The in-vitro conservation of valuable genetic resources

Mix-Wagner, Gunda; Schumacher, Heinz Martin

doi:10.1007/978-3-7091-6040-4_8

Cited by 1 publication

(4 citation statements)

References 18 publications

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“…For extending the storage period, it is possible to use reduced temperature, reduced light conditions, and a modification of media, particularly the addition of growth retardants (Fletcher et al 1998, Mix-Wagner 1999. Slow-growth and microtuber regimes are used as medium-term storage option.…”

Section: Potatomentioning

confidence: 99%

“…They were placed on filter paper wetted with MSTo medium (Mix-Wagner 1999). When the plantlets were 10 cm tall, the shoot tips were cut off.…”

Section: Potatomentioning

confidence: 99%

“…We applied a simple cryopreservation method for asparagus, using in-vitro cultured shoot tips, by the "droplet" method originally developed for potato (Schafer-Menuhr et al 1996, Mix-Wagner 1999.…”

Section: Asparagusmentioning

confidence: 99%

“…Apices consisting of the apical bud and two leave primordia were cryopreserved using the droplet method (Mix-Wagner et al 1999). The eight varieties: Astro, Branilo, Branglow, Heidi weiss, (outdoor flower), Elegance weiss, Snowdon (cut flower), Trumpf rot, White Cindy (pot flower) were supplied by Brandkamp (lsselburg, D).…”

Section: Chrysanthemummentioning

confidence: 99%

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Plant Tissue Culture

Laimer¹,

Rücker²

2003

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PrefaceOn February 6 th , 1902 appeared Gottlieb Haberlandts publication on "Culturversuche mit isolierten Pflanzenzellen" by the Imperial Academy of Sciences in Vienna, Austria. This paper contains Haberlandt's vision on the totipotency of plant cells, an idea with worldwide dimensions representing the actual beginning of tissue culture. When Haberlandt started culturing isolated plant cells in artificial nutrient media, he was mainly interested in cell to cell relationships within a complex multicellular organism. Haberlandt had studied the physiology of the plant tissues for a long time, and such profound dedication allowed him to understand the connection between physiology and anatomy. On that basis he has been able to make such a daring vision.In fact, it was only in 1912 that A Carrel succeeded in establishing the first animal cell cultures -which was awarded a Nobel prize -and in 1934 and 1939 respectively, the first plant tissue cultures were achieved by P.A White, R.J. Gautheret and P. Nobecourt. Commercial applications of plant tissue culture followed only in the seventies. We consider this an excellent example for the time frame required from a vision to its realisation and further to its development and broad range application.We take this anniversary also as an opportunity to remind all of us, that for developing ideas time is needed together with right conditions and atmosphere. Universities in the past used to provide, what now we are risking to lose with our hectic life style and short-term planning.This book pays homage to a great Austrian scientist, describing his life and his work and the further development, success and application of his idea. Most students and scientists relate the name Haberlandt with the first lectures of plant tissue culture. But only few are acquainted with the original pieces of work, which apart from their scientific interest are true artistic masterpieces. That is why the first part of the book contains a facsimile of the original paper and its first translation into English by AD. Krikorian and D.L. Berquam in 1969. Furthermore we find it worthwhile to pay some closer attention to the way experiments were planned, carried out and evaluated by Haberlandt in his time.It was also our intention to show the development which the initial idea has made in the past spreading into the various methods and fields of application. The second and third part of the book describe Haberlandt's life and work and historical aspects of the development of plant tissue culture in the beginnings. Who could give a better glance into the early days of plant tissue culture than R.J. Gautheret? It was from his laboratory in Paris that the senior editor brought this technique and knowledge to Vienna and established this field of Preface research here in Austria. The forth part of the book contains an overview of important topics of plant tissue culture representing the most promising areas of application at present time and giving an outlook into the future. The areas range from micropropagati...

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Section: Potatomentioning

confidence: 99%

“…They were placed on filter paper wetted with MSTo medium (Mix-Wagner 1999). When the plantlets were 10 cm tall, the shoot tips were cut off.…”

Section: Potatomentioning

confidence: 99%

Section: Asparagusmentioning

confidence: 99%

Section: Chrysanthemummentioning

confidence: 99%

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