were analyzed with ANOVA and Tukey tests. Results Both ADT and DCT results showed that Guttaflow2 presented no effect against any tested microorganisms. In ADT, fresh RealSeal had the largest inhibition zone against all tested microbes, followed by AH Plus and ProRoot MTA. ProRoot MTA demonstrated inhibition zones against all the three test microbes after setting for 1 day, while the other three sealers showed no inhibition activity. In DCT, fresh AH Plus had the strongest antimicrobial effects against all the three tested microorganisms for every contact times, while its antimicrobial activity diminished significantly with time. Fresh RealSeal and ProRoot MTA also showed strong antimicrobial effect and still showed antimicrobial effect after 1-day-setting. The antibacterial effects of RealSeal against E. faecalis and antifungal effect of ProRoot MTA were observed after 7 days of setting. Conclusions GuttaFlow2 had no antimicrobial activity. Freshly mixed RealSeal and AH Plus demonstrated strong antimicrobial effects. RealSeal showed antimicrobial effects after setting in DCT. ProRoot MTA showed high antimicrobial activity and exhibited anti-inflammation potentials after setting.

The Effectiveness of Laser-Activated Irrigation on the Apical Microleakage Qualities of MTA Repair HP and NeoMTA Plus in Simulated Immature Teeth: A Comparative Study

“…MTA Repair HP and NeoMTA Plus were compared with other three calcium silicate-based cements (Biodentine, ProRoot MTA, MTA Angelus) to better evaluate its antifungal and antibacterial activities against C. albicans, S. aureus, E. faecalis and P. aeruginosa . In accordance with the study of Huang et al, [ 41 ], all five tested materials exhibited strong antifungal activity against C. albicans . All materials showed effective antibacterial activity against S. aureus and P. aeruginosa at 24 h. Although there was no clear indication of inhibitory activity of the materials tested against E. faecalis at 24 h, slight antibacterial activity was detected after 48 h. The ineffective antibacterial activity of all five tested materials against E. faecalis during 24 h may be associated with the resistant phenotype of E. faecalis .…”

Potential Application of Human β-Defensin 4 in Dental Pulp Repair

“…So far, there are many reports about the antibacterial activity of MTA, but the conclusions are different. The reason may be related to the difference of experimental design, detection methods and strains ( Hernandez-Delgadillo et al, 2017 ; Shin et al, 2017 ; Huang et al, 2019 ). Moreover, their restorative effect on dental pulp do not follow the natural process of pulp-dentin complex.…”

Antibacterial and Solubility Analysis of Experimental Phytotherapeutic Paste for Endodontic Treatment of Primary Teeth

“…The antibacterial effect of the pastes was evaluated by the direct contact test [15]. Test specimens (n=5/group) [16,17] were immersed in 2 ml of culture medium containing E. faecalis bacterial inoculum (ATCC 29212) at 1×108 CFU/ml concentration.…”