The in vitro effect of a lyophilized extract of wine obtained from Jacquez grapes on human chondrocytes

Panico, Annamaria; Cardile, Venera; Avondo, Sergio; Garufi, F.; Gentile, Barbara Cristina; Puglia, Carmelo; Bonina, Francesco; Santagati, Natale Alfredo; Ronsisvalle, Giuseppe

doi:10.1016/j.phymed.2005.06.009

Cited by 33 publications

(24 citation statements)

References 9 publications

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“…While previous studies showed that procyanidins exert a chondroprotective effect in part by suppressing oxidative stress-induced factors [29,30,32], we revealed that procyanidins inhibit VEGF-induced signaling in the articular cartilage of DMM-induced OA mice. VEGF signaling plays a significant role in OA progression, including enhancing expression of pro-inflammatory cytokines and catabolic mediators in chondrocytes [54,70].…”

Section: Discussioncontrasting

confidence: 57%

“…To examine the effect of procyanidins on VEGF signaling, human primary chondrocytes were incubated with 0–100 µg/mL procyanidins [29,32,83] at 37 °C, 5% CO 2 for 1 h. Then the cells were stimulated with 100 ng/mL exogenous human recombinant VEGF (Gemini) for 5 min. The cells were then lysed for Western blot to detect the expression of total VEGFR-2 and the phosphorylation of VEGFR-2.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro studies have shown procyanidins suppress interleukin (IL)-1β-induced increases in reactive oxygen species (ROS) production in human chondrocytes [29]. In animal studies, grape seed extract, rich with procyanidins, reduce ROS activity and expression of matrix metalloproteinases (MMPs) in the articular cartilage of monosodium iodoacetate-induced OA rats [30], and procyanidins extracted from grape seeds ameliorate collagen-induced arthritis (CIA) in mice through regulation of the toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) signaling pathway [31].…”

Section: Introductionmentioning

confidence: 99%

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Procyanidins Mitigate Osteoarthritis Pathogenesis by, at Least in Part, Suppressing Vascular Endothelial Growth Factor Signaling

Wang

Leong

et al. 2016

IJMS

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Procyanidins are a family of plant metabolites that have been suggested to mitigate osteoarthritis pathogenesis in mice. However, the underlying mechanism is largely unknown. This study aimed to determine whether procyanidins mitigate traumatic injury-induced osteoarthritis (OA) disease progression, and whether procyanidins exert a chondroprotective effect by, at least in part, suppressing vascular endothelial growth factor signaling. Procyanidins (extracts from pine bark), orally administered to mice subjected to surgery for destabilization of the medial meniscus, significantly slowed OA disease progression. Real-time polymerase chain reaction revealed that procyanidin treatment reduced expression of vascular endothelial growth factor and effectors in OA pathogenesis that are regulated by vascular endothelial growth factor. Procyanidin-suppressed vascular endothelial growth factor expression was correlated with reduced phosphorylation of vascular endothelial growth factor receptor 2 in human OA primary chondrocytes. Moreover, components of procyanidins, procyanidin B2 and procyanidin B3 exerted effects similar to those of total procyanidins in mitigating the OA-related gene expression profile in the primary culture of human OA chondrocytes in the presence of vascular endothelial growth factor. Together, these findings suggest procyanidins mitigate OA pathogenesis, which is mediated, at least in part, by suppressing vascular endothelial growth factor signaling.

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Section: Discussioncontrasting

confidence: 57%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Procyanidins Mitigate Osteoarthritis Pathogenesis by, at Least in Part, Suppressing Vascular Endothelial Growth Factor Signaling

Wang

Leong

et al. 2016

IJMS

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“…As natural compounds, grape flavonoids and proanthocyanidins can target multiple pathways to overcome chronic inflammation, and thus are more effective compared to synthetic mono-targeted anti-inflammatory drugs [78]. Freeze-dried extract of wine from “Jacquez” grapes ( Vitis aestivalis-cinerea × Vitis vinifera ), which contains mainly flavonoids, anthocyanins, proanthocyanidins and hydroxycinnamic acid derivatives, showed higher anti-inflammatory activity when compared to the commercial non-steroidal anti-inflammatory drug (NSAID) indomethacin [79]. It has also been demonstrated that proanthocyanidins in grape seeds have high anti-inflammatory action, because they scavenge free radicals, prevent lipid peroxidation and inhibit formation of pro-inflammatory cytokines [80].…”

Section: Biological Activities Of Grape Productsmentioning

confidence: 99%

Recent Advances and Uses of Grape Flavonoids as Nutraceuticals

2014

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Grape is one of the oldest fruit crops domesticated by humans. The numerous uses of grape in making wine, beverages, jelly, and other products, has made it one of the most economically important plants worldwide. The complex phytochemistry of the berry is characterized by a wide variety of compounds, most of which have been demonstrated to have therapeutic or health promoting properties. Among them, flavonoids are the most abundant and widely studied, and have enjoyed greater attention among grape researchers in the last century. Recent studies have shown that the beneficial health effects promoted by consumption of grape and grape products are attributed to the unique mix of polyphenolic compounds. As the largest group of grape polyphenols, flavonoids are the main candidates considered to have biological properties, including but not limited to antioxidant, anti-inflammatory, anti-cancer, antimicrobial, antiviral, cardioprotective, neuroprotective, and hepatoprotective activities. Here, we discuss the recent scientific advances supporting the beneficial health qualities of grape and grape-derived products, mechanisms of their biological activity, bioavailability, and their uses as nutraceuticals. The advantages of modern plant cell based biotechnology as an alternative method for production of grape nutraceuticals and improvement of their health qualities are also discussed.

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“…Phenolic compounds in grapes, especially in grape seeds, have showed significant anti-inflammation effects on rats, mice and human [7,36,102,102], and the contributive molecules may be flavonols, flavanols and procyanidins (oligomeric flavonoids) [7,36,102]. Bralley et al .…”

Section: Bioactivity Of Phenolic Compounds From Grapementioning

confidence: 99%

Biological Activities of Polyphenols from Grapes

Xia

Deng

Guo

et al. 2010

IJMS

819

345

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The dietary consumption of grape and its products is associated with a lower incidence of degenerative diseases such as cardiovascular disease and certain types of cancers. Most recent interest has focused on the bioactive phenolic compounds in grape. Anthocyanins, flavanols, flavonols and resveratrol are the most important grape polyphenols because they possess many biological activities, such as antioxidant, cardioprotective, anticancer, anti-inflammation, antiaging and antimicrobial properties. This review summarizes current knowledge on the bioactivities of grape phenolics. The extraction, isolation and identification methods of polyphenols from grape as well as their bioavailability and potential toxicity also are included.

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The in vitro effect of a lyophilized extract of wine obtained from Jacquez grapes on human chondrocytes

Cited by 33 publications

References 9 publications

Procyanidins Mitigate Osteoarthritis Pathogenesis by, at Least in Part, Suppressing Vascular Endothelial Growth Factor Signaling

Procyanidins Mitigate Osteoarthritis Pathogenesis by, at Least in Part, Suppressing Vascular Endothelial Growth Factor Signaling

Recent Advances and Uses of Grape Flavonoids as Nutraceuticals

Biological Activities of Polyphenols from Grapes

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