The In-vitro Effect of Indomethacin on Uterine Muscle Contraction Stimulated by Acetone Leaf Extract of Anogeissus Leiocarpus

Chiroma, Mbursa; William, Arastus; Mshelia, Peter; Sandabe, Umar Kyari; Uchendu, C. N.; Chiroma, Ijuptil

doi:10.5455/ijlr.20190205030234

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“…In addition to the numerous medicinal uses of A. leiocarpus in folk medicine, local livestock farmers in North Eastern Nigeria claim that ingestion of leaves of A. leiocarpus induces abortion in small ruminants (Chiroma et al, 2019).This study was therefore designed to examine the contractile effects of acetone leaf extract of this medicinal plant on isolated uterine muscle strips of the rat, and the possible role of calcium in this contractile process as a prelude to its in vivo effects on female reproduction. The research will also shed light on the veracity of its claimed abortifacient effects in small ruminants (sheep and goat) by local livestock farmers in the study area.…”

Section: Introductionmentioning

confidence: 99%

Influence of extracellular calcium on in vitro uterine muscle contractions stimulated by acetone leaf extract of Anogeissus leiocarpa

Chiroma¹,

Madziga²,

Chiroma³

et al. 2021

J. Pharmacognosy Phytother.

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The effect of extracellular calcium on isolated uterine muscle contractions stimulated by acetone leaf extract of Anogeissus leiocarpa was investigated in a rat. About 12 m segment of the uterine muscle strip was mounted initially in a thermostatically regulated organ bath (37°C) containing normal Physiological Salt Solution (PSS; De Jalon) and later inphysiological salt solution devoid of CaCl 2 . The extract contracted the uterine muscle preparation in a concentration-related manner in normal PSS, with 0.53 mg/ml as the lowest active concentration. This contractile response was abolished by the calcium channel blocker, verapamil HCl (2 µg/ml). The extract however, did not evoke any contractile activity on the isolated tissue in a calcium-free media (PSS), even when caffeine (2 mmol) known to release calcium via the calcium induced-calcium release (CICR) mechanism was added to the perfusate. The results demonstrated the requirement for extracellular calcium for the extract mediated contractions and the inability of the extract to access calcium from intracellular storage sites such as the sarcoplasmic reticulum and the mitochondria. Therefore, the influx of Ca 2+ into the cell cytosol is a pre-requisite for the extract-mediated uterine muscle contraction.

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Section: Introductionmentioning

confidence: 99%